[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 11, Number 5 (July 2017) ::
Qom Univ Med Sci J 2017, 11(5): 38-52 Back to browse issues page
Optimization, Characterization, and Investigation of Antibacterial Activity of Gold Nanoparticles Biosynthesized by Aqueous Extract of Seidlitzia rosmarinus
Omid Azizian Shermeh *1, Mozhgan Taherizadeh2, Moharam Valizadeh3, Jafar Valizadeh4, Ali Qasemi4, Behrooz Naroei4
1- Medicinal & Ornamental Plants Research Center, University of Sistan & Baluchestan, Zahedan, Iran. , omid_aziziyan@yahoo.com
2- Bacteriology Laboratory, Rural Water & Sewage Company of Sistan & Baluchestan, Zahedan, Iran.
3- Department of Plant Production, Faculty of Agriculture, Higher Educational Complex of Saravan, Saravan, Iran.
4- Department of Biology, Faculty of Basic Sciences, University of Sistan & Baluchestan, Zahedan, Iran
Abstract:   (176 Views)

Background and Objectives: The advantages of biological methods are Low cost, non-toxicity, production of high purity nanoparticles, low time-consuming, and completeness reation time. In the present study, biosynthesis of gold nanoparticles using aqueous extract of Seidlitzia rosmarinus and thier antimicrobial activity, were investigated.

Methods: In this study, after preparing the extract, 2 ml of it was added to 4 ml of HAuCl4.3H2O with concentration of 1mM, and the color of solution immediately changed to purple. The parameters affecting the synthesis of nanoparticles [such as pH of reaction medium, extract volume, concentration of gold (III) salt, temperature, and reaction time], were assessed and optimized using UV-vis spectrophotometry. A transmission electron microscope (TEM) was used to characterize the nanoparticles. The antibacterial activity of nanoparticles against four species of pathogenic bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Salmonella typhimurium), were assessed by agar well diffusion method.

Results: Gold nanoparticles showed the maximum absorbance at 544 nm. It was found that the nanoparticles have spherical shape and their average size was 10-18 nm. Also, the results of antibacterial activities showed that the synthesizied nanoparticles had relatively high antibacterial activity.

Conclusion: In the current study, the aqueous extract of Seidlitzia rosmarinus due to its seccondary components and antioxidant activity, has high potential in reduction of gold metal ions as well as synthesis and stabilization of gold nanoparticles, and these synthesized gold nanoparticles were also relatively high antibactrial activity.

Keywords: Metabolism, Antibacterial activity, Rosmarinus, Nanoparticles.
Full-Text [PDF 882 kb]   (63 Downloads)    
Type of Study: Original Article | Subject: علوم پایه
Received: 2016/05/2 | Accepted: 2016/06/11 | Published: 2017/07/19
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Azizian Shermeh O, Taherizadeh M, Valizadeh M, Valizadeh J, Qasemi A, Naroei B. Optimization, Characterization, and Investigation of Antibacterial Activity of Gold Nanoparticles Biosynthesized by Aqueous Extract of Seidlitzia rosmarinus. Qom Univ Med Sci J . 2017; 11 (5) :38-52
URL: http://journal.muq.ac.ir/article-1-799-en.html
Volume 11, Number 5 (July 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
Persian site map - English site map - Created in 0.196 seconds with 806 queries by yektaweb 3445