:: Volume 12, Issue 2 (April 2018) ::
Qom Univ Med Sci J 2018, 12(2): 11-18 Back to browse issues page
The Effect of Decreased Activity in the Form of Neuropathic Pain on GSK-3β Gene Expression in Sciatic Nerve Fiber of Male Wistar Rats
Masoud Rahmati 1, Mokhtar Ghanbarzadeh 2, Mohammad Hassan Aghaei 3
1- Department of Sport Sciences, Faculty of Literature & Humanities, Lorestan University, Khorramabad, Iran. , mo69ph@yahoo.com
2- 2Physiology & Pharmacology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
3- 3Department of Exercise Physiology, Faculty of Literature & Humanities, Branch Kerman, Islamic Azad University, Kerman, Iran.
Abstract:   (363 Views)
Background and Objectives: Studies have shown that impairment in kinases in the nervous system is related to many neurodegenerative disease. Glycogen synthase kinase-3β (GSK-3β) is a serine/threonine kinase that participates in regulation of various functions of neurons. Accordingly, GSK-3β is one of the drug targets for nervous system therapies. In this study, the effect of decreased activity in the form of neuropathic pain, was investigated on GSK-3β gene expression in sciatic nerve fiber of male Wistar rats.
Methods: In this experimental study, 12 male Wistar rats (mean weight, 250±30g), were randomly divided into two groups: 1) Decreased activity (SNL: n=5); 2) Control (C: n=5). During and at the end of the protocol, neuropathic pain behavior tests, were performed continuously in the groups. After sixth weeks, the level of GSK-3β gene expression in sciatic nerve, was measured by real time technique and calculated using the 2-ΔΔCT method. Data were analyzed using two-way repeated measures analysis of variance and independent t-test.
Results: After 6 weeks, neuropathic pain behavior tests of mechanical allodynia and thermal hyperalgesia showed that pain threshold was significantly lower in the SNL group than the control group (p≤0.05). In addition, GSK-3β gene expression significantly increased in the sciatic nerve ligation group compared to the control group (p≤0.05).
Conclusion: The results of this study showed that mechanical allodynia, thermal hyperalgesia, and decreased physical activity are related to increased GSK-3β gene expression in sciatic nerve fiber.
Keywords: Neuralgia, Hypokinesia, Glycogen synthase kinase-3 beta.
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Type of Study: Original Article | Subject: فیزیولوژی ورزشی
Received: 2016/05/31 | Accepted: 2017/01/9 | Published: 2018/04/15

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Volume 12, Issue 2 (April 2018) Back to browse issues page