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Isolation, morphology, molecular and differentiation ability of mouse newborn ovarian stem cells assessment
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Samane Adib  |
| Azad university |
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Abstract: (371 Views) |
Background and Objective: The ability of ovarian stem cells to differentiate into oocytes and theca cells may lead to major advances in reproductive biology and infertility treatments. However, little is known about the function, growth and differentiation potential of these immature cells. In this study, the characteristics of adult mouse stem cells and their differentiation potential into osteocyte-like cells and adipocyte-like cells were investigated.
Methods: Mouse newborn ovarian stem cells were isolated and then cultured. RNA extraction and cDNA synthesis were performed. And using PCR, the expression of stem cell markers was checked in these cells. Differentiation potential into osteocyte-like cells and adipocyte-like cells was confirmed by alizarin red and oil red staining, respectively.
Results: The results showed that the isolated ovarian stem cells were adherent after culture and were morphologically similar to fibroblasts cells and had the power to multiply. They expressed markers related to stem cells such as (Nang, Oct4, Sox2), in addition, these cells were able to differentiate into adipocyte and osteocyte like cells 21 days after being placed in the special differentiation medium. In osteocyte -like cells, lipid droplets were observed in these cells with oily red staining.
Conclusion: The findings of this study showed that ovarian stem cells include multipotent stem cells that are able to express markers related to stem cells and differentiate into various cell lines such as osteocyte and adipocyte like cells.
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| Keywords: Ovarian stem cells, oocyte, theca cell, granulosa cell, follicle |
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Type of Study: Original Article |
Subject:
بافت شناسی و جنین شناسی
Received: 2022/08/28 | Accepted: 2022/10/16 | Published: 2022/11/1
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