Volume 15, Issue 8 (November 2021)                   Qom Univ Med Sci J 2021, 15(8): 550-563 | Back to browse issues page

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Rostami E, Heidari R, Shirzad ‪, Motevaseli E, Mazlumi M A. Comparison of the Effects of Native and Commercial Probiotic on Gluten Degradation. Qom Univ Med Sci J 2021; 15 (8) :550-563
URL: http://journal.muq.ac.ir/article-1-3050-en.html
Comparison of the Effects of Native and Commercial Probiotic on Gluten Degradation
Elham Rostami1 , Reza Heidari2 , ‪Mahdieh Shirzad3 , Elahe Motevaseli4 , Mohammad Ali Mazlumi * 5
1- Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2- Research Center for Cancer Screening and Epidemiology, AJA University of Medical Sciences, Tehran, Iran.
3- Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, University of Tehran, College of Science, Tehran, Iran.
4- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
5- Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. , m-mazlomi@tums.ac.ir
Abstract:   (1426 Views)
Background and Objectives: The main treatment for celiac disease is a gluten-free diet. Gluten infection occurs in 55%-32% of these patients, which can cause symptoms in these patients. Many patients with celiac disease are dissatisfied with a gluten-free diet and are interested in a non-dietary alternative treatment.
Methods: In this study, probiotic samples of local dairy (milk, yogurt, and cheese) from Iran were collected in sterile conditions and after enzymatic extraction using a lysis buffer and a sonication method for enzyme activity of prolyl endopeptidase and aminopeptidase N were evaluated.
Results: The highest absorption rate for Z-Gly-Pro-4-nitroanilide substrate that performs the evaluation of prolyl endopeptidase enzyme, respectively, for strains C10-1 (L. pentosus), B6-1 (L. fermentum), C5-2 (L.paracasei), Y5 (L.paracasei) and C11-1 (L. pentosus) and in relation to Leu-p-NA which assesses aminopeptidase N The highest uptake was observed for strains K1 (L. helveticus), B8-2 (L. plantarum), Y5 (L.paracasei), P2-3 and P35 (L. plantarum), respectively. All of the above strains showed significantly different uptake than the negative control containing buffer and substrate (P<0.05). Finally, the 16s rRNA sequence of the isolated strains was registered in NCBI database.
Conclusion: The results of the present study showed that number of native Iranian probiotics have effective activity of prolyl endopeptidase and aminopeptidase N which can be used as a selective combination for gluten degradation and to help celiac patients.
Keywords: Probiotics, Glutens, Celiac disease, Prolyl oligopeptidases, Aminopeptidase N
Full-Text [PDF 5162 kb]   (487 Downloads)    
Type of Study: Original Article | Subject: تغذیه
Received: 2021/01/31 | Accepted: 2021/10/23 | Published: 2021/11/1
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