[Home ] [Archive]   [ فارسی ]  
:: ::
:: Volume 14, Issue 11 (January 2021) ::
Qom Univ Med Sci J 2021, 14(11): 11-19 Back to browse issues page
Effect of Oleuropein on the Gene Expression of Brain-derived Neurotrophic Factor, Stress Oxidative, and Spatial Memory Impairment Caused by Scopolamine in Male Rats
Gelavij Mahnoodi1 , Azam Moshfegh 2
1- Department of Biology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
2- Department of Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
Abstract:   (1151 Views)
Background and Objectives: Oleuropein is an important phenolic compound found in olive leaves that has important pharmacological functions in the central nervous system and neuroprotective effects in vitro and in vivo conditions. This study was conducted to investigate the improving effects of oleuropein on the formation of scopolamine-induced spatial memory.
Methods: The statistical population of this study consisted of 35 male rats, which were randomly divided into five groups (n=7 each). The control group received normal saline, the scopolamine group was subjected to intraperitoneal scopolamine at a dose of 1 mg/kg for 3 weeks, and the treatment groups were administered oleuropein in three doses of 5, 10, and 20 mg/kg. At the end of the behavioral tests, blood samples and brain tissues (hippocampus) of the animals were removed for biochemical and molecular tests.
Results: Based on the results, scopolamine significantly decreased spatial memory, whereas the injections of different doses of oleuropein improved spatial memory and learning. On the other hand, scopolamine decreased the antioxidant capacity of the serum and hippocampus, while oleuropein significantly increased these values. Our results also revealed that treatment of rats with oleuropein significantly increased the expression of brain-derived neurotrophic factor gene in the hippocampus.
Conclusion: According to the results of this study, oleuropein can be a neuropharmacological agent against scopolamine-induced amnesia.
Keywords: Brain-derived neurotrophic factor, Hippocampus, Oleuropein, Scopolamine, Rats, Spatial memory.
Full-Text [PDF 1143 kb]   (507 Downloads)    
Type of Study: Original Article | Subject: فیزیولوژی
Received: 2020/10/16 | Accepted: 2021/02/14 | Published: 2021/01/29
1. 1. Arendt T, Stieler J, Holzer M. Brain hypometabolism triggers PHF-like phosphorylation of tau, a major hallmark of Alzheimer's disease pathology. J Neural Transm 2015;122(4):531-9. DOI: 10.1007/s00702-014-1342-8 [DOI:10.1007/s00702-014-1342-8]
2. Chen W, Cheng X, Chen J, Yi X, Nie D, Sun X, et al. Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats. PLoS One 2014;9(2):e88076. DOI: 10.1371/journal.pone.0088076 [DOI:10.1371/journal.pone.0088076]
3. Bruel-Jungerman E, Lucassen PJ, Francis F. Cholinergic influences on cortical development and adult neurogenesis. Behav Brain Res 2011;221(2):379-88. DOI: 10.1016/j.bbr.2011.01.021 [DOI:10.1016/j.bbr.2011.01.021]
4. Park HR, Lee H, Park H, Cho WK, Ma JY. Fermented Sipjeondaebo-tang alleviates memory deficits and loss of hippocampal neurogenesis in scopolamine-induced amnesia in mice. Sci Rep 2016;6:22405. DOI: 10.1038/srep22405 [DOI:10.1038/srep22405]
5. Queen BL, Tollefsbol TO. Polyphenols and aging. Curr Aging Sci 2010;3(1)34-42. DOI: 10.2174/1874609811003010034 [DOI:10.2174/1874609811003010034]
6. Rahimi N, Delfan B, Motamed-Gorji N, Dehpour AR. Effects of oleuropein on pentylenetetrazol-induced seizures in mice: involvement of opioidergic and nitrergic systems. J Nat Med 2017;71(2):389-96. DOI: 10.1007/s11418-017-1071-z [DOI:10.1007/s11418-017-1071-z]
7. Pourkhodadad S, Alirezaei M, Moghaddasi M, Ahmadvand H, Karami M, Delfan B, et al. Neuroprotective effects of oleuropein against cognitive dysfunction induced by colchicine in hippocampal CA1 area in rats. J Physiol Sci 2016;66(5):397-405. DOI: 10.1007/s12576-016-0437-4 [DOI:10.1007/s12576-016-0437-4]
8. Dekanski D, Selaković V, Piperski V, Radulović Ž, Korenić A, Radenović L. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 2011;18(13):1137-43. DOI: 10.1016/j.phymed.2011.05.010 [DOI:10.1016/j.phymed.2011.05.010]
9. De La Cruz JP, Del Río S, Arrebola MM, López-Villodres JA, Jebrouni N, González-Correa JA. Effect of virgin olive oil plus acetylsalicylic acid on brain slices damage after hypoxia-reoxygenation in rats with type 1-like diabetes mellitus. Neurosci Lett 2010;471(2):89-93. DOI: 10.1016/j.neulet.2010.01.017 [DOI:10.1016/j.neulet.2010.01.017]
10. Kuem N, Song SJ, Yu R, Yun JW, Park T. Oleuropein attenuates visceral adiposity in high‐fat diet‐induced obese mice through the modulation of WNT10b‐and galanin‐mediated signaling. Mol Nutr Food Res 2014;58(11):2166-76. DOI: 10.1002/mnfr.201400159 [DOI:10.1002/mnfr.201400159]
11. Khalatbary A, GhR Z. Anti-inflammatory effect of oleuropein in experimental rat spinal cord trauma. Iran Red Crescent Med J 2012;14(4):229-34. Link
12. Khalatbary AR, Ghaffari E, Mohammadnegad B. Protective role of oleuropein against acute deltamethrin-induced neurotoxicity in rat brain. Iran Biomed J 2015;19(4):247-53. DOI: 10.7508/ibj.2015.04.009
13. Khalatbary AR. Olive oil phenols and neuroprotection. Nutr Neurosci 2013;16(6):243-9. DOI: 10.1179/1476830513Y.0000000052 [DOI:10.1179/1476830513Y.0000000052]
14. Rabiei Z, Setorki M. Effect of hydroalcoholic Echium amoenum extract on scopolamine-induced learning and memory impairment in rats. Pharm Biol 2018;56(1):672-7. DOI: 10.1080/13880209.2018.1543330 [DOI:10.1080/13880209.2018.1543330]
15. Teymourivand Y, Hooshmandi Z, Setorki M, Amini S. Protective effects of biarum carduchrum ethyl acetate extract on seizure severity, depression, memory, and learning in pentylenetetrazole-induced kindling rats. Iran Red Crescent Med J 2020;22(8):e76. (In Persian) Link
16. Ionita R, Postu PA, Mihasan M, Gorgan DL, Hancianu M, Cioanca O, et al. Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: a behavioral and molecular study. Phytomedicine 2018;47:113-20. DOI: 10.1016/j.phymed.2018.04.049 [DOI:10.1016/j.phymed.2018.04.049]
17. Budzynska B, Boguszewska-Czubara A, Kruk-Slomka M, Skalicka-Wozniak K, Michalak A, Musik I, et al. Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice. Psychopharmacology 2015;232(5):931-42. DOI: 10.1007/s00213-014-3728-6 [DOI:10.1007/s00213-014-3728-6]
18. Markesbery WR. Oxidative stress hypothesis in Alzheimer's disease. Free Radic Biol Med 1997;23(1):134-47. DOI: 10.1016/s0891-5849(96)00629-6 [DOI:10.1016/S0891-5849(96)00629-6]
19. Kwon SH, Lee HK, Kim JA, Hong SI, Kim HC, Jo TH, et al. Neuroprotective effects of chlorogenic acid on scopolamine-induced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice. Eur J Pharmacol 2010;649(1-3):210-7. DOI: 10.1016/j.ejphar.2010.09.001 [DOI:10.1016/j.ejphar.2010.09.001]
20. Khalatbary AR, Ahmadvand H. Neuroprotective effect of oleuropein following spinal cord injury in rats. Neurol Res 2012;34(1):44-51. DOI: 10.1179/1743132811Y.0000000058 [DOI:10.1179/1743132811Y.0000000058]
21. Alirezaei M, Dezfoulian O, Neamati S, Rashidipour M, Tanideh N, Kheradmand A. Oleuropein prevents ethanol-induced gastric ulcers via elevation of antioxidant enzyme activities in rats. J Physiol Biochem 2012;68(4):583-92. DOI: 10.1007/s13105-012-0177-8 [DOI:10.1007/s13105-012-0177-8]
22. Alirezaei M, Dezfoulian O, Sookhtehzari A, Asadian P, Khoshdel Z. Antioxidant effects of oleuropein versus oxidative stress induced by ethanol in the rat intestine. Compar Clin Pathol 2014;23(5):1359-65. DOI: 10.1007/s00580-013-1791-8 [DOI:10.1007/s00580-013-1791-8]
23. Daccache A, Lion C, Sibille N, Gerard M, Slomianny C, Lippens G, et al. Oleuropein and derivatives from olives as Tau aggregation inhibitors. Neurochem Int 2011;58(6):700-7. DOI: 10.1016/j.neuint.2011.02.010 [DOI:10.1016/j.neuint.2011.02.010]
24. Kostomoiri M, Fragkouli A, Sagnou M, Skaltsounis LA, Pelecanou M, Tsilibary EC, et al. Oleuropein, an anti-oxidant polyphenol constituent of olive promotes α-secretase cleavage of the amyloid precursor protein (AβPP). Cell Mol Neurobiol 2013;33(1):147-54. DOI: 10.1007/s10571-012-9880-9 [DOI:10.1007/s10571-012-9880-9]
25. Luccarini I, Dami TE, Grossi C, Rigacci S, Stefani M, Casamenti F. Oleuropein aglycone counteracts Aβ42 toxicity in the rat brain. Neurosci Lett 2014;558:67-72. DOI: 10.1016/j.neulet.2013.10.062 [DOI:10.1016/j.neulet.2013.10.062]
26. Diomede L, Rigacci S, Romeo M, Stefani M, Salmona M. Oleuropein aglycone protects transgenic C. elegans strains expressing Aβ42 by reducing plaque load and motor deficit. PLoS One 2013;8(3):e58893. DOI: 10.1371/journal.pone.0058893 [DOI:10.1371/journal.pone.0058893]
27. Pourkhodadad S, Alirezaei M, Moghaddasi M, Ahmadvand H, Karami M, Delfan B, et al. Neuroprotective effects of oleuropein against cognitive dysfunction induced by colchicine in hippocampal CA1 area in rats. J Physiol Sci 2016;66(5):397-405. DOI: 10.1007/s12576-016-0437-4 [DOI:10.1007/s12576-016-0437-4]
28. Carito V, Venditti A, Bianco A, Ceccanti M, Serrilli AM, Chaldakov G, et al. Effects of olive leaf polyphenols on male mouse brain NGF, BDNF and their receptors TrkA, TrkB and p75. Nat Prod Res 2014;28(22):1970-84. DOI: 10.1080/14786419.2014.918977 [DOI:10.1080/14786419.2014.918977]
Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mahnoodi G, Moshfegh A. Effect of Oleuropein on the Gene Expression of Brain-derived Neurotrophic Factor, Stress Oxidative, and Spatial Memory Impairment Caused by Scopolamine in Male Rats. Qom Univ Med Sci J 2021; 14 (11) :11-19
URL: http://journal.muq.ac.ir/article-1-2954-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 14, Issue 11 (January 2021) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
Persian site map - English site map - Created in 0.07 seconds with 30 queries by YEKTAWEB 4547