Akram Mirzaei1 
, Diana Taheri1 , Leila Zareian Baghdadabad1 , Seyed Mohammad Kazem Aghamir Mohammadali * 2
, Diana Taheri1 , Leila Zareian Baghdadabad1 , Seyed Mohammad Kazem Aghamir Mohammadali * 2
1- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
2- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran ,mkaghamir@tums.ac.ir
2- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran ,
Abstract: (623 Views)
Background and purpose: The formation and treatment of kidney stones is one of the most important problems in the medical community and patients and is one of the most common pathological diseases of the urinary tract. In this study, the effects of ethylene glycol and ammonium chloride on the formation of kidney and bladder stones in rats were investigated.
Materials and methods: Renal and bladder stone formation was induced by treating rats with 1% ammonium chloride and 0.75% ethylene glycol in drinking water. The control animals did not receive any treatment. The disease-induced group received ammonium chloride and ethylene glycol in drinking water for 3 and 30 days, respectively. Then, the animals were anesthetized with ether and after a CT scan and confirmation of stone formation in these two organs, their kidneys and bladder were removed and placed in a fixative. Bladder and kidney tissue samples were stained with Hematoxylin and eosin stains for histological studies. To count, calcium oxalate crystal deposits, the slides were examined under a light microscope.
Results: The results of the present study showed that ethylene glycol causes stone formation in both the left and right kidneys and bladder of rats (P≥0.05).
Conclusion: Ethylene glycol with ammonium chloride orally for 30 days is the best model for kidney and bladder stones. Therefore, by creating stones, different therapeutic interventions can be tested in rats.
Materials and methods: Renal and bladder stone formation was induced by treating rats with 1% ammonium chloride and 0.75% ethylene glycol in drinking water. The control animals did not receive any treatment. The disease-induced group received ammonium chloride and ethylene glycol in drinking water for 3 and 30 days, respectively. Then, the animals were anesthetized with ether and after a CT scan and confirmation of stone formation in these two organs, their kidneys and bladder were removed and placed in a fixative. Bladder and kidney tissue samples were stained with Hematoxylin and eosin stains for histological studies. To count, calcium oxalate crystal deposits, the slides were examined under a light microscope.
Results: The results of the present study showed that ethylene glycol causes stone formation in both the left and right kidneys and bladder of rats (P≥0.05).
Conclusion: Ethylene glycol with ammonium chloride orally for 30 days is the best model for kidney and bladder stones. Therefore, by creating stones, different therapeutic interventions can be tested in rats.
Article number: 2917.1
Type of Study: Original Article |
Subject:
نفرولوژی
Received: 2023/06/22 | Accepted: 2023/12/24 | Published: 2024/04/29
Received: 2023/06/22 | Accepted: 2023/12/24 | Published: 2024/04/29
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