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:: Volume 11, Number 1 (March 2017) ::
Qom Univ Med Sci J 2017, 11(1): 18-28 Back to browse issues page
The Effect of Soy Isoflavone on the Proliferation and Differentiation of Adipose-Derived Mesenchymal Stem Cells into Chondrocytes and Expression of Collagen II and Aggrecan Genes
Fatemeh Bamdadpasand Shekarsarayi1, Mohsen Eslami Farsani *2, Nasrin Heydariya1
1- Faculty of Medicine, Qom Branch, Islamic Azad University
2- Faculty of Medicine, Qom Branch, Islamic Azad University , taramohsen@yahoo.com
Abstract:   (293 Views)

Background and Objectives: Due to the lack of blood vessels in cartilage tissue, its damage is not repairable. This study was conducted to investigate the effect of soy isoflavone on proliferation and differentiation of adipose-derived mesenchymal stem cells into chondrocytes and expression of collagen II and aggrecan genes.

Methods: In this experimental study, human subcutaneous fat was obtained during liposuction and incubated with collagenase enzyme (type 1) for the breakdown of collagen, and collagenase was deactivated by DMEM medium, and was cultured in the cell sediment after centrifugation, the cells were isolated after the third passage, were placed in chondrogenic medium for differentiate into the cartilage, and were divided into three groups, including control, treatment with TGF-β1, and treatment with soy isoflavones tablets. The tablets were dissolved in distilled water, sterilized by passing through a 0.2 um filter and were added to the culture medium. After 48 hours, cell viability was determined by MTT assay, and after 14 days, collagen II and aggrecan gene expressions were assessed by real-time PCR technique. Data were statistically analyzed by one-way ANOVA and Tukey's post-hoc test using SPSS 20 and p<0.05.

Results: The results of MTT assay showed a significant increase in viability in the TGF-β1 group compared to the control and soy isoflavone groups (p<0.05). The RT-PCR indicated a significant increase in the expression of collagen II and aggrecan genes in isoflavones and TGF-β1 groups compared to the control group, and also, the mean CT associated with collagen II gene had a significant increase in isoflavone and TGF-β1groups compared to the control group (p<0.05).

Conclusion: Soy in culture medium increases the expression of collagen II and aggrecan genes and cell proliferation, but this increase is not high compared to the TGF-β1 group.

Keywords: Soy isoflavone aglycone, Mesenchymal stromal cells, Chondrocytes, Collagen type II, Aggrecans
Full-Text [PDF 690 kb]   (209 Downloads)    
Type of Study: Original Article | Subject: سلولی و مولکولی
Received: 2016/04/10 | Accepted: 2016/04/24 | Published: 2017/03/14
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Bamdadpasand Shekarsarayi F, Eslami Farsani M, Heydariya N. The Effect of Soy Isoflavone on the Proliferation and Differentiation of Adipose-Derived Mesenchymal Stem Cells into Chondrocytes and Expression of Collagen II and Aggrecan Genes. Qom Univ Med Sci J . 2017; 11 (1) :18-28
URL: http://journal.muq.ac.ir/article-1-678-en.html
Volume 11, Number 1 (March 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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