[Home ] [Archive]   [ فارسی ]  
:: ::
:: Volume 14, Issue 3 (May 2020) ::
Qom Univ Med Sci J 2020, 14(3): 26-34 Back to browse issues page
In vitro Cytotoxicity Evaluation of Steviosid on Cancerous Liver (Hep G2), Colon (HT29), Breast (MCF7) cells and Normal Kidney Cell (Hek293) in Comparison with Cisplatin
Ahmad Abolhasani1 , Fatemeh Heidari2 , Roghayeh Raminfar3 , Shokoufeh Mousavi3 , Hoda Abolhasani * 4
1- Department of Chemical Engineering, Faculty of Technology and Engineering, Qom Uuniversity, Qom, Iran.
2- Department of Anatomy, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
3- Student Research Committee, Qom university of Medical Sciences, Qom, Iran.
4- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. , hodaabolhasani@gamail.com
Abstract:   (3271 Views)
Background and Objectives: Stevioside, a natural sweetener derived from Stevia plant, has anti-cancer properties. Accordingly, the present study investigated the in vitro effects of stevioside cytotoxicity on cancerous liver (Hep G2), colon (HT29), and breast (MCF7) cells and normal human embryonic kidney cell (Hek293) compared to cisplatin as anticancer drug.
 
Methods: In the present study, cytotoxic activity of stevioside was investigated and compared with cisplatin as a well-known anti-cancer drug. The cytotoxic effects of stevioside and cisplatin on Hep G2, HT29, MCF7, and Hek293 cells, were investigated by MTT assay. IC50 values were calculated by fitting the data in a sigmoidal dose-response curve using non-linear regression analysis for each cell line.
 
Results: Among the studied cancerous cell lines, stevioside showed higher cell growth inhibition on Hep G2 cell line. Stevioside did not have high toxicity on Hek293 normal cell line, but cisplatin had more toxicity in comparison with steviosid on the normal human embryonic kidney cell.
 
Conclusion: The sweetener stevioside, showed less cytotoxic effects compared to cisplatin. Due to its non-toxicity to normal human kidney embryonic cell (Hek293), stevioside can be used as auxiliary treatment regimen in patients with liver, breast, and colon cancers.
Keywords: Stevioside, HT29 Cells, Hep G2 Cells, MCF-7 Cells, Hek293 Cells.
Full-Text [PDF 842 kb]   (743 Downloads)    
Type of Study: Original Article | Subject: سلولی و مولکولی
Received: 2020/02/27 | Accepted: 2020/06/23 | Published: 2020/06/30
References
1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA Cancer J Clin 2018;68(1):7-30. PMID: 29313949 [DOI:10.3322/caac.21442]
2. Cancer search-diet related cancer. Kafoodle. Available at: URL: https://kafoodle.com/blog/cure-cancer-diet-related/161011-being-overweight-and-cancer-types_-_to_upload_3/; 2017. Link
3. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 2015;136(5):E359-86. PMID: 25220842 [DOI:10.1002/ijc.29210]
4. Sarmento-Ribeiro AB, Scorilas A, Goncalves AC, Efferth T, Trougakos IP. The emergence of drug resistance to targeted cancer therapies: clinical evidence. Drug Resist Updat 2019;47:100646. PMID: 31733611 [DOI:10.1016/j.drup.2019.100646]
5. Manoj G, Thampi BS, Leelamma S, Menon PV. Effect of dietary fiber on the activity of intestinal and fecal beta-glucuronidase activity during 1,2-dimethylhydrazine induced colon carcinogenesis. Plant Foods Hum Nutr 2001;56(1):13-21. PMID: 11213165 [DOI:10.1023/A:1008188009174]
6. Espinoza MI, Vincken JP, Sanders M, Castro C, Stieger M, Agosin E. Identification, quantification, and sensory characterization of steviol glycosides from differently processed Stevia rebaudiana commercial extracts. J Agric Food Chem 2014;62(49):11797-804. PMID: 25393842 [DOI:10.1021/jf502878k]
7. Rao GN, Rao PP, Balaswamy K, Satyanarayana A. Antioxidant activity of stevia (Stevia rebaudianaL.) leaf powder and a commercial stevioside powder. J Food Pharm Sci 2014;2(2):32-8. Link
8. Gawel-Beben K, Bujak T, Niziol-Lukaszewska Z, Antosiewicz B, Jakubczyk A, Karas M, et al. Stevia rebaudiana Bert. leaf extracts as a multifunctional source of natural antioxidants. Molecules 2015;20(4):5468-86. PMID: 25826787 [DOI:10.3390/molecules20045468]
9. Ahmed B, Hossain M, Islam R, Kumar Saha A, Mandal A. A review on natural sweetener plant - stevia having medicinal and commercial importance. Agronomski Glasnik 2011;73(1-2):75-92. Link
10. Jyoti J, Kaur M, Mishra V, Mittal A. Sweet future of stevia: a magical sweetener. Asian J Pharm Clin Res 2018;11(2):36-42. Link [DOI:10.22159/ajpcr.2018.v11i2.20295]
11. Deshmukh SR, Kedari VR. Isolation, purification and characterization of sweetners from stevia rebaudiana (Bertoni) for their anticancerous activity against colon cancer. World J Pharm Pharm Sci 2014;3(5):1394-410. Link
12. Geuns JM. Stevioside. Phytochemistry 2003;64(5):913-21. PMID: 14561506 [DOI:10.1016/S0031-9422(03)00426-6]
13. Shivanna N, Naika M, Khanum F, Kaul VK. Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana. J Diabetes Complications 2013;27(2):103-13. PMID: 23140911 [DOI:10.1016/j.jdiacomp.2012.10.001]
14. Ritu M, Nandini J. Nutritional composition of Stevia rebaudiana, a sweet herb, and its hypoglycaemic and hypolipidaemic effect on patients with non-insulin dependent diabetes mellitus. J Sci Food Agric 2016;96(12):4231-4. PMID: 26781312 [DOI:10.1002/jsfa.7627]
15. Mohd-Radzman NH, Ismail WI, Adam Z, Jaapar SS, Adam A. Potential roles of stevia rebaudiana bertoni in abrogating insulin resistance and diabetes: a review. Evid Based Complement Alternat Med 2013;2013:718049. PMID: 24324517 [DOI:10.1155/2013/718049]
16. Abolhasani A, Biria D, Abolhasani H, Zarrabi A, Komeili T. Investigation of the role of glucose decorated chitosan and PLGA nanoparticles as blocking agents to glucose transporters of tumor cells. Int J Nanomed 2019;14:9535-46. PMID: 31824149 [DOI:10.2147/IJN.S228652]
17. Jayaraman S, Manoharan M, Illanchezian S. In-vitro antimicrobial and antitumor activities of stevia rebaudiana (Asteraceae) leaf extracts. Trop J Pharm Res 2008;7(4):1143-9. Link [DOI:10.4314/tjpr.v7i4.14700]
18. Chen J, Xia Y, Sui X, Peng Q, Zhang T, Li J, et al. Steviol, a natural product inhibits proliferation of the gastrointestinal cancer cells intensively. Oncotarget 2018;9(41):26299-308. PMID: 29899860 [DOI:10.18632/oncotarget.25233]
19. Gupta E, Kaushik S, Purwar S, Sharma R, Balapure AK, Sundaram S. Anticancer potential of steviol in MCF-7 human breast cancer cells. Pharmacogn Mag 2017;13(51):345-50. PMID: 28839355 [DOI:10.4103/pm.pm_29_17]
20. Paul S, Sengupta S, Bandyopadhyay TK, Bhattacharyya A. Stevioside induced ROS-mediated apoptosis through mitochondrial pathway in human breast cancer cell line MCF-7. Nutr Cancer 2012;64(7):1087-94. PMID: 23061910 [DOI:10.1080/01635581.2012.712735]
21. Abolhasani A, Heidari F, Noori S, Mousavi S, Abolhasani H. Cytotoxicity evaluation of dimethoxy and trimethoxy indanonic spiroisoxazolines against cancerous liver cells. Curr Chem Biol 2020;14(1):38-48. Link [DOI:10.2174/2212796813666190926112807]
22. Abolhasani H, Zarghi A, Abolhasani A, Hamzeh-Mivehroud M, Bargahi N, Notash B, et al. Design, synthesis and in vitro cytotoxicity evaluation of new 3',4'-bis (3,4,5-trisubstituted)-4'H-spiro[indene-2,5'-isoxazol]-1(3H)-one derivatives as promising anticancer agents. Lett Drug Des Discov 2014;11(10):1149-61. Link [DOI:10.2174/1570180811666140704172442]
23. Ruiz-Ruiz JC, Moguel-Ordoñez YB, Segura-Campos MR. Biological activity of Stevia rebaudiana Bertoni and their relationship to health. Crit Rev Food Sci Nutr 2017;57(12):2680-90. PMID: 26479769 [DOI:10.1080/10408398.2015.1072083]
24. Alijani HQ, Pourseyedi S, Mahani MT, Khatami M. Green synthesis of zinc sulfide (ZnS) nanoparticles using Stevia rebaudiana Bertoni and evaluation of its cytotoxic properties. J Mol Struct 2019;1175:214-8. Link [DOI:10.1016/j.molstruc.2018.07.103]
25. Rajesh P, Rajesh VK, Durai MT. Effect of Stevia rebaudiana Bertoni ethanolic extract on anti-cancer activity of Erlisch's Ascites carcinoma induced mice. Curr Biotica 2010;3(4):549-54. Link
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abolhasani A, Heidari F, Raminfar R, Mousavi S, Abolhasani H. In vitro Cytotoxicity Evaluation of Steviosid on Cancerous Liver (Hep G2), Colon (HT29), Breast (MCF7) cells and Normal Kidney Cell (Hek293) in Comparison with Cisplatin. Qom Univ Med Sci J 2020; 14 (3) :26-34
URL: http://journal.muq.ac.ir/article-1-2753-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 14, Issue 3 (May 2020) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
Persian site map - English site map - Created in 0.06 seconds with 30 queries by YEKTAWEB 4533