Volume 12, Issue 9 (Novamber 2018)
Qom Univ Med Sci J 2018, 12(9): 38-46 |
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Tabatabaee M, Ahmadi R, Kassaee ُ M. The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression . Qom Univ Med Sci J 2018; 12 (9) :38-46
URL: http://journal.muq.ac.ir/article-1-2082-en.html
URL: http://journal.muq.ac.ir/article-1-2082-en.html
1- Department of Physiology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University , Mohadeseh@yahoo.com
2- Islamic Azad University
3- Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University
2- Islamic Azad University
3- Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University
Abstract: (4323 Views)
Background and Objectives: Studies have shown that Scrophularia striata has various treatment effects. For this purpose, in this study, the cytotoxic effect of hydroalcoholic extract of aerial parts of Scrophularia striata was investigated on MCF-7 cell line, and BAX and KAI1 genes expression was assessed.
Methods: In this laboratory experimental study, at first, extract of the plant was prepared using maceration method. The effect of different concentrations of the extract (0.001, 0.001, 0.1, 1, and 10mg/ml) was investigated on the viability of MCF-7 cells using MTT assay method. Also, the effects of doses of 1, 0.1, and 0.01mg/ml of the extract, was evaluated on BAX and KAI1 genes using real-time PCR. The data were analyzed using one way AN0VA test.
Results:.In this study, all concentrations of the extract caused a significant decrease in viability of MCF7 cells compared to the control group (p<0.001). On the one hand, the relative expression level of antimetastasis KAI1 gene and BAX apoptotic gene significantly decreased in the groups that received 0.1 and 1 mg/ml of extract compared to the control group (p<0.001).
Conclusion: The findings of this study showed that hydroalcoholic extract of aerial parts of Scrophularia striata has cytotoxic effects on breast cancer cells, which, in this regard, can induce of BAX gene dependent apoptosis. Moreover, this extract has antimetastatic effects on breast cancer cells through increasing the relative expression level of antimetastasis KAI1 gene.
Methods: In this laboratory experimental study, at first, extract of the plant was prepared using maceration method. The effect of different concentrations of the extract (0.001, 0.001, 0.1, 1, and 10mg/ml) was investigated on the viability of MCF-7 cells using MTT assay method. Also, the effects of doses of 1, 0.1, and 0.01mg/ml of the extract, was evaluated on BAX and KAI1 genes using real-time PCR. The data were analyzed using one way AN0VA test.
Results:.In this study, all concentrations of the extract caused a significant decrease in viability of MCF7 cells compared to the control group (p<0.001). On the one hand, the relative expression level of antimetastasis KAI1 gene and BAX apoptotic gene significantly decreased in the groups that received 0.1 and 1 mg/ml of extract compared to the control group (p<0.001).
Conclusion: The findings of this study showed that hydroalcoholic extract of aerial parts of Scrophularia striata has cytotoxic effects on breast cancer cells, which, in this regard, can induce of BAX gene dependent apoptosis. Moreover, this extract has antimetastatic effects on breast cancer cells through increasing the relative expression level of antimetastasis KAI1 gene.
Type of Study: Original Article |
Subject:
فیزیولوژی
Received: 2018/03/17 | Accepted: 2018/06/18 | Published: 2018/12/15
Received: 2018/03/17 | Accepted: 2018/06/18 | Published: 2018/12/15
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