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:: Volume 3, Issue 4 (5-2009) ::
Qom Univ Med Sci J 2009, 3(4): 12-19 Back to browse issues page
Neurotrophic Effects of Silibinin on Differentiation of Hair Follicle Stem Cells to Neurons
S Asalgoo 1, M Nobakht * 2, N Rahbar Roshandel 1, K Mousavizadeh 1, N Najafzadeh 1, B Kordestani Shargh 1
1- Iran University of Medical Sciences
2- Iran University of Medical Sciences , manob@iums.ac.ir
Abstract:   (1338 Views)

Background and Objectives: Hair Follicle Bulge region due to its availability and abundance is one of the areas which is easily accessible to Multi-potent stem cells that expresses Nestin marker (neuronal stem cells protein). Stem cells bulge region in hair follicle stem cells has high potency to be differentiated to neuronal cells. Silibinin as an active component of Silybum marianum has anti-oxidant, anti-inflammatory, anti-carcinogenic, hepatoprotective, neurotrophic and neuroprotective effects. The aim of the present study was to evaluate the neurotrophic effects of silibinin on differentiation of hair follicle stem cells to neurons. Methods: Bulge area of whiskers in Rat was isolated and cultivated three weeks in supplemented DMEM/F12 and epidermal growth factor (EGF). Then the cells were exposed over the concentrations of 0.05μg/ml, 0.1μg/ml, 0.4μg/ml, 0.5µg/ml, 0.7µg/ml Silibinin and Neurotrophin-3. Two weeks after culture, plated bulge cells were immunostained with Nestin and differentiated stem cells were immunostained with β III tubulin by immunocytochemistry techniques. The results were evaluated by T-test student analysis. A Pvalue less than 0.05 was considered significant. Result: The nestin marker was clearly demonstrated in bulge regions during the first week, but after two weeks, parallel to stem cells differentiating neuronal cells, β III tubulin marker was expressed in neuronal cells. The toxic effects of 1μg/ml Silibinin on stem cells were also demonstrated, and it stopped the cell growth at the end of the first week. The maximum differentiation on stem cells in 0.5μg/ml Silibinin was observed to be significant (P<0.05). Silibinin concentration increase led to reduced differentiation. Silibinin with neurotrophin 3 increased the differentiation of stem cells. Conclusion: Silibinin concentrations of 1μg/ml and more have toxic effects on hair follicles stem cell differentiation. Also, silibinin concentrations less than 0.1μg/ml had no effect on proliferation and differentiation hair follicle stem cells. Whereas 0.5μg/ml concentrations had significant effects on the differentiation processes of hair follicle stem cells to neuron.

Keywords: Nestin, β III-Tubulin, Silibinin, Hair Follicle
Full-Text [PDF 1470 kb]   (574 Downloads)    
Type of Study: Original Article |
Received: 2016/02/13 | Accepted: 2016/02/22 | Published: 2016/02/22
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Asalgoo S, Nobakht M, Rahbar Roshandel N, Mousavizadeh K, Najafzadeh N, Kordestani Shargh B. Neurotrophic Effects of Silibinin on Differentiation of Hair Follicle Stem Cells to Neurons. Qom Univ Med Sci J . 2009; 3 (4) :12-19
URL: http://journal.muq.ac.ir/article-1-46-en.html


Volume 3, Issue 4 (5-2009) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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