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:: Volume 13, Issue 7 (September 2019) ::
Qom Univ Med Sci J 2019, 13(7): 10-21 Back to browse issues page
Effect of the Xenograft Transplantation of Human Dental Pulp Stem Cells on Anxiety and Memory in Trimethyltin Induced-Alzheimer Disease Model
Samira Malekzadeh1 , Mohammad Amin Edalatmanesh 2, Davood Mehrabani3 , Mehrdad Shariati4
1- Department of Biology, School of Sciences, Fars Science & Research Branch, Islamic Azad University
2- Department of Biology, School of Sciences, Shiraz Branch, Islamic Azad University , amin.edalatmanesh@gmail.com
3- Stem Cell & Transgenic Technology Research Center, Shiraz University of Medical Sciences
4- Department of Biology, Kazerun Branch, Islamic Azad University
Abstract:   (150 Views)
Background and Objectives: Alzheimer's disease (AD) is a neurodegenerative disease and the most common cause of dementia. The characteristics of this disease are amnesia, cognitive and verbal disorders, memory deficit, paranoia, depression, and anxiety. In this study, the alterations of cognitive-behavioral and nuclear factor kappa (NF-κ) factor, were investigated following the xenogenic transplantation of human dental pulp of stem cells on the AD animal model.
 
Methods: In this experimental study, 18 male Wistar rats (weighing 200±20g), were used. The animals were randomly divided into three groups of 6 each: control (without any treatment), sham (TMT+PBS), and experimental (TMT+DPSCs). The experimental and sham groups received trimethyltin (TMT) intraperitoneally (dose, 8mg/kg bw). Then, the anxiety level, working and spatial memory, were assessed with Elevated Plus and Y Maze tests. Also, the level of NF-κ was assessed using ELISA method.
 
Results: Intraperitoneal administration of TMT caused a significant increase (p<0.05) in the time spent in closed arm of the elevated plus maze and a significant decrease in the alteration behavior and spatial memory in the sham group compared to the control group. On the other hand, following stem cell transplantation, a significant decrease was observed in the anxiety level and working and spatial memory in the experimental group compared to the sham group. Also, the NF-κ level significantly increased in the sham group in comparison with the control and experimental groups (p<0.05).
 
Conclusion: Xenogenic transplantation of the human dental pulp stem cells has an improvement effect on the symptoms of AD. Thus, transplantation of this stem cells line is promising for the treatment of cognitive and psychological diseases.
Keywords: Stem Cells, Alzheimer disease, Anxiety
Full-Text [PDF 1193 kb]   (38 Downloads)    
Type of Study: Original Article | Subject: فیزیولوژی
Received: 2018/01/28 | Accepted: 2018/07/29
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Malekzadeh S, Edalatmanesh M A, Mehrabani D, Shariati M. Effect of the Xenograft Transplantation of Human Dental Pulp Stem Cells on Anxiety and Memory in Trimethyltin Induced-Alzheimer Disease Model. Qom Univ Med Sci J. 2019; 13 (7) :10-21
URL: http://journal.muq.ac.ir/article-1-2010-en.html


Volume 13, Issue 7 (September 2019) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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