[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 11, Number 5 (July 2017) ::
Qom Univ Med Sci J 2017, 11(5): 98-106 Back to browse issues page
Investigation of Antibiotic Susceptibility Pattern of Staphylococcus aureus Strains Isolated from Patients Referring to Some Treatment Centers of Qom City, Iran
Sara Mosaei1, Roohollah Fateh2, Ebrahim Eshaghi1, Mohammad Khalifeh-Gholi *3
1- Department of Biology, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran.
2- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.
3- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. , mkhgh2000@gmail.com
Abstract:   (194 Views)

Background and Objectives: Staphylococcus aureus is one of the important causes of nosocomial infections. Resistance to various antibiotics, such as beta-lactams, aminoglycosides, and macrolides is one of the major problems in treatment and prevention of infections caused by this bacterium. Therefore, accurate determination of antibiotic susceptibility pattern of organisms isolated from patients can be beneficial in treatment and prevention of dangerous infections. The objective of this study was to isolate S. aureus bacterium and to determine the antibiotic susceptibility pattern of the isolated strains in patients referred to some treatment centers of Qom city.

Methods: In this descriptive cross-sectional study, 340 clinical samples, were collected from September 2016 to July 2017. After isolation and primary identification of S. aureus isolates (using standard bacteriology methods), the isolated strains were confirmed by PCR technique and amplification of femA gene as a molecular diagnostic marker of S. aureus. Finally, antibiotic susceptibility pattern of the strains, was determined by disk diffusion method according to CLSI guideline.

Results: Out of 340 clinical samples, 86 S. aureus strains were isolated and identified based on phenotypic characteristics. The femA gene was observed only in 45 strains (52.32%) based on molecular analysis. The results of the antibiotic susceptibility test showed that the highest resistance was to penicillin (86.04%) and the lowest resistance was to chloramphenicol (0%).

Conclusion: The results of this study indicated that femA gene cannot by itself identify all the S. aureus strains. Also, with regard to the results of antibiogram test, it seems that antibiotic susceptibility test is necessary for S. aureus strains isolated from patients.

Keywords: Staphylococcus aureus, Microbial sensitivity test, femA gene, Methicillin-resistant Staphylococcus aureus.
Full-Text [PDF 629 kb]   (156 Downloads)    
Type of Study: Original Article | Subject: میکروب شناسی
Received: 2017/01/15 | Accepted: 2017/02/13 | Published: 2017/07/19
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mosaei S, Fateh R, Eshaghi E, Khalifeh-Gholi M. Investigation of Antibiotic Susceptibility Pattern of Staphylococcus aureus Strains Isolated from Patients Referring to Some Treatment Centers of Qom City, Iran. Qom Univ Med Sci J . 2017; 11 (5) :98-106
URL: http://journal.muq.ac.ir/article-1-1423-en.html
Volume 11, Number 5 (July 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
Persian site map - English site map - Created in 0.185 seconds with 800 queries by yektaweb 3455