Hossein Honari * 
1, Seyed mojtaba Aghaie tabaee zavareh2 , Ashkan Hajinormahamadi2 , Mohammadhassan Samadpour2
1, Seyed mojtaba Aghaie tabaee zavareh2 , Ashkan Hajinormahamadi2 , Mohammadhassan Samadpour2
1- Imam Hossein University , honari.hosein@gmail.com
2- Imam Hossein University
2- Imam Hossein University
Abstract: (306 Views)
Background and objectives The most venomous jellyfish identified is the Chironex fleckeri. Jellyfish stings are a serious threat that has economic and health implications. Nanoparticles have the ability to load a wide range of small medicinal and biological molecules. The small size of nanoparticles, due to the increase in the contact surface with the epithelial surface, has a greater potential for non-specific absorption into the cells. The aim of this study is the expression of C-CfTxB protein in E. coli and loading it in PLGA polymer and investigating its immunogenicity in mice.
Methods The expression of C-cftxB gene was induced by IPTG and the recombinant protein was confirmed by western blot technique. After protein purification, antigen was loaded in PLGA polymer. The size and zeta potential of nanoparticles containing protein were measured. Prepared Nano antigen, protein with adjuvant; PLGA and PBS nanoparticles were injected into the mice groups in two and four times. The amount of antibody in their serum was measured by ELISA test. At the end, mice were challenged with Rhopilema nomadica jellyfish venom.
Results The size and zeta potential of nanoparticles containing protein were 200 nm and -20.2 mV, respectively. The photo-electron microscope showed that the nanoparticles were spherical. The results of the challenge test showed that the immunized mice tolerated 50 times the LD50 of jellyfish venom after 60 days.
Conclusions Considering the lack of cardiotoxicity and neurotoxicity of C-CfTXB recombinant protein in mice, this protein can be considered as a vaccine candidate against jellyfish venom
Methods The expression of C-cftxB gene was induced by IPTG and the recombinant protein was confirmed by western blot technique. After protein purification, antigen was loaded in PLGA polymer. The size and zeta potential of nanoparticles containing protein were measured. Prepared Nano antigen, protein with adjuvant; PLGA and PBS nanoparticles were injected into the mice groups in two and four times. The amount of antibody in their serum was measured by ELISA test. At the end, mice were challenged with Rhopilema nomadica jellyfish venom.
Results The size and zeta potential of nanoparticles containing protein were 200 nm and -20.2 mV, respectively. The photo-electron microscope showed that the nanoparticles were spherical. The results of the challenge test showed that the immunized mice tolerated 50 times the LD50 of jellyfish venom after 60 days.
Conclusions Considering the lack of cardiotoxicity and neurotoxicity of C-CfTXB recombinant protein in mice, this protein can be considered as a vaccine candidate against jellyfish venom
Article number: 1360.3
Keywords: Vaccine candidate, Jellyfish venom, Recombinant protein, Nano encapsulation, Antigenicity.
Type of Study: Original Article |
Subject:
ایمونولوژی
Received: 2023/10/28 | Accepted: 2023/12/5 | Published: 2024/04/29
Received: 2023/10/28 | Accepted: 2023/12/5 | Published: 2024/04/29
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