[Home ] [Archive]   [ فارسی ]  
:: ::
:: Volume 12, Issue 1 (March 2018) ::
Qom Univ Med Sci J 2018, 12(1): 44-53 Back to browse issues page
Genotyping of Clinical Isolates of Salmonella enterica serovar Typhimurium from Medical Centers of Kerman Province Using ERIC-PCR Method
Abolfazl Moghadam1 , Shahram Nazarian 2
1- University of Tehran
2- Imam Hossain University , nazarian56@gmail.com
Abstract:   (4163 Views)
Background and Objectives: Salmonella enterica serovar Typhimurium is one of the most common Salmonella serotypes in gastroenteritis cases. ERIC-PCR method is used for genotyping studies and examinations in order to apply appropriate preventive and controlling conditions. This research was carried out with the objective of investigating the genotyping of Salmonella typhimurium strains in the treatment centers of Kerman province.
Methods: In this descriptive study, different strains of Salmonella Typhimurium, were isolated from different treatment centers. All strains were examined using standard methods of microbiology, biochemistry, and molecular biology. Genetic relationship between the strains was evaluated by ERIC-PCR method.
Results: In this study, Among 891 stool and blood samples of patients with diarrhea, 48 strains of Salmonella typhimurium, were isolated. The isolates were classified in terms of genotyping into 15 different groups using ERIC-PCR method, which the highest number of the strains was in the 14th group (25%, 12 strains).
Conclusion: The results of the present study showed high discrimination power of ERIC-PCR method for molecular typing and genetic diversity of Salmonella typhimurium strains isolated from human samples. Therefore, this method can be used for epidemiological studies in order to investigate the source of contamination, genetic diversity and its relationship with geographical distribution and drug resistance of the isolated strains.
Keywords: Salmonella Typhimurium, genetic variation, molecular typing
Full-Text [PDF 824 kb]   (764 Downloads)    
Type of Study: Original Article | Subject: میکروب شناسی
Received: 2016/10/30 | Accepted: 2017/01/28 | Published: 2018/03/11
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Moghadam A, Nazarian S. Genotyping of Clinical Isolates of Salmonella enterica serovar Typhimurium from Medical Centers of Kerman Province Using ERIC-PCR Method. Qom Univ Med Sci J. 2018; 12 (1) :44-53
URL: http://journal.muq.ac.ir/article-1-1263-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 12, Issue 1 (March 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
Persian site map - English site map - Created in 0.07 seconds with 30 queries by YEKTAWEB 4366