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:: Volume 11, Number 1 (March 2017) ::
Qom Univ Med Sci J 2017, 11(1): 10-17 Back to browse issues page
Effect of Aflatoxin B1 on Histomorphometric Parameters of Testis in Male Mature Albino Mouse
mr Touraj Zamir Nasta1, mr Mazdak Razi *2, Shapour Hassanzadeh3, Hassan Malekinejad3
1- phd Faculty of Veterinary Medicine, Urmia University
2- MSc Faculty of Veterinary Medicine, Urmia University , mazdak.razi@gmail.com toraj.nasta@gmail.com
3- Faculty of Veterinary Medicine, Urmia University
Abstract:   (328 Views)

Background and Objectives: Aflatoxin B1 (AFB1) is a mycotoxin, which is produced by Aspergillus flavus and Aspergillus parasiticus. Among four types of AFB (AFB1, AFB2, AFB3, and AFN4), AFB1 is the most abundant and toxic in the world. Aflatoxin decreases sperm production and quality. In the present study, the effect of ABF1 was investigated on spermatogenesis, spermiogenesis, and cell apoptosis in the testicular tissue.

Methods: In this experimental study, 24 albino mice were divided into 4 groups; control group intraperitoneally received 0.2 ml corn oil and experimental groups received AFB1 (20 µg) for 7, 15, and 35 days, respectively. After 7, 15 and 35 days, the testicular tissue samples were taken, and after preparing tissue sections, hematoxylin and eosin (H&E) staining was performed. Histomorphometric parameters and DNA damage was evaluated using DNA Ladder method. Data analysis was carried out using one-way ANOVA at the significance level of p<0.05.

Results: In this study, AFB1 led to tissue damage, especially in germ cells line. The percentage of seminiferous tubules with differentiation indices, tubular repopulation, and negative spermiogenesis, increased in the mice received AFB1; seminiferous tubules diameter and germinal epithelium thickness, significantly decreased (p<0.05). Finally, AFB1 increased the DNA damages.

Conclusion: The findings of this study revealed that AFB1 increases testicular cell apoptosis via DNA damages, also it can increase differentiation indices, tubular differentiation, and spermiogenesis in testicular tissue, which consequently lead to decrease in sperm production and quality.

Keywords: Aflatoxin B1, Spermatogenesis, Spermiogenesis, DNA damage, Mice
Full-Text [PDF 474 kb]   (338 Downloads)    
Type of Study: Original Article | Subject: علوم آزمایشگاهی
Received: 2016/03/12 | Accepted: 2016/03/15 | Published: 2017/03/14
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Zamir Nasta T, Razi M, Hassanzadeh S, Malekinejad H. Effect of Aflatoxin B1 on Histomorphometric Parameters of Testis in Male Mature Albino Mouse. Qom Univ Med Sci J . 2017; 11 (1) :10-17
URL: http://journal.muq.ac.ir/article-1-435-en.html
Volume 11, Number 1 (March 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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