Volume 16, Issue 8 (November 2022)
Qom Univ Med Sci J 2022, 16(8): 664-675 |
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1- Department of Genetics, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran.
2- Department of Biochemistry and Biophysics, College of Biological Sciences, Varamin-Pishva branch, Islamic Azad University, Pishva, Iran. ,ebrahimi@iauvaramin.ac.ir
3- Department of Biology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran.
2- Department of Biochemistry and Biophysics, College of Biological Sciences, Varamin-Pishva branch, Islamic Azad University, Pishva, Iran. ,
3- Department of Biology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran.
Abstract: (407 Views)
Background and Objectives: The liver is exposed to poisoning caused by various chemical compounds. Considering the antioxidant effects found in melon seeds and the role of nuclear factor kappa B (NF-κB) and Myeloperoxidase (MPO) in inflammation caused by oxidative stress, the present study aims to assess the effect of hydroethanolic extract of melon seeds on the transcriptional of NF-κB encoding genes and the MPO gene expression in rats with ethylene glycol-induced toxicity.
Methods: Thirty male rats were randomly divided into five groups of healthy control, poisoned control (0.75% ethylene glycol), and three melon extract-treated poisoned groups (0.75% ethylene glycol and 150, 300, or 600 mg/kg body weight of melon extract) and were treated for 38 days. Then, the liver tissue of the rats was removed to evaluate the transcription of NF-κB encoding genes and the MPO level by real-time PCR method.
Results: The transcription level of NF-κB encoding genes and the MPO level in the poisoned control group rats significantly increased compared to the healthy group. Treatment with hydroethanolic extract of melon seeds at concentrations of 300 and 600 mg/kg body weight caused a significant decrease in the transcription of NF-κB encoding genes. Treatment with hydroethanolic extracts at concentrations of 150, 300, and 600 mg/kg body weight caused a significant decrease in the MPO level compared to the poisoned control group.
Conclusion: The decrease in MPO and NF-κB encoding genes after administration of hydroethanolic extract of melon seeds indicates the therapeutic effect of this extract on liver damage.
Methods: Thirty male rats were randomly divided into five groups of healthy control, poisoned control (0.75% ethylene glycol), and three melon extract-treated poisoned groups (0.75% ethylene glycol and 150, 300, or 600 mg/kg body weight of melon extract) and were treated for 38 days. Then, the liver tissue of the rats was removed to evaluate the transcription of NF-κB encoding genes and the MPO level by real-time PCR method.
Results: The transcription level of NF-κB encoding genes and the MPO level in the poisoned control group rats significantly increased compared to the healthy group. Treatment with hydroethanolic extract of melon seeds at concentrations of 300 and 600 mg/kg body weight caused a significant decrease in the transcription of NF-κB encoding genes. Treatment with hydroethanolic extracts at concentrations of 150, 300, and 600 mg/kg body weight caused a significant decrease in the MPO level compared to the poisoned control group.
Conclusion: The decrease in MPO and NF-κB encoding genes after administration of hydroethanolic extract of melon seeds indicates the therapeutic effect of this extract on liver damage.
Type of Study: Original Article |
Subject:
بیوشیمی بالینی-عمومی
Received: 2022/07/8 | Accepted: 2022/09/26 | Published: 2022/11/1
Received: 2022/07/8 | Accepted: 2022/09/26 | Published: 2022/11/1
References
1. Abbaszadeh S, Nosrati Andevari A, Koohpayeh A, Naghdi N, Alizadeh M, Beyranvand F, et al. Folklore medicinal plants used in liver disease: A review. Int J Green Pharm (IJGP). 2018; 12(3):S463. [Link]
2. Bak J, Je NK, Chung HY, Yokozawa T, Yoon S, Moon JO. Oligonol ameliorates CCl4-induced liver injury in rats via the NF-Kappa B and MAPK signaling pathways. Oxid Med Cell Longev. 2016; 2016:3935841. [DOI:10.1155/2016/3935841] [PMID] [PMCID] [DOI:10.1155/2016/3935841]
3. Gummin DD, Mowry JB, Beuhler MC, Spyker DA, Brooks DE, Dibert KW, et al. 2019 Annual report of the american association of poison control centers' national poison data system (npds): 37th Annual Report. Clin Toxicol (Phila). 2020; 58(12):1360-541. [DOI:10.1080/15563650.2020.1834219] [PMID] [DOI:10.1080/15563650.2020.1834219]
4. Garg U, Lowry J, Algren DA. Ethylene glycol and other glycols.: Analytical and interpretation issues. In: Dasgupta A, editor. Critical issues in alcohol and drugs of abuse testing. Cambridge: Academic Press; 2019. [DOI:10.1016/B978-0-12-815607-0.00005-8] [DOI:10.1016/B978-0-12-815607-0.00005-8]
5. Albensi BC. What is nuclear factor kappa B (NF-κB) doing in and to the mitochondrion? Front Cell Dev Biol. 2019; 7:154. [DOI:10.3389/fcell.2019.00154] [PMID] [PMCID] [DOI:10.3389/fcell.2019.00154]
6. Blazka ME, Beuccoleri A, Simeonova PP, Germolec DR, Pennypacker KR, Luster MI. Acetaminophen-induced hepatotoxicity is associated with early changes in NF-kB and NF-IL6 DNA binding activity. Res Commun Mol Pathol Pharmacol. 1995; 92(3):259-73. [Link]
7. Amanzada A, Malik IA, Nischwitz M, Sultan S, Naz N, Ramadori G. Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver. Histochem Cell Biol. 2011; 135(3):305-15. [DOI:10.1007/s00418-011-0787-1] [PMID] [PMCID] [DOI:10.1007/s00418-011-0787-1]
8. Zargari A. [Medicinal plants (Persian)]. Tehran: Tehran Univercity; 1997.
9. Rolim PM, Fidelis GP, Padilha CEA, Santos ES, Rocha HAO, Macedo GR. Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells. Braz J Med Biol Res. 2018; 51(4):e6069. [DOI:10.1590/1414-431x20176069] [PMID] [PMCID] [DOI:10.1590/1414-431x20176069]
10. Fox ES, Kim JC, Tracy TF. NF-κB activation and modulation in hepatic macrophages during cholestatic injury. J Surg Res. 1997; 72(2):129-34. [DOI:10.1006/jsre.1997.5172] [PMID] [DOI:10.1006/jsre.1997.5172]
11. Hong SH, Seo SH, Lee JH, Choi BT. The aqueous extract from Artemisia capillaris Thunb. inhibits lipopolysaccharide-induced inflammatory response through preventing NF-κB activation in human hepatoma cell line and rat liver. Int J Molecular Med. 2004; 13(5):717-20. [DOI:10.3892/ijmm.13.5.717] [DOI:10.3892/ijmm.13.5.717]
12. Cai H, Song YH, Xia WJ, Jin MW. Aqueous extract of Yin-Chen-Hao decoction, a traditional Chinese prescription, exerts protective effects on concanavalin A-induced hepatitis in mice through inhibition of NF-k B. J Pharm Pharmacol. 2006; 58(5):677-84. [DOI:10.1211/jpp.58.5.0013] [PMID] [DOI:10.1211/jpp.58.5.0013]
13. Wang JY, Guo JS, Li H, Liu SL, Zern MA. Inhibitory effect of glycyrrhizin on NF‐κB binding activity in CC14‐plus ethanol‐induced liver cirrhosis in rats. Liver. 1998; 18(3):180-5. [DOI:10.1111/j.1600-0676.1998.tb00147.x] [PMID] [DOI:10.1111/j.1600-0676.1998.tb00147.x]
14. Zhai KF, Duan H, Cao WG, Gao GZ, Shan LL, Fang XM, et al. Protective effect of Rabdosia amethystoides (Benth) Hara extract on acute liver injury induced by Concanavalin A in mice through inhibition of TLR4-NF-κB signaling pathway. J Pharmacol Sci. 2016; 130(2):94-100. [DOI:10.1016/j.jphs.2015.12.006] [PMID] [DOI:10.1016/j.jphs.2015.12.006]
15. Liu J, Luo D, Wu Y, Gao C, Lin G, Chen J, et al. The protective effect of Sonneratia apetala fruit extract on acetaminophen-induced liver injury in mice. Evid Based Complement Alternat Med. 2019; 2019:6919834. [PMID] [PMCID] [DOI:10.1155/2019/6919834]
16. Lee HC, Yu HP, Liao CC, Chou AH, Liu FC. Escin protects against acetaminophen-induced liver injury in mice via attenuating inflammatory response and inhibiting ERK signaling pathway. Am J Trans Res. 2019; 11(8):5170-82. [PMID]
17. Raish M, Ahmad A, Alkharfy KM, Ahamad SR, Mohsin K, Al-Jenoobi FI, et al. Hepatoprotective activity of Lepidium sativum seeds against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model. BMC Complement Altern Med. 2016; 16(1):501. [PMID] [PMCID] [DOI:10.1186/s12906-016-1483-4]
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