Volume 16, Issue 4 (July 2022)
Qom Univ Med Sci J 2022, 16(4): 280-295 |
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Zohre Jafari1 
, Hassan Bardania2 , Mehrzad Jafari Barmak2 , Yaser Mahmoudi Mourderaz1 , Narges Roustaei3 , Mohammad Sharif Talebianpoor4 , Esmaeel Panahi Kokhdan4 , Seyed Sajjad Khoramrooz * 5
, Hassan Bardania2 , Mehrzad Jafari Barmak2 , Yaser Mahmoudi Mourderaz1 , Narges Roustaei3 , Mohammad Sharif Talebianpoor4 , Esmaeel Panahi Kokhdan4 , Seyed Sajjad Khoramrooz * 5
1- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
2- Cellular and Molecular Research Canter, Yasuj University of Medical Sciences, Yasuj, Iran
3- Department of Epidemiology and Biostatistics, Faculty of Health and Nutrition Sciences, Yasuj University of Medical Sciences, Yasuj, Iran.
4- Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
5- Department of Microbiology, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran. ,khoramrooz@gmail.com
2- Cellular and Molecular Research Canter, Yasuj University of Medical Sciences, Yasuj, Iran
3- Department of Epidemiology and Biostatistics, Faculty of Health and Nutrition Sciences, Yasuj University of Medical Sciences, Yasuj, Iran.
4- Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
5- Department of Microbiology, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran. ,
Abstract: (1267 Views)
Background and Objectives: Regarding the important role of Staphylococcus aureus in burn wound infection, the present study aims to investigate of the effect of Polycaprolactone (PCL) scaffold containing Myrtus communis extract and silver nanoparticles on the healing of burn wound infections induced by Methicillin-Resistant Staphylococcus Aureus (MRSA) in rats.
Methods: In this experimental study, 42 male Wistar rats were divided into seven groups of 6. After anesthesia, second-degree burns on the back of the rats’ necks were created and MRSA was then injected into the wound area. The rats’ wounds were treated daily with nanofiber PCL containing Myrtus communis extract and silver nanoparticles. Antimicrobial properties, wound healing percentage, wound area, malondialdehyde and nitric oxide levels, and total antioxidant capacity were measured. Data were analyzed in GraphPad software using Tukey’s test and ANOVA.
Results: The wound area measurement showed the better effects on would healing in the groups treated by PCL+Myrtus communis extract and PCL+Myrtus communis extract+silver nanoparticles compared other groups. In bacteriological studies, the groups treated by PCL+Myrtus communis extract and PCL+Myrtus communis extract+silver nanoparticle showed more reduced bacterial growth compared to the PCL alone and positive control groups. Moreover, biochemical tests showed a decrease in malondialdehyde and nitric oxide levels in rats treated by PCL+Myrtus communis extract compared to other groups.
Conclusion: Myrtus communis extract and silver nanoparticles can cause changes in PCL properties, which have an important role in reducing the time required for wound healing and in preventing the progression of MRSA-induced wound infection.
Methods: In this experimental study, 42 male Wistar rats were divided into seven groups of 6. After anesthesia, second-degree burns on the back of the rats’ necks were created and MRSA was then injected into the wound area. The rats’ wounds were treated daily with nanofiber PCL containing Myrtus communis extract and silver nanoparticles. Antimicrobial properties, wound healing percentage, wound area, malondialdehyde and nitric oxide levels, and total antioxidant capacity were measured. Data were analyzed in GraphPad software using Tukey’s test and ANOVA.
Results: The wound area measurement showed the better effects on would healing in the groups treated by PCL+Myrtus communis extract and PCL+Myrtus communis extract+silver nanoparticles compared other groups. In bacteriological studies, the groups treated by PCL+Myrtus communis extract and PCL+Myrtus communis extract+silver nanoparticle showed more reduced bacterial growth compared to the PCL alone and positive control groups. Moreover, biochemical tests showed a decrease in malondialdehyde and nitric oxide levels in rats treated by PCL+Myrtus communis extract compared to other groups.
Conclusion: Myrtus communis extract and silver nanoparticles can cause changes in PCL properties, which have an important role in reducing the time required for wound healing and in preventing the progression of MRSA-induced wound infection.
Keywords: Burns, Rats, Polycaprolactone, Methicillin-resistant Staphylococcus aureus, Malondialdehyde
Type of Study: Original Article |
Subject:
میکروب شناسی
Received: 2021/09/12 | Accepted: 2022/04/24 | Published: 2022/04/30
Received: 2021/09/12 | Accepted: 2022/04/24 | Published: 2022/04/30
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