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:: Volume 11, Issue 1 (March 2017) ::
Qom Univ Med Sci J 2017, 11(1): 39-48 Back to browse issues page
Molecular detection of genes encoding AcrAB , Qep A efflux pumps in Klebsiella pneumoniae strains isolated from hospitalized patients in selected hospitals in Tehran
Mohsen Heidary *1, Hossein Goudarzi 2, Ali Hashemi 3, Gita Eslami 2, Saeed Khoshnood 4, Shokouh Amraei 5
1- MSc Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , MohsenHeidary40@gmail.com
2- Professor Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- assistant professor Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- MSc Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- MSc Ahvaz Jundishapur University of Medical Sciences
Abstract:   (422 Views)

Abstract

Background and Objectives: Increasing emergence of fluoroquinolone resistance among clinical isolates of Klebsiella pneumoniae  (K. pneumoniae), has limited the treatment options for treatment of infections caused by these bacteria. The aim of this study was to investigate the dissemination of genes encoding AcrAB and QepA efflux pumps among K. pneumoniae strains.

Methods: This study was carried out on 117 K. pneumoniae strains isolated from patients hospitalized in selected hospitals in Tehran city, 2015-2016, Iran. Antimicrobial susceptibility tests were performed using disk diffusion method (based on CLSI guidelines) and identification of acr A, acr B and qep A genes using PCR assay.

Results: In this study, colistin and tigecycline had the best effect against clinical isolates of K. pneumoniae. According to PCR results, 110 (94%) isolates had acrA gene and 102 (87%) isolates had acrB gene, respectively. The qepA gene was not found in any of the K. pneumoniae strains.

Conclusion: According to the results of the present study, dissemination of the genes encoding AcrAB efflux pumps among K. pneumoniae strains, which cause resistance to fluoroquinolones, is a matter of concern. Therefore, infection control and prevention of the spread of drug-resistant bacteria requires careful management in drug prescription and identification of resistant isolates.

Keywords: Klebsiella pneumonia, AcrAB efflux pump, Fluoroquinolones, Microbial sensitivity tests
Full-Text [PDF 477 kb]   (270 Downloads)    
Type of Study: Original Article | Subject: میکروب شناسی
Received: 2016/03/12 | Accepted: 2016/04/7 | Published: 2017/03/14
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Heidary M, Goudarzi H, Hashemi A, Eslami G, Khoshnood S, Amraei S. Molecular detection of genes encoding AcrAB , Qep A efflux pumps in Klebsiella pneumoniae strains isolated from hospitalized patients in selected hospitals in Tehran. Qom Univ Med Sci J . 2017; 11 (1) :39-48
URL: http://journal.muq.ac.ir/article-1-432-en.html
Volume 11, Issue 1 (March 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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