Volume 16, Issue 10 (January 2023)                   Qom Univ Med Sci J 2023, 16(10): 816-829 | Back to browse issues page

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Izadi Z, Mirazi N. Antioxidant and Antimicrobial Properties of Ethanolic Extract of Lemon Verbena. Qom Univ Med Sci J 2023; 16 (10) :816-829
URL: http://journal.muq.ac.ir/article-1-3136-en.html
1- Department of Horticultural Sciences and Engineering, Nahavand Higher Education Complex, Bu-Ali Sina University, Hamedan, Iran. , armaghan_iza_2004@yahoo.com
2- Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
Abstract:   (687 Views)
Background and Objectives: Chemical preservatives are commonly used to prevent food from quick spoiling and oxidizing. Despite their effect in increasing shelf life of foods, these compounds can have side effects. Residues of chemical compounds in foods increase microbial resistance. This study aims to investigate the antioxidant and antimicrobial properties of lemon verbena (Lippia citriodora L.) ethanolic extract against some gram-positive and gram-negative bacteria.
Methods: In this experimental study, The soaking method and ethanol solvent was used for extraction. Antioxidant activities of different concentrations of extract were assessed using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity test and compared with butylated hydroxyanisole (BHA) as a synthetic antioxidant. Antimicrobial effect of extract on Staphylococcus aureus, Listeria innocua, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium was determined by disk diffusion and well diffusion methods and measuring minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Experimental data were analyzed in SPSS software, version 20 using analysis of variance and the mean values were compared using Duncan’s multiple range test.
Results: The amounts of phenols and flavonoids in the ethanolic extract of were 96.47±0.35 mg of gallic acid/g of extract and 31.07±0.42 mg of quercetin/g of extract, respectively. The half-maximal inhibitory concentration of lemon verbena extract was 11.79±0.76 μg/mL, while it was 10.16±0.29 μg/mL for BHA. In both disk diffusion and well diffusion methods, with the increase of the concentration of extract, the inhibition zone diameter increased. The ethanolic extract of lemon verbena at the concentration of 12.5 mg/mL had no inhibitory effect on gram-negative bacteria growth. In both methods, the extract had the greatest effect on S. aureus. The MIC of ethanolic extract ranged from 8 to 128 mg/mL, depending on the type of bacteria (gram-positive or gram-negative).
Conclusion: The ethanolic extract of lemon verbena has higher antimicrobial effects on gram-positive bacteria. Therefore, it can be used in food and pharmaceutical industries as a natural antimicrobial agent. Considering the increasing resistance of bacteria to chemical antibiotics, more studies on lemon verbena and its antibacterial compounds are needed for treating infections.
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Type of Study: Original Article | Subject: میکروب شناسی
Received: 2021/05/9 | Accepted: 2022/11/19 | Published: 2023/01/1

1. Fathi A, Sahari M, Zangiabadi M, Barzegar M. [Application of satureja hortensis L and zataria multiflora boiss essential oils as two natural antioxidants in soybean oil during microwave heating (Persian)]. J Med Plants. 2011; 10(39):12-21. [Link]
2. Shojaee-Aliabadi S, Hosseini H, Mohammadifar MA, Mohammadi A, Ghasemlou M, Ojagh SM, et al. Characterization of antioxidant-antimicrobial Κ-carrageenan films containing Satureja hortensis essential oil. Int J Biol Macromol. 2013; 52:116-24. [DOI:10.1016/j.ijbiomac.2012.08.026] [PMID] [DOI:10.1016/j.ijbiomac.2012.08.026]
3. Taleb H, Maddocks SE, Morris RK, Kanekanian AD. The antibacterial activity of date syrup polyphenols against S. aureus and E. coli. Front Microbiol. 2016; 7:198. [DOI:10.3389/fmicb.2016.00198] [PMID] [PMCID] [DOI:10.3389/fmicb.2016.00198]
4. Afshar P, Sedaghat S. Bio-synthesis of silver nanoparticles using water extract of Satureja hortensis L and evaluation of the antibacterial properties. Curr Nanosci. 2016; 12(1): 90-3. [DOI:10.2174/1573413711666150529202238] [DOI:10.2174/1573413711666150529202238]
5. Moradi M, Hassani A, Sefidkon F, Maroofi H. Chemical composition of leaves and flowers essential oil of origanum vulgare ssp. gracile growing wild in Iran. J Essent Oil Bear Plants. 2015; 18(1):242-7. [DOI:10.1080/0972060X.2014.884780] [DOI:10.1080/0972060X.2014.884780]
6. Cheraghali F, Mirmoghtadaie L, Shojaee-Aliabadi S, Hosseini SM. [A comprative study of antimicrobial and antioxidant properties of Walnut Green Husk aqueous extract before and after microencapsulation (Persian)]. Iran J Nutri Sci Food Technol. 2016; 11(2):113-24. [Link]
7. Kozłowska M, Laudy AE, Przybył J, Ziarno M, Majewska E. Chemical composition and antibacterial activity of some medicinal plants from lamiaceae family. Acta Pol Pharm. 2015; 72(4):757-67. [PMID]
8. Argyropoulou C, Akoumianaki-Ioannidou A, Christodoulakis NS, Fasseas C. Leaf anatomy and histochemistry of Lippia citriodora (Verbenaceae). Aust J Bot. 2010; 58(5):398-409. [DOI:10.1071/BT10072] [DOI:10.1071/BT10072]
9. Bahramsoltani R, Rostamiasrabadi P, Shahpiri Z, Marques AM, Rahimi R, Farzaei MH. Aloysia citrodora Paláu (Lemon verbena): A review of phytochemistry and pharmacology. J Ethnopharmacol. 2018; 222:34-51. [DOI:10.1016/j.jep.2018.04.021] [PMID] [DOI:10.1016/j.jep.2018.04.021]
10. Eidi M, Ilchizadeh Kavgani A, Sasaninezhad Z, Ghahramani R, Hamidi Nomani M. [Effect of lippia citriodora on anxiety behaviour in adult male rats (Persian)]. Med J Tabriz Univ Med Sci Health Serv. 2014; 36(5):68-73. [Link]
11. Rezaie MB, Jaymand K. [Study of chemical constituents oil of lippia citriodora H.B. ET K (Persian)]. Pajouhesh Sazandegi. 2002; 53:13-5. [Link]
12. Ombito JO, Salano EN, Yegon PK, Ngetich WK, Mwangi EM. A review on the chemistry of some species of genus Lippia (verbenaceae family). J Sci Innov Res. 2014; 3(4):460-66. [DOI:10.31254/jsir.2014.3411] [DOI:10.31254/jsir.2014.3411]
13. Tabatabaei Yazdi F, Alizade Behbahani B, Heidari Sureshjani M. The comparison of antimicrobial effects of chevil (ferulagoangulata) extract with a variety of common therapeutic antibiotics in vitro. J Arak Univ Med Sci. 2014; 17(3):35-46. [Link]
14. Barrahi M, Esmail A, Elhartiti H, Chahboun N, Benali A, Amiyare R, et al. Chemical composition and evaluation of antibacterial activity of fennel (foeniculum vulgare mill) seed essential oil against some pathogenic bacterial strains. Caspian J Environ Sci. 2020; 18(4):295-307. [DOI: 10.22124/cjes.2020.4276]
15. Alizadeh Behbahani B, Shahidi F, Tabatabaei Yazdi F, Mortazavi SA, Mohebbi M. Antioxidant activity and antimicrobial effect of tarragon (artemisia dracunculus) extract and chemical composition of its essential oil. J Food Me:as char:acter. 2017; 11:847-63. [DOI:10.1007/s11694-016-9456-3] [DOI:10.1007/s11694-016-9456-3]
16. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002; 10(3):178-82.[DOI:10.38212/2224-6614.2748] [DOI:10.38212/2224-6614.2748]
17. Nakajima JI, Tanaka I, Seo S, Yamazaki M, Saito K. LC/PDA/ESI-MS profiling and radical scavenging activity of anthocyanins in various berries. J Biomed Biotechnol. 2004; 2004(5):241-7. [DOI:10.1155/S1110724304404045] [PMID] [PMCID] [DOI:10.1155/S1110724304404045]
18. Maisuthisakul P, Suttajit M, Pongsawatmanit R. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 2007; 100(4):1409-18. [DOI:10.1016/j.foodchem.2005.11.032] [DOI:10.1016/j.foodchem.2005.11.032]
19. Tabatabaei Yazdi F, Alizade-Behbahani B, Mortazavi A. Investigating the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the lavandula stoechas L. and rosmarinus officinalis L. extracts on pathogen bacterias "in vitro". J Paramed Sci. 2014; 5(2):91-101. [Link]
20. Samani Saffari E, Jooyandeh H, Alizadeh Behbahani B. [Evaluation of reciprocal pharmaceutical effect and antimicrobial activity of Shirazi thyme essential oil against some gram-positive and gram-negative bacteria (Persian)]. J Food Sci Technol. 2020; 17(104):1-11. [DOI:10.52547/fsct.17.104.1] [DOI:10.52547/fsct.17.104.1]
21. Gholami A, Arabestani MR, Ahmadi M. [Evaluation of antibacterial activity of aqueous and methanol extracts of allium Jesdianum plant on a number of pathogenic bacteria resistant to antibiotics (Persian)]. Pajouhan Sci J. 2016; 14(4):18-26. [DOI:10.21859/psj-140418] [DOI:10.21859/psj-140418]
22. Barzegar H, Mehrnia MA, Alizadeh Behbahani B. [Determination of the chemical composition, antioxidant activity and the antimicrobial effect of Heracleum Lasiopetalum on infection and food poisoning microorganisms (Persian)]. J Appl Microbiol Food Indust. 2018; 4(4):15-28. [Link]
23. Bajer T, Silha D, Ventura K, Bajerova P. Composition and antimicrobial activity of the essential oil, distilled aromatic water and herbal infusion from Epilobium parviflorum Schreb. Ind Crops Prod. 2017; 100:95-105. [DOI:10.1016/j.indcrop.2017.02.016] [DOI:10.1016/j.indcrop.2017.02.016]
24. Barzegar H, Alizadeh Behbahani B, Mehrnia MA. Quality retention and shelf life extension of fresh beef using Lepidium sativum seed mucilage-based edible coating containing Heracleum lasiopetalum essential oil: An experimental and modeling study. Food Sci Biotechnol. 2019; 29(5):717-28. [DOI:10.1007/s10068-019-00715-4] [PMID] [PMCID] [DOI:10.1007/s10068-019-00715-4]
25. Reyes-Jurado F, Cervantes-Rincón T, Bach H, López-Malo A, Palou E. Antimicrobial activity of Mexican oregano (lippia berlandieri), thyme (thymus vulgaris), and mustard (brassica nigra) essential oils in gaseous phase. Ind Crops Prod. 2019; 131:90-5. [DOI:10.1016/j.indcrop.2019.01.036] [DOI:10.1016/j.indcrop.2019.01.036]
26. Noshad M, Alizadeh Behbahani B, Dehghani S. [Evaluation of the effect of aqueous and ethanolic extraction methods on extraction yield, phenolic compounds, and antioxidant and antimicrobial activity of Stachys schtschegleevii extract (Persian)]. Food Sci Technol. 2020; 17(100):117-25. [DOI:10.52547/fsct.17.100.117] [DOI:10.52547/fsct.17.100.117]
27. Noshad M, Alizadeh Behbahani, B. [Evaluation of the effect of aqueous and ethanolic extraction methods on the antioxidant and antimicrobial characteristics of Lippia citriodora extract (Persian)]. J Food Sci Technol. 2021; 18(118):273-83. [DOI:10.52547/fsct.18.118.273] [DOI:10.52547/fsct.18.118.273]
28. Kumar NK, Kumar KS, Raman BV, Reddy IB, Ramarao M, Rajagopal SV. Antibacterial activity of Lippia citriodora a folklore plant. J Pure Appl Microbiol. 2008; 2(1):249-52. [Link]
29. Aldeen MGN, Mansoor R, AlJoubbeh M. Fluctuations of phenols and flavonoids in infusion of lemon verbena (lippia citriodora) dried leaves during growth stages. Nutr Food Sci. 2015; 45(5):766-73. [DOI:10.1108/NFS-04-2015-0037] [DOI:10.1108/NFS-04-2015-0037]
30. Sosani Gharibvand Z, Alizadeh Behbahani B, Noshad M, Jooyandeh H. [Investigation of the functional groups of bioactive compounds, radical scavenging potential, antimicrobial activity and cytotoxic effect of Callistemon Citrinus aqueous extract on cell line HT29: A laboratory study (Persian)]. J Rafsanjan Univ Med Sci. 19(5):463-84. [DOI:10.29252/jrums.19.5.463] [DOI:10.29252/jrums.19.5.463]
31. Alizadeh Behbahani B, Tabatabaei Yazdi F, Vasiee A, Mortazavi SA. Oliveria decumbens essential oil: Chemical compositions and antimicrobial activity against the growth of some clinical and standard strains causing infection. Microb Pathog. 2018; 114:449-52. [DOI:10.1016/j.micpath.2017.12.033] [PMID] [DOI:10.1016/j.micpath.2017.12.033]
32. Huang WY, Majumder K, Wu J. Oxygen radical absorbance capacity of peptides from egg white protein ovotransferrin and their interaction with phytochemicals. Food Chem. 2010; 123(3):635-41. [DOI:10.1016/j.foodchem.2010.04.083] [DOI:10.1016/j.foodchem.2010.04.083]
33. Prevc T, Segatin N, Ulrih NP, Cigić B. DPPH assay of vegetable oils and model antioxidants in protic and aprotic solvents. Talanta. 2013; 109:13-9. [DOI:10.1016/j.talanta.2013.03.046] [PMID] [DOI:10.1016/j.talanta.2013.03.046]
34. Rahmanzadeh Ishkeh S, Asghari M, Shirzad H, Alirezalu A, Ghasemi G. Lemon verbena (Lippia citrodora) essential oil effects on antioxidant capacity and phytochemical content of raspberry (Rubus ulmifolius subsp. sanctus). Sci Hortic. 2019; 248:297-304. [DOI:10.1016/j.scienta.2018.12.040] [DOI:10.1016/j.scienta.2018.12.040]
35. Roidaki A, Zoumpoulakis PG, Proestos C. Comparison of extraction methods for the determination of antioxidant activity in extracts of hippophae rham noides l. and lippia citriodora. the effect of seasonal collection. Austin J Nutri Food Sci. 2015; 3(1):1-8. [Link]
36. Parveen Z, Nawaz S, Siddique S, Shahzad K. Composition and antimicrobial activity of the essential oil from leaves of curcuma longa L. kasur variety. Indian J Pharm Sci. 2013; 75(1):117-22. [DOI:10.4103/0250-474X.113544] [PMID] [PMCID] [DOI:10.4103/0250-474X.113544]
37. Choupani M, Arabshahi-Delouee S, Alami M. Antioxidant properties of various solvent extracts of lemon verbena (lippia citriodora) leaves. Int J Adv Biol. 2014; 4(2):494-500. [Link]
38. Golmakani MT, Ghassemi A, Eskandari MH, Niakosari M. [Effect of different microwave-extraction methods on active components and antimicrobial activities of lemon verbena essential oils (Persian)]. Res Innov Food Sci Technol. 2016; 5(1):105-18. [DOI:10.22101/JRIFST.2016.06.01.518]
39. Amjad L, Mohammadi Kamalabadi M, Mohammadi Sichani M. [Antibacterial activity of methanol extract of achillea wilhe lmsii C. koch flower and leaf (Persian)]. Qom Univ Med Sci J. 2011; 5(3):50-6. [Link]
40. Negi PS, Jayaprakasha GK, Jena BS. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem. 2003; 80(3):393-7. [DOI:10.1016/S0308-8146(02)00279-0] [DOI:10.1016/S0308-8146(02)00279-0]
41. Fitsiou E, Mitropoulou G, Spyridopoulou K, Vamvakias M, Bardouki H, Galanis A, et al. Chemical composition and evaluation of the biological properties of the essential oil of the dietary phytochemical lippia citriodora. Molecules. 2018; 23(1):123. [DOI:10.3390/molecules23010123] [PMID] [PMCID] [DOI:10.3390/molecules23010123]
42. Mothana RA, Abdo SA, Hasson S, Althawab FM, Alaghbari SA, Lindequist U. Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some yemeni medicinal plants. Evid Based Complement Alternat Med. 2010; 7(3):323-30. [DOI:10.1093/ecam/nen004] [PMID] [PMCID] [DOI:10.1093/ecam/nen004]
43. Sureshjani MH, Yazdi FT, Mortazavi SA, Behbahani BA, Shahidi F. Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria in vitro. Arch Adv Biosci. 2014; 5(2):115-20. [DOI:10.22037/jps.v5i2.5943]
44. Nassiri Semnani S, Ghassempoor N. [Evaluation of the antimicrobial effect of Lemon verbena leaves extracts on Streptococcus pyogenes, Staphylococcus epidermidis, Klebsiella pneumoniae and Brucella melitensis in vitro and animal model study (Persian)]. J Anim Physiol Dev. 2020; 13(4):87-98. [Link]
45. Ahmadi E, Abdollahi A, Najafipour S, Meshkibaf MH, Fasihi-Ramandi M, Namdar N, et al. [Surveying the effect of the phenol compounds on antibacterial activity of herbal extracts : in vitro assessment of herbal extracts in Fasa-Fars province (Persian)]. J Fasa Univ Med Sci. 2016; 6(2):210-20. [Link]
46. Alizadeh M, Kolecka A, Boekhout T, Zarrinfar H, Ghanbari Nahzag M, Badiee P, et al. Identification of candida species isolated from vulvovaginitis in Mashhad, Iran by use of MALDI-TOF MS. Curr Med Mycol. 2017; 3(4):21-5. [DOI:10.29252/cmm.3.4.21] [DOI:10.29252/cmm.3.4.21]
47. Zare Z. Essential oil composition and antimicrobial activity of arial parts of Lippia citriodora H.B.K from Khuzestan at two phases: Before and after blooming. Eco phytochem J Med Plants. 2018; 6(3):110-21. [DOI:10.30495/ejmp.2018.694520]

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