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Qom Univ Med Sci J 2021, 14(12): 14-21 Back to browse issues page
Evaluation of antitrichomonal effect of methanolic extract of Hyssopus officinalis on Trichomonas vaginalis in vitro
Roghayeh Norouzi1 , Jafar Adnani Sadati Seyed 2, Ramin Yaghoubian3 , Farzaneh Mirzaei4 , Ruhollah Fateh5 , Abolghasem Siadatpanah6
1- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
2- Department of Microbiology, Immunology and Parasitology, School of Medicine, Qom University of Medical Sciences, Qom, Iran , jafaradnani@yahoo.com
3- Department of Microbiology, Immunology and Parasitology, School of Medicine, Qom University of Medical Sciences, Qom, Iran.
4- Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5- Department of Microbiology, Immunology and Parasitology, School of Medicine, Qom University of Medical Sciences, Qom, Iran
6- Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran
Abstract:   (233 Views)
Background and Objectives: Trichomonas vaginalis is a flagellate protozoan that is the most common sexually parasitic transmitted infection in the world. Metronidazole is currently used for the treatment of trichomoniasis, which has side effects. In recent years, the use of plants and natural compounds has been considered by researchers. This study was conducted to investigate the anti-trichomonal activity of methanolic extract of Hyssopus officinalis on Trichomonas vaginalis in vitro.
 
Methods: In this experimental study, the extract was prepared by Soxhlet method, and to evaluate the effect of methanolic extract of Hyssopus officinalis on Trichomonas vaginalis parasites, the concentrations of 50, 100, 200, 400, 600, 800, 1,000, 1,200, and 1,400 μg/ml was prepared. Metronidazole and distilled water were considered as positive and negative controls, respectively. Afterward, 105 live parasites were added to all tubes, and all groups were kept at 37°C. Live parasites were counted at 24, 48, and 72 h intervals by Trypan Blue using a neobar slide and light microscope (Hemocytometer). Subsequently, the IC50 value for the above extract was calculated using SigmaPlot ™ software (version 13). It should be noted that all steps of the experiment were performed in triplicate and the results were considered as average.
 
Results: The IC50 of methanolic extract of Hyssopus officinalis on Trichomonas vaginalis parasite was calculated at 965.8, 730.4, and 542.1 μg/ml after 24, 48, and 72 h, respectively. The highest mortality rate (100%) was observed at a concentration of 1,400 μg/ml after 72 h of exposure.
 
Conclusion: Methanolic extract of Hyssopus officinalis in different concentrations had an inhibitory effect on the growth of the Trichomonas vaginalis parasite. Further and more comprehensive studies are suggested to investigate the constituents of this plant and the lethal effect of the parasite in vivo conditions.
Keywords: Hyssopus officinalis, Hyssopus Plant, Methanolic extract, Metronidazole, Trichomonas vaginalis, In vitro Techniques
Full-Text [PDF 704 kb]   (95 Downloads)    
Type of Study: Original Article | Subject: انگل شناسی
Received: 2021/03/1 | Accepted: 2021/03/13 | Published: 2021/02/28
References
1. 1. Dias-Lopes G, Wiśniewski JR, De Souza NP, Vidal VE, Padrón G, Britto C. In-depth quantitative proteomic analysis of trophozoites and pseudocysts of Trichomonas vaginalis. J Proteome Res 2018;17(11):3704-18. DOI: 10.1021/acs.jproteome.8b00343 [DOI:10.1021/acs.jproteome.8b00343]
2. Schwebke JR, Burgess D. Trichomoniasis. Clin Microbiol Rev 2004;17(4):794-803. DOI: 10.1128/CMR.17.4.794-803.2004 [DOI:10.1128/CMR.17.4.794-803.2004]
3. Garber GE. The laboratory diagnosis of Trichomonas vaginalis. Can J Infect Dis Med Microbiol 2005;16(1):35-8. DOI: 10.1155/2005/373920 [DOI:10.1155/2005/373920]
4. Chen YP, Twu O, Johnson PJ. Trichomonas vaginalis macrophage migration inhibitory factor mediates parasite survival during nutrient stress. mBio 2018;9(3):00910-8. DOI: 10.1128/mBio.00910-18 [DOI:10.1128/mBio.00910-18]
5. Arbabi M, Delavari M, Fakhrieh-Kashan Z, Hooshyar H. Review of Trichomonas vaginalis in Iran, based on epidemiological situation. J Reprod Infertil 2018;19(2):82-8. Link
6. McClelland RS, Sangaré L, Hassan WM, Lavreys L, Mandaliya K, Kiarie J. Infection with Trichomonas vaginalis increases the risk of HIV-1 acquisition. J Infect Dis 2007;195(5):698-702. DOI: 10.1086/511278 [DOI:10.1086/511278]
7. Kissinger P, Muzny CA, Mena LA, Lillis RA, Schwebke JR, Beauchamps L. Single-dose versus 7-day-dose metronidazole for the treatment of trichomoniasis in women: an open-label, randomised controlled trial. Lancet Infect Dis 2018;18(11):1251-9. DOI: 10.1016/S1473-3099(18)30423-7 [DOI:10.1016/S1473-3099(18)30423-7]
8. Fakhrieh-Kashan Z, Arbabi M, Delavari M, Mohebali M, Hooshyar H. Induction of apoptosis by alcoholic extract of combination Verbascum thapsus and Ginger officinale on Iranian isolate of Trichomonas vaginalis. Iran J Parasitol 2018;13(1):72-8. Link
9. Azadbakht M, Ziai H, Abdollahi F, Shabankhani B. Effect of essential oils of Artemisia, Zataria and Myrtus on Trichomonas vaginalis. J Med Plants 2003;2(8):35-40. Link
10. Fakhrieh Kashan Z, Delavari M, Arbabi M, Hooshyar H. Therapeutic effects of Iranian herbal extracts against Trichomonas vaginalis. Iran Biomed J 2017;21(5):285-93. DOI: 10.18869/acadpub.ibj.21.5.285 [DOI:10.18869/acadpub.ibj.21.5.285]
11. Khalili B, Rafieian M, Hejazi SH, Yusefi HA, Yektaian N, Shirani-Bidabadi L. Effect of Achillea millefolium, Artemisia absinthium & Juglans regia leaves extracts on Trichomonas vaginalis, in vitro. Shahrekord Univ Med Sci J 2011;12(4):62-9. Link
12. Fakhrie-Kashan Z, Arbabi M, Delavari M, Taghi-Zadeh M, Hooshyar H, Solaymani F. The effect of aqueous and alcoholic extracts of Pelarqonium roseum on the growth of Trichomonas vaginalis in vitro. Feyz 2014;18(4):369-75. Link
13. Moghtader M. Comparative evaluation of the essential oil composition from the leaves and flowers of Hyssopus officinalis L. J Horticulture Forestry 2014;6(1):1-5. DOI 10.5897/JHF2013.0318 [DOI:10.5897/JHF2013.0318]
14. Najafpour NM, Mirza M. Comparative study on the essential oil composition of the leaves of hyssopus officinalis L. in field and wild growing. Iran J Med Arom Plants 2002;18:43-51. Link
15. Arbabi M, Fakhrieh-Kashan Z, Delavari M, Taghizadeh M, Hooshyar H. Effect of alcoholic extracts of Arctium lappa L. and Satureja hortensis L. against Trichomonas vaginalis in vitro. Feyz 2017;21(4):298-304. Link
16. Dunne RL, Dunn LA, Upcroft P, O'Donoghue PJ, Upcroft JA. Drug resistance in the sexually transmitted protozoan Trichomonas vaginalis. Cell Res 2003;13(4):239-49. DOI: 10.1038/sj.cr.7290169 [DOI:10.1038/sj.cr.7290169]
17. Arefkhah N, Taghipur S, Yousefi M, Rafieian-Kopaei M, Daneshpur S. In-vitro effect of hydro-alcoholic extract of tanacetum parthenium extract on Trichomonas vaginalis. J Isfahan Med Sch 2013;31(236):623-9. Link
18. Yousefi M, Taghipur S, Arefkhah N, Rahimian R, Davoudian A, Rafieian-Kopaei M. In-vitro effect of menthe piperita and salvia officinalis extracts on trichomonas vaginalis. J Isfahan Med Sch 2013;31(240):811-8. Link
19. Salehi1 L, Asghari G, Yousofi H, Yousofi-Darani H. The effects of different extracts of viola odorata on Trichomonas vaginalis in culture medium. J Isfahan Med Sch 2014;31(266):2139-48. Link
20. Gollapudi S, Sharma HA, Aggarwal S, Byers LD, Ensley HE, Gupta S. Isolation of a previously unidentified polysaccharide (MAR-10) from Hyssop officinalis that exhibits strong activity against human immunodeficiency virus type 1. Biochem Biophys Res Commun 1995;210(1):145-51. DOI: 10.1006/bbrc.1995.1639 [DOI:10.1006/bbrc.1995.1639]
21. Es-saady D, Simon A, Ollier M, Maurizis JC, Chulia AJ, Delage C. Inhibitory effect of ursolic acid on B16 proliferation through cell cycle arrest. Cancer Lett 1996;106(2):193-7. DOI: 10.1016/0304-3835(96)04312-1 [DOI:10.1016/0304-3835(96)04312-1]
22. Calzada F, Yépez-Mulia L, Tapia-Contreras A. Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites. J Ethnopharmacol 2007;113(2):248-51. DOI: 10.1016/j.jep.2007.06.001 [DOI:10.1016/j.jep.2007.06.001]
23. Rafieian-Kopaei M, Yousofi Darani H, Delaram M, Safdari F, Banaian S, Sereshti M, Zebardast N, et al. Effects of Eucalyptus camaldulensis exracts on Trichimonas vaginalis growth in vitro. J Med Plants 2012;2(42):116-20. Link
24. Matini M, Bakhtiarnejad S, Dastan D, Maghsood AH, Fallah M. In vitro efficacy of plantago lanceolata L. extracts on Trichomonas vaginalis. J Arak Uni Med Sci 2017;20(6):74-82. Link
25. Akbari M, Dastan D, Fallah M, Matini M. In vitro activity of Ferula gummosa essential oil and its different extracts on Trichomonas vaginalis. J Ilam Univ Med Sci 2019;27(2):1-10. DOI: 10.29252/sjimu.27.2.1 [DOI:10.29252/sjimu.27.2.1]
26. Matini M, Bakhtiyarnejad S, Dastan D, Maghsood A, Fallah M. Investigation of in-vitro efficacy of Pistacia atlantica subsp. kurdica extracts against trichomonas vaginalis. J Med Stud 2018;29(3):198-207. Link
27. Ertabaklar H, Kivcak B, Mert T, Ozensoy S. In vitro activity of Arbutus unedo leaf extracts against Trichomonas vaginalis trophozoites. Turkiye Parazitol Derg 2009;33(4):263-5. Link
28. Taran M, Rezaeian M, Izaddoost M. In vitro antitrichomonas activity of Allium hirtifolium (Persian Shallot) in comparison with Metronidazole. Iran J Public Health 2006;35(1):92-4. Link
29. Niyati M, Joneidi Z, Kamalinejad M, Haghighi A, Valaei N, Abdi AR, et al. Anti-trichomonas effect of Rheum ribes and Foeniculum vulgare extracts on Trichomonas vaginalis in vitro. J Islam Iran Traditional Med 2015;6(3):198-208. Link
30. Zarea A, Asghari G, Ghanadian M, Yousefi H, Yousofi Darani H. Effect of Taxus baccata leaves fractions on Trichomonas vaginalis growth in culture medium. Armaghane Danesh 2014;18(11):888-99. Link
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Norouzi R, Adnani Sadati J, Yaghoubian R, Mirzaei F, Fateh R, Siadatpanah A. Evaluation of antitrichomonal effect of methanolic extract of Hyssopus officinalis on Trichomonas vaginalis in vitro. Qom Univ Med Sci J. 2021; 14 (12) :14-21
URL: http://journal.muq.ac.ir/article-1-3078-en.html


Volume 14, Issue 12 (February 2021) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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