Qom University of Medical Sciences Journal

Background and Objectives: The Use of medicinal plants in the treatment of diabetesis of particular clinical importance. Reproductive changes has been reported in Diabetic males. The aim of this study was to determine the effects of Descurainia sophia seed on serum testosterone concentration, histology, and sperm parameters of testis tissue. . Methods: In this experimental study, 24 male rats were randomly divided into 3 groups: Healthy control (C) Diabetic control (D), and under treatment diabetic group that received extract of Descurainia sophia (DK) at a dose of 100mg/kg.bw. Diabetic groups became diabetic with intraperitoneal injection of streptozotocin, (65 mg/kg.bw ) and after completion of the treatment period, Heart blood sampling for determining the testosterone level was done for 42 days. Then, all rats were sacrificed and after dissection, sperms were collected from cauda epididymis , and their parameters, including count and morphology were evaluated. Testis samples were transferred into formalin and stained with hematoxylin-eosin and Sudan Black B. Released spermatozoa were used to analyze sperm parameters such as sperm number and morphology. Data were analyzed using one-way ANOVA and post hoc Tukey test. Significance level was considered p≤0/05.   Results: In this study, D. sophia caused asignificant decrease in interstitial space (p<0.001) and abnormal sperm) p<0.001). Also, significant increases were observed in testosterone levels (p<0.001), seminiferous tubules, thickness of the epithelium (p<0.05), spermiogenesis index (p<0.001), and sperm count )p<0.001) compared to the diabetic and D. sophia.   Conclusion: The results of this research revealed that D. sophia have positive effects in the treatment of destruction effects of diabetes on testis tissue.