[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 11, Issue 10 (December 2017) ::
Qom Univ Med Sci J 2017, 11(10): 88-97 Back to browse issues page
Investigation of MexAB-OprM Efflux Pump Expression Level in Pseudomonas aeruginosa Strains Isolated from Patients Hospitalized in Shahid Motahari Hospital in Tehran City using Real-Time PCR method (Iran)
Mehrzad Sadredinamin 1, Hossein Goudarzi 2, Ali Hashemi *, Samira Tarashi 2, Soroor Erfanimanesh 3, Aref Shariati 2, Zohreh Ghalavand 2, Shervin Shokouhi 1, Saeed Shams 4, Neda Yousefi Nojookambari 2
1- 1Infectious Diseases & Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- 2Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- 3Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4- Cellular & Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
Abstract:   (159 Views)
Background and Objectives: Drug resistance in Pseudomonas aeruginosa strains has become a worldwide problem, and efflux pumps are one of the major resistance mechanisms in this bacterium. The aim of this study was to identify the mutation in mexA and mexB pumps and to investigate the expression level of mexA pump in the isolates from burn patients.
 
Methods: This study was conducted as a descriptive study on 100 isolates of P. aeruginosa isolated from patients hospitalized in Shahid Motahari Hospital during 2014-2015. Antibiogram tests were performed using disc diffusion (according to CLSI guidelines). The inhibitory effect of carbonyl cyanide 3-chlorophenylhydrazone (CCCP), was assessed by broth microdilution method. Mutation in mexA and mexB genes, was detected by PCR and sequencing, and the expression level of mexA gene, was evaluated by real-time PCR and 2−ΔΔCT formula.
 
Results: Ninety-five out of 100 isolates of P. aeruginosa, were resistant to imipenem. Sixteen isolates had response to the inhibitory effect, and a 4-fold reduction was observed in MIC results at the presence of this inhibitor. In one isolate, glycine was replaced by aspartic acid at position 257 of mexB protein sequence, but no change was seen in MexA. All isolates had mexA and mexB genes. Twenty percent of the isolates showed overexpression in mexA gene.
 
Conclusion: According to the results of this study, the antibiotic resistance as a result of overexpression of efflux pump, is of great concern. Hence, infection control by accurate management of drug prescription and identification of resistant isolates, is essential to prevent the spread of resistance.
 
 
Keywords: Pseudomonas aeruginosa, Microbial, Real-Time Polymerase Chain Reaction.
Full-Text [PDF 878 kb]   (76 Downloads)    
Type of Study: Original Article | Subject: میکروب شناسی
Received: 2017/03/20 | Accepted: 2017/06/13 | Published: 2017/12/18
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadredinamin M, Goudarzi H, Hashemi A, Tarashi S, Erfanimanesh S, Shariati A, et al . Investigation of MexAB-OprM Efflux Pump Expression Level in Pseudomonas aeruginosa Strains Isolated from Patients Hospitalized in Shahid Motahari Hospital in Tehran City using Real-Time PCR method (Iran). Qom Univ Med Sci J . 2017; 11 (10) :88-97
URL: http://journal.muq.ac.ir/article-1-1557-en.html
Volume 11, Issue 10 (December 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
Persian site map - English site map - Created in 0.185 seconds with 827 queries by yektaweb 3567