[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 12, Issue 4 (June 2018) ::
Qom Univ Med Sci J 2018, 12(4): 81-88 Back to browse issues page
Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran)
Ali Asgari 1 , Razieh Nazari * 2, Seyed Mohammad hosein Razavian 1
1- Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
2- Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran. , nazari1102002@yahoo.com
Abstract:   (396 Views)
Background and Objectives: Mycoplasmas hominis is one of the important causes of bacterial infections of the reproductive system, which through changes in the volume, pH, motility, morphology, and sperm count in men, can lead to reduction in the level of fertility or infertility and sterility. In this research, the frequency of Mycoplasma hominis, was assessed by PCR method, and biological parameters, were investigated in the semen samples of infertile men referred to Qom jihad daneshgahi infertility treatment center.
 
Methods: In this descriptive-analytic study, a total of 187 semen samples were collected from infertile men referred to Qom jihad daneshgahi infertility treatment center from April to September 2016. Semen analysis was performed according to the guideline of World Health Organization. After the extraction of genomic DNA, detection of Mycoplasma hominis, was performed by PCR method using 16S rRNA specific primers. Data were analyzed using dependent and independent t-test.
 
Results: Among 187 semen samples studied by PCR, 71 samples (38%), were positive for Mycoplasma hominis. In the assessment of parameters, there was a significant association between bacterial infection and C class mean difference (in situ sperm motility( (p=0.008).
 
Conclusion: The results of the present study showed that high percentage of infertile men are infected with Mycoplasma hominis, which may lead to pelvic inflammatory diseases, urethritis, and infertility. PCR method is a rapid and efficient technique for detection of mycoplasma, which can be useful in the control of sexual transmitted diseases.
 
 
Keywords: Mycoplasma hominis, Infertility, Polymerase chain reaction.
Full-Text [PDF 413 kb]   (154 Downloads)    
Type of Study: Original Article | Subject: میکروب شناسی
Received: 2017/04/16 | Accepted: 2017/07/16
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Asgari A, Nazari R, Razavian S M H. Investigation of Frequency of Mycoplasma hominis and Biological Parameters in Semen Sample of Men Referred to Qom Jihad Daneshgahi Infertility Treatment Center in 2016 (Iran). Qom Univ Med Sci J . 2018; 12 (4) :81-88
URL: http://journal.muq.ac.ir/article-1-1584-en.html


Volume 12, Issue 4 (June 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
Persian site map - English site map - Created in 0.2 seconds with 31 queries by YEKTAWEB 3781