Volume 12, Issue 3 (May 2018)
Qom Univ Med Sci J 2018, 12(3): 9-18 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mirzaie A, Piroozmand S, Mousavi Z, Rustaiyan A. Investigation of Chemical Composition of Helichrysum artemisioides Essential Oil, and its Antibacterial and Cytotoxic Effects on Colon Cancer Cell Line and Analysis of Apoptotic Gene Expression Using PCR Method. Qom Univ Med Sci J 2018; 12 (3) :9-18
URL: http://journal.muq.ac.ir/article-1-1333-en.html
URL: http://journal.muq.ac.ir/article-1-1333-en.html
1- Department of Pharmacology & Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
2- Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran.
3- Department of Chemistry, Science & Research Branch, Islamic Azad University, Tehran, Iran. ,a.rustaiyan@srbiau.ac.ir
2- Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran.
3- Department of Chemistry, Science & Research Branch, Islamic Azad University, Tehran, Iran. ,
Abstract: (7832 Views)
Background and Objectives: Helichrysum artemisioides is one of the endemic medicinal plants in Iran. The aim of this research was to identify the chemical composition of H. artemisioides essential oil and its antibacterial and cytotoxic effects on colon cancer cell line (HT29).
Methods: In this study, the essential oil of H. artemisioides was collected by hydrodistillation method using Clevenger apparatus. Chemical composition of essential oil was identified using a GC-MS device. Subsequently, antibacterial effect of the essential oil on pathogenic bacteria and its cytotoxic activity on colon cancer cell line (HT29), were determined using MIC and MTT methods, respectively. The expression of Bax and Bcl2 apoptotic genes in HT29 cell line, was assessed using real time PCR method.
Results: GC-MS analysis of essential oil of the plant showed 55 compounds, most of which belonged to Carvacrol (12.5%) and 1R-α-Pinene (12.5%). Furthermore, the essential oil of this plant had the highest effect on Gram-positive bacteria and the least effect on gram-negative bacteria. Moreover, the essential oil of this plant had IC50=31.54mg/ml on HT29 cell line. Results of real time PCR showed increase in expression level of Bax gene and decrease in expression level of Bcl2, respectively, 2.9 ± 0.2 (p<0.05) and 0.2±0.7 (p<0.05).
Conclusion: Considering antibacterial and anticancer effects of H. artemisioides essential oil, it appears that this plant has the potential to be used in pharmaceutical industries.
Methods: In this study, the essential oil of H. artemisioides was collected by hydrodistillation method using Clevenger apparatus. Chemical composition of essential oil was identified using a GC-MS device. Subsequently, antibacterial effect of the essential oil on pathogenic bacteria and its cytotoxic activity on colon cancer cell line (HT29), were determined using MIC and MTT methods, respectively. The expression of Bax and Bcl2 apoptotic genes in HT29 cell line, was assessed using real time PCR method.
Results: GC-MS analysis of essential oil of the plant showed 55 compounds, most of which belonged to Carvacrol (12.5%) and 1R-α-Pinene (12.5%). Furthermore, the essential oil of this plant had the highest effect on Gram-positive bacteria and the least effect on gram-negative bacteria. Moreover, the essential oil of this plant had IC50=31.54mg/ml on HT29 cell line. Results of real time PCR showed increase in expression level of Bax gene and decrease in expression level of Bcl2, respectively, 2.9 ± 0.2 (p<0.05) and 0.2±0.7 (p<0.05).
Conclusion: Considering antibacterial and anticancer effects of H. artemisioides essential oil, it appears that this plant has the potential to be used in pharmaceutical industries.
Keywords: Helichrysum artemisioides, Colon cancer, Antibacterial agents, Apoptosis, Real-time polymerase chain reaction.
Type of Study: Original Article |
Subject:
سلولی و مولکولی
Received: 2016/12/2 | Accepted: 2017/02/12 | Published: 2018/05/17
Received: 2016/12/2 | Accepted: 2017/02/12 | Published: 2018/05/17
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Owned by Qom University of Medical Sciences
Published by Qom University of Medical Sciences Press
eISSN: 2008-1375
pISSN: 1735-7799
Email: journal[at]muq.ac.ir
j.mua.ac[at]gmail.com
