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:: Volume 11, Issue 1 (March 2017) ::
Qom Univ Med Sci J 2017, 11(1): 57-67 Back to browse issues page
An Investigation of Toxic Shock Syndrome Toxin-1 Gene in Methicillin-Resistant Clinical Strains of Staphylococcus aureus using Multiplex PCR Method
Mohammad Bokaiean 1, Hamed Tahmasebi 2, Shahram Shahraki zahedani *3, Javad Adabi 4
1- Associate professor Zahedan University of Medical Sciences
2- 3 MSc of microbiology Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
3- 3 MSc of microbiology Zahedan University of Medical Sciences , h.tahmasebi87@yahoo.com
4- Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Abstract:   (383 Views)

Background and Objectives: Toxins produced by the bacteria are one of the most common cases, which can, together with other bacterial pathogens, cause or aggravate the disease. One of the diseases caused by bacterial toxins, is toxic shock syndrome. The tst gene encodes this toxin that can be easily transferred between different strains of Staphylococcus aureus. In this study, toxic shock syndrome toxin-1 gene was investigated in methicillin-resistant clinical strains of Staphylococcus aureus using multiplex PCR method.

Methods: This study is a cross-sectional study, during a 9 month period, 470 samples were collected from patients hospitalized in different wards of treatment centers of Zahedan University of Medical Sciences in 2015. Phenotypic method was used for isolation and initial screening. Oxacillin and Cefoxitin discs were used. After isolation of resistant strains, femA and mecA genes and tst gene were investigated using phenotypic method and multiplex PCR method, respectively.

Results: Of 170 clinical isolates of Staphylococcus aureus, 93 isolates were phenotypically methicillin-resistant, among which 89 isolates had mecA gene and 14 isolates had tst gene.

Conclusion: The results indicated that the prevalence of methicillin-resistant strains and the strains carrying causative gene for TSST1, is high in Zahedan. Also, circulation of these isolates can lead to much more severe effects in individuals with weak immune system.

Keywords: MRSA, Enterotoxin, Toxic shock syndrome toxin-1, Staphylococcus aureus
Full-Text [PDF 463 kb]   (231 Downloads)    
Type of Study: Original Article | Subject: میکروب شناسی
Received: 2016/03/15 | Accepted: 2016/04/9 | Published: 2017/03/15
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bokaiean M, Tahmasebi H, shahraki zahedani S, adabi J. An Investigation of Toxic Shock Syndrome Toxin-1 Gene in Methicillin-Resistant Clinical Strains of Staphylococcus aureus using Multiplex PCR Method. Qom Univ Med Sci J . 2017; 11 (1) :57-67
URL: http://journal.muq.ac.ir/article-1-451-en.html
Volume 11, Issue 1 (March 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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