Volume 15, Issue 3 (June 2021)
Qom Univ Med Sci J 2021, 15(3): 222-229 |
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1- 1. Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran 2. Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- 3. Isfahan Fertility and Infertility Center, Isfahan, Iran 4. Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
3- 5. Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran ,mforozanfar@yahoo.com
4- 4. Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
2- 3. Isfahan Fertility and Infertility Center, Isfahan, Iran 4. Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
3- 5. Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran ,
4- 4. Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Abstract: (1840 Views)
Background and Objective: Although proper morphology and motility of sperm can be one of the most important factors influencing male fertility, however, these parameters cannot play a decisive role in the health of sperm DNA. As a result, in addition to reducing the quality of sperm parameters in oligospermia individuals, sperm DNA damage or chromatin abnormalities of the sperm nucleus may also play a role in infertility. In this study, the relationship between sperm parameters and sperm DNA damage, and chromatin disorders of the sperm nucleus in oligospermia individuals was investigated.
Methods: This descriptive correlation study was performed on semen samples taken from 40 men with oligozoospermia. Thus, sperm parameters were evaluated. The fragmentation of sperm DNA, histone residue percentage, and sperm protamine deficiency were examined by SCD, aniline blue staining, and chromamycin A3 evaluation, respectively. FSH was assessed through blood samples from patients. Pearson correlation was used for correlation between the parameters. The significance level was considered P<0.05.
Results: In this study, there was a significant correlation between DNA fragmentation and FSH level. Also, chromatin deficiency disorders and DNA fragmentation showed a significant correlation. In addition, there was a significant correlation between excess histone and DNA fragmentation.
Conclusion: In oligospermia, DNA fragmentation is associated with sperm chromatin abnormalities and damage to their sperm DNA, and is a possible cause of infertility.
Methods: This descriptive correlation study was performed on semen samples taken from 40 men with oligozoospermia. Thus, sperm parameters were evaluated. The fragmentation of sperm DNA, histone residue percentage, and sperm protamine deficiency were examined by SCD, aniline blue staining, and chromamycin A3 evaluation, respectively. FSH was assessed through blood samples from patients. Pearson correlation was used for correlation between the parameters. The significance level was considered P<0.05.
Results: In this study, there was a significant correlation between DNA fragmentation and FSH level. Also, chromatin deficiency disorders and DNA fragmentation showed a significant correlation. In addition, there was a significant correlation between excess histone and DNA fragmentation.
Conclusion: In oligospermia, DNA fragmentation is associated with sperm chromatin abnormalities and damage to their sperm DNA, and is a possible cause of infertility.
Keywords: Oligospermia, DNA fragmentation, Sperm nucleus histone chromatin density, Sperm protamine deficiency, Follicle stimulating hormone
Type of Study: Original Article |
Subject:
بافت شناسی و جنین شناسی
Received: 2021/02/21 | Accepted: 2021/04/14 | Published: 2021/07/1
Received: 2021/02/21 | Accepted: 2021/04/14 | Published: 2021/07/1
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