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:: Volume 10, Issue 1 (March 2016 2016) ::
Qom Univ Med Sci J 2016, 10(1): 48-55 Back to browse issues page
Detection of Exotoxin A, Y, T, U, S genes of Pseudomonas aeruginosa Isolates Resistant to Third-Generation Cephalosporins in Clinical Samples of Hospitalized Patients in Hospitals of Qom City, Iran
Seyyed Soheil Aghaei 1, Ali Javadi 2, Yaser Sharifi 3, Abbas Morovvati 2
1- Islamic Azad University , soheilaghaee@yahoo.com
2- Islamic Azad University
3- Young Researchers & Elite Club, Qom
Abstract:   (3122 Views)

Background and Objectives: One of the most common problems in hospitals is infections caused by Pseudomonas aeruginosa that is an opportunistic pathogen for humans. Five important enzymes, including exotoxin A, Y, T, U, S are major virulence factors in this bacterium. The purpose of this study was to determine the frequency of these enzymes in the samples isolated from hospitals of Qom city and to determine their antibiogram pattern against third-generation cephalosporins using CLSI standards.

Methods: In this cross-sectional analytical study, 120 strains of P. aeruginosa isolated from various clinical specimens from hospitals of Qom city, were examined. At first, bacteria were identified using biochemical tests, and then their antibiogram pattern against third-generation cephalosporins was obtained according to CLSI standards. After genome extraction (extraction kit of Sinaclon Co.), primers design and evaluation were performed through NCBI website, and PCR reaction was conducted to evaluate each gene. After electrophoresis, the bands’ results obtained from PCR reaction, was observed in gel doc system.

Results: The frequency of the isolates in the samples of skin, blood, lung, and urine was, respectively, 63.2, 16.1, 16.1/, and 4.1%. The frequency of exotoxins A, T, U, S, and exoenzyme Y were respectively obtained to be 100, 37.9, 26.4, 39.1, and 29.9%.

Conclusion: According to the results of this study, drug resistance and presence of virulence factors, such as exotoxins can be a serious warning to treatment centers in terms of control of disease caused by this bacterium.

Keywords: Pseudomonas aeruginosa, Exotoxins, Drug Resistance, Qom, Iran
Full-Text [PDF 544 kb]   (2040 Downloads)    
Type of Study: Original Article |
Received: 2016/03/14 | Accepted: 2016/03/14 | Published: 2016/03/14
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Aghaei S S, Javadi A, Sharifi Y, Morovvati A. Detection of Exotoxin A, Y, T, U, S genes of Pseudomonas aeruginosa Isolates Resistant to Third-Generation Cephalosporins in Clinical Samples of Hospitalized Patients in Hospitals of Qom City, Iran. Qom Univ Med Sci J . 2016; 10 (1) :48-55
URL: http://journal.muq.ac.ir/article-1-448-en.html
Volume 10, Issue 1 (March 2016 2016) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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