Volume 16, Issue 5 (August 2022)
Qom Univ Med Sci J 2022, 16(5): 414-429 |
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Research code: 15066
Ethics code: IR.MUQ.REC.1400.177
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Norouzi R, Siadatpanah A, Fateh R, Sohrabi F, Adnani Sadati S J. In-vitro Study on the Anti-leishmania Effects of Silver Nanoparticles on Leishmanaia Major Promastigotes. Qom Univ Med Sci J 2022; 16 (5) :414-429
URL: http://journal.muq.ac.ir/article-1-3453-en.html
URL: http://journal.muq.ac.ir/article-1-3453-en.html
Roghayeh Norouzi1 
, Abolghasem Siadatpanah2 , Ruhollah Fateh3 , Fariba Sohrabi3 , Seyed Jafar Adnani Sadati * 4
, Abolghasem Siadatpanah2 , Ruhollah Fateh3 , Fariba Sohrabi3 , Seyed Jafar Adnani Sadati * 4
1- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
2- Department of Nursing, Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Microbiology, Parasitology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
4- Department of Microbiology, Parasitology and Immunology, Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. ,Jafaradnani@yahoo.com
2- Department of Nursing, Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Microbiology, Parasitology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
4- Department of Microbiology, Parasitology and Immunology, Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. ,
Abstract: (1120 Views)
Background and Objectives: Cutaneous leishmaniasis is one of the most important health-threatening diseases in Iran and other countries. Glucantime is currently used to treat this disease, but due to its side effects and high resistance, alternative therapies such as the use of nanoparticles have been considered by researchers. This study aims to investigate the anti-leishmania activity of silver nanoparticles on Leishmania major in vitro.
Methods: This is an experimental study on the anti-leishmania activities of silver nanoparticles at different concentrations of 0.75-0.96 µg/ml after 24, 48 and 72 hours of exposure to 106 live Leishmania major promastigotes. The numbers of live parasites were counted by Trypan Blue on a neobar slide using optical microscope (Hemocytometer method). Glucantime and distilled water were considered as positive and negative controls, respectively. The half-maximal inhibitory concentration (IC50) was calculated by SigmaPlot™ software, version 13. All reactions were done three times and their average was considered as final result.
Results: All concentrations of silver nanoparticles had anti-leishmania activity, where the concentration of 96 µg/ml had the highest effect (100%) 72 hours after exposure. The IC50 was obtained 36.67, 27.2 and 21.08 µg/ml after 24, 48 and 72 hours of exposure, respectively.
Conclusion: Silver nanoparticles have an inhibitory effect on the growth of Leishmania major in different concentrations. However, further in-vivo studies are needed to determine the effictivness of silver nanoparticles.
Methods: This is an experimental study on the anti-leishmania activities of silver nanoparticles at different concentrations of 0.75-0.96 µg/ml after 24, 48 and 72 hours of exposure to 106 live Leishmania major promastigotes. The numbers of live parasites were counted by Trypan Blue on a neobar slide using optical microscope (Hemocytometer method). Glucantime and distilled water were considered as positive and negative controls, respectively. The half-maximal inhibitory concentration (IC50) was calculated by SigmaPlot™ software, version 13. All reactions were done three times and their average was considered as final result.
Results: All concentrations of silver nanoparticles had anti-leishmania activity, where the concentration of 96 µg/ml had the highest effect (100%) 72 hours after exposure. The IC50 was obtained 36.67, 27.2 and 21.08 µg/ml after 24, 48 and 72 hours of exposure, respectively.
Conclusion: Silver nanoparticles have an inhibitory effect on the growth of Leishmania major in different concentrations. However, further in-vivo studies are needed to determine the effictivness of silver nanoparticles.
Type of Study: Original Article |
Subject:
انگل شناسی
Received: 2022/04/24 | Accepted: 2022/07/19 | Published: 2022/08/1
Received: 2022/04/24 | Accepted: 2022/07/19 | Published: 2022/08/1
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