1- Department of Genetics, Faculty of Biological Sciences, Varamin-Pishva Branch, Islamic Azad university, Pishva, Iran.
2- Department of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran. ,h.zarei@iautmu.ac.ir
3- Department of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Department of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran. ,
3- Department of Biology, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Abstract: (616 Views)
Background and Objectives: Sleep deprivation can increase anxiety by impairing hippocampal-dependent memory and emotional memory and cause changes in mood, mental, cognitive, and motor functions. The NR1-NR2 dimer is the main functional organization structure in any NDMA receptor. This study aims to assess the effect of oral gavage of fish oil on the NR1 and NR2 protein levels in hippocampus of male rats with total sleep deprivation.
Methods: In this study, 25 male Wistar rats were randomly divided into five groups: Control, Sham, sleep deprivation, sham+fish oil, and sleep deprivation+fish oil. At the end of the treatment, the brain of rats was removed and the amount of NR1 and NR2 proteins in the hippocampus was assessed by the Western blotting method.
Results: No significant difference in the level of NR1 and NR2 proteins was observed in the hippocampus of sham group compared to the control group. The expression of these two proteins in the sleep deprivation + fish oil group increased by 62.8% and 83.3%, respectively, compared to the control group, while there was no significant difference in the sleep deprivation group compared to the control group. On the other hand, the levels of these two proteins in the sleep deprivation + fish oil increased by 50 and 116.7%, respectively, compared to the sleep deprivation group.
Conclusion: Fish oil can play a protective and therapeutic role in controlling or relieving the effects of sleep deprivation that can reduce the expression of NR1 and NR2 proteins in other areas of the brain.
Methods: In this study, 25 male Wistar rats were randomly divided into five groups: Control, Sham, sleep deprivation, sham+fish oil, and sleep deprivation+fish oil. At the end of the treatment, the brain of rats was removed and the amount of NR1 and NR2 proteins in the hippocampus was assessed by the Western blotting method.
Results: No significant difference in the level of NR1 and NR2 proteins was observed in the hippocampus of sham group compared to the control group. The expression of these two proteins in the sleep deprivation + fish oil group increased by 62.8% and 83.3%, respectively, compared to the control group, while there was no significant difference in the sleep deprivation group compared to the control group. On the other hand, the levels of these two proteins in the sleep deprivation + fish oil increased by 50 and 116.7%, respectively, compared to the sleep deprivation group.
Conclusion: Fish oil can play a protective and therapeutic role in controlling or relieving the effects of sleep deprivation that can reduce the expression of NR1 and NR2 proteins in other areas of the brain.
Article number: 2762.1
Type of Study: Original Article |
Subject:
ژنتیک
Received: 2022/11/10 | Accepted: 2023/08/16 | Published: 2024/04/29
Received: 2022/11/10 | Accepted: 2023/08/16 | Published: 2024/04/29
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