Volume 14, Issue 1 (March 2020)
Qom Univ Med Sci J 2020, 14(1): 38-50 |
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Azizpour A. A Study of Salmonella Spp. Contamination Rate of Eggs and Assessment of their Antibiotic Resistance Pattern in Ardabil, Iran. Qom Univ Med Sci J 2020; 14 (1) :38-50
URL: http://journal.muq.ac.ir/article-1-2740-en.html
URL: http://journal.muq.ac.ir/article-1-2740-en.html
Department of Medicinal Plants, Meshginshahr School of Agriculture, University of Mohaghegh Ardabili , Aidin_azizpour@uma.ac.ir
Abstract: (4559 Views)
Background and Objectives: Salmonellosis is one of the most important diseases among humans and poultry worldwide. Contaminated eggs are important sources of salmonellosis outbreak in humans. The aim of this research was to investigate the rate of contamination of eggs with Salmonella spp. and to evaluate their drug resistance pattern in Ardabil city.
Methods: A total of 110 egg samples (37 native eggs, 41 bulk eggs, and 32 labeled industrial eggs), were collected randomly from retail stores in different parts of Ardabil city during the second half of the year 2019. Shell and contents of the eggs, were evaluated for Salmonella contamination using standard culture methods, and Salmonella serotyping and PCR assay were used for definite diagnosis of salmonella isolates. standard disk diffusion method was used to determine antimicrobial resistance of the isolates against 15 antimicrobial agents.
Results: Out of 110 egg samples examined, 4 eggs (3.63%), were infected with Salmonella. Shell contamination of native eggs, bulk eggs, and industrial eggs with Salmonella were 5.40%, 2.43%, and 0%, respectively. Salmonella contamination in the contents of the native eggs was 2.94%, while no contamination was observed in the contents of bulk eggs and industrial eggs. S. typhimurium was detected by serotyping and PCR technique on positive samples. Among the 15 antibiotics studied, the highest resistance was observed to streptomycin, tetracycline, chloramphenicol, and nalidixic acid with 100% and the lowest resistance was to ceftazidime and florfenicol with 40% and nitrofurantoin with 20%.
Conclusion: The findings of this study were indicative of the presence of Salmonella contamination in native eggs and bulk eggs compared to the industrial eggs, thus it is of great importance to observe the hygienic points, including proper and adequate cooking of eggs. Also, excessive use of antibiotics in poultry farms should be avoided to prevent the spread of resistant bacteria.
Methods: A total of 110 egg samples (37 native eggs, 41 bulk eggs, and 32 labeled industrial eggs), were collected randomly from retail stores in different parts of Ardabil city during the second half of the year 2019. Shell and contents of the eggs, were evaluated for Salmonella contamination using standard culture methods, and Salmonella serotyping and PCR assay were used for definite diagnosis of salmonella isolates. standard disk diffusion method was used to determine antimicrobial resistance of the isolates against 15 antimicrobial agents.
Results: Out of 110 egg samples examined, 4 eggs (3.63%), were infected with Salmonella. Shell contamination of native eggs, bulk eggs, and industrial eggs with Salmonella were 5.40%, 2.43%, and 0%, respectively. Salmonella contamination in the contents of the native eggs was 2.94%, while no contamination was observed in the contents of bulk eggs and industrial eggs. S. typhimurium was detected by serotyping and PCR technique on positive samples. Among the 15 antibiotics studied, the highest resistance was observed to streptomycin, tetracycline, chloramphenicol, and nalidixic acid with 100% and the lowest resistance was to ceftazidime and florfenicol with 40% and nitrofurantoin with 20%.
Conclusion: The findings of this study were indicative of the presence of Salmonella contamination in native eggs and bulk eggs compared to the industrial eggs, thus it is of great importance to observe the hygienic points, including proper and adequate cooking of eggs. Also, excessive use of antibiotics in poultry farms should be avoided to prevent the spread of resistant bacteria.
Type of Study: Original Article |
Subject:
میکروب شناسی
Received: 2020/02/9 | Accepted: 2020/04/21 | Published: 2020/03/29
Received: 2020/02/9 | Accepted: 2020/04/21 | Published: 2020/03/29
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