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:: Volume 11, Issue 12 (February 2018) ::
Qom Univ Med Sci J 2018, 11(12): 1-9 Back to browse issues page
Effect of AcrAB Efflux Pump on Ciprofloxacin Resistance Rate in Escherichia coli Isolates
Alisha Akya 1, Roya Chegenelorestani * 2, Azam Elahi 3
1- Associate Professor 1Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2- 2MSc student of medical microbiology 1Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. , lorestani25@gmail.com
3- 1Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Abstract:   (619 Views)
Background and Objectives: One of the important mechanisms of resistance to fluoroquinolones in Escherichia coli, is AcrAB efflux pumps activity. In this study, the frequency of acrA and acrB genes and the effect of efflux pumps on the ciprofloxacin resistance in isolates of extended-spectrum beta-lactamase (ESBL) producing E. coli, were investigated.
 
Methods: This experimental study was performed on 66 ESBL producing E.­coli strains isolated from urine sample of outpatients referred to two medical centers in Kermanshah city. Antibiotic susceptibility of the isolates to 10 common antibiotics, was assessed using standard disk diffusion method (according to the CLSI guideline). The acrA and acrB genes were detected by PCR in ciprofloxacin resistant isolates. The inhibitory effect of bacterial efflux pump (carbonyl cyanide m-chlorophenyl hydrazone (CCCP), with final concentration of 25μg/ml on MIC of the ciprofloxacin resistant isolates, was assessed using agar dilution method. The data were analyzed using Chi-square test.
 
Results: Out of 66 isolates, 45 isolates (68.1%), were resistant to ciprofloxacin. Both acrA and acrB genes, were found in the ciprofloxacin resistant E. coli isolates. The effect of CCCP on the resistance of isolates to ciprofloxacin led to the decrease in MIC only in 7 isolates (15.5%).
 
Conclusion: The results of this study showed that despite the high resistance to ciprofloxacin and the presence of both acrA and acrB genes in most of the E.coli isolats, the efflux pump activity exists in a small percentage of isolates. Therefore, efflux pumps play a small role in ciprofloxacin resistance in E. coli.
 
 
 
Keywords: Escherichia coli, Microbial drug resistance, Ciprofloxacin.
Full-Text [PDF 684 kb]   (216 Downloads)    
Type of Study: Original Article | Subject: میکروب شناسی
Received: 2016/10/5 | Accepted: 2016/12/4 | Published: 2018/02/19
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Akya A, chegenelorestani R, elahi A. Effect of AcrAB Efflux Pump on Ciprofloxacin Resistance Rate in Escherichia coli Isolates. Qom Univ Med Sci J . 2018; 11 (12) :1-9
URL: http://journal.muq.ac.ir/article-1-1214-en.html


Volume 11, Issue 12 (February 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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