Volume 15, Issue 6 (September 2021)
Qom Univ Med Sci J 2021, 15(6): 426-433 |
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Zamani-Garmsiri F, Najafi M, Mohammadi A, Shaikhnia F, Ghasempour G. Evaluation of the toxicity effects of polyethyleneimine (PEI) on cell viability and morphology of VSMC cells. Qom Univ Med Sci J 2021; 15 (6) :426-433
URL: http://journal.muq.ac.ir/article-1-3145-en.html
URL: http://journal.muq.ac.ir/article-1-3145-en.html
Fahimeh Zamani-Garmsiri1 
, Mohammad Najafi2 , Asghar Mohammadi3 , Farhad Shaikhnia2 , Ghasem Ghasempour * 4
, Mohammad Najafi2 , Asghar Mohammadi3 , Farhad Shaikhnia2 , Ghasem Ghasempour * 4
1- Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2- Department of Clinical Biochemistry, Faculty of Medical, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
4- Department of Clinical Biochemistry, Faculty of Medical, Iran University of Medical Sciences, Tehran, Iran. ,ghasempourghasem66@gmail.com
2- Department of Clinical Biochemistry, Faculty of Medical, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
4- Department of Clinical Biochemistry, Faculty of Medical, Iran University of Medical Sciences, Tehran, Iran. ,
Abstract: (1619 Views)
Background and Objectives: Today, PEI is used for high-efficiency gene transfection. This polycation can have toxic effects on cells due to charged amine groups. This study evaluated the toxic effects of PEI in both single and oligonucleotide-binding modes on VSMC cells.
Methods: First, VSMC cells were cultured and then transfected by nanoparticles in two PEI states alone and containing oligonucleotides. After 24 hours, cell viability and morphology were assessed using MTT and electron microscopy methods respectively. Data were analysed using the one-way ANOVA, followed by the Tukey Post-Hoc test.
Results: The results showed that nanoparticles significantly reduced the cell viability by causing toxicity to cells (P<0.0001) and also its images showed cell death and apoptosis. But PEI containing oligonucleotides had no toxic effects on cell viability (P= 0.7414). Its electron microscope image also showed a normal cell morphology.
Conclusion: Overall, this study showed that the effect of PEI toxicity on VSMC cells is inhibited by oligonucleotide sequencing and thus neutralizing the positive charges of the amine group.
Methods: First, VSMC cells were cultured and then transfected by nanoparticles in two PEI states alone and containing oligonucleotides. After 24 hours, cell viability and morphology were assessed using MTT and electron microscopy methods respectively. Data were analysed using the one-way ANOVA, followed by the Tukey Post-Hoc test.
Results: The results showed that nanoparticles significantly reduced the cell viability by causing toxicity to cells (P<0.0001) and also its images showed cell death and apoptosis. But PEI containing oligonucleotides had no toxic effects on cell viability (P= 0.7414). Its electron microscope image also showed a normal cell morphology.
Conclusion: Overall, this study showed that the effect of PEI toxicity on VSMC cells is inhibited by oligonucleotide sequencing and thus neutralizing the positive charges of the amine group.
Type of Study: Original Article |
Subject:
علوم پایه
Received: 2021/05/20 | Accepted: 2021/09/1 | Published: 2021/09/1
Received: 2021/05/20 | Accepted: 2021/09/1 | Published: 2021/09/1
References
1. Pishavar E, Shafiei M, Mehri S, Ramezani M, Abnous K. The effects of polyethylenimine/DNA nanoparticle on transcript levels of apoptosis-related genes. Drug Chem Toxicol. 2017; 40(4):406-9. [PMID] [DOI:10.1080/01480545.2016.1245318]
2. Craciun BF, Gavril G, Peptanariu D, Ursu LE, Clima L, Pinteala M. Synergistic effect of low molecular weight polyethylenimine and polyethylene glycol components in dynamic nonviral vector structure, toxicity, and transfection efficiency. Molecules. 2019; 24(8):1460. [DOI:10.3390/molecules24081460] [PMID] [PMCID] [DOI:10.3390/molecules24081460]
3. Vancha AR, Govindaraju S, Parsa KV, Jasti M, González-García M, Ballestero RP. Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer. BMC Biotechnol. 2004; 4:23. [DOI:10.1186/1472-6750-4-23] [PMID] [PMCID] [DOI:10.1186/1472-6750-4-23]
4. Kuo WT, Huang HY, Huang YY. Intracellular trafficking, metabolism and toxicity of current gene carriers. Curr Drug Metab. 2009; 10(8):885-94. [PMID] [DOI:10.2174/138920009790274504]
5. Neuberg P, Kichler A. Recent developments in nucleic acid delivery with polyethylenimines. Adv Genet. 2014; 88:263-88. [DOI:10.1016/B978-0-12-800148-6.00009-2] [PMID] [DOI:10.1016/B978-0-12-800148-6.00009-2]
6. Lv H, Zhang S, Wang B, Cui S, Yan J. Toxicity of cationic lipids and cationic polymers in gene delivery. J Control Release. 2006; 114(1):100-9. [DOI:10.1016/j.jconrel.2006.04.014] [PMID] [DOI:10.1016/j.jconrel.2006.04.014]
7. Godbey WT, Wu KK, Mikos AG. Poly (ethylenimine) and its role in gene delivery. J Control Release. 1999; 60(2-3):149-60. [DOI:10.1016/S0168-3659(99)00090-5] [DOI:10.1016/S0168-3659(99)00090-5]
8. Dabbaghi M, Kazemi Oskuee R, Hashemi K, Afkhami Goli A. Evaluating polyethyleneimine/DNA nanoparticles-mediated damage to cellular organelles using endoplasmic reticulum stress profile. Artif Cells Nanomed Biotechnol. 2018; 46(1):192-9. [PMID] [DOI:10.1080/21691401.2017.1304406]
9. Valente JFA, Pereira P, Sousa A, Queiroz JA, Sousa F. Effect of plasmid DNA size on chitosan or polyethyleneimine polyplexes formulation. Polymers. 2021; 13(5):793. [DOI:10.3390/polym13050793] [PMID] [PMCID] [DOI:10.3390/polym13050793]
10. Ghasempour G, Mohammadi A, Zamani-Garmsiri F, Najafi M. miRNAs through β-ARR2/p-ERK1/2 pathway regulate the VSMC proliferation and migration. Life Sci. 2021; 279:119703. [DOI:10.1016/j.lfs.2021.119703] [PMID] [DOI:10.1016/j.lfs.2021.119703]
11. Ghasempour G, Mahabadi VP, Shabani M, Mohammadi A, Zamani-Garmsiri F, Amirfarhangi A, et al. miR-181b and miR-204 suppress the VSMC proliferation and migration by downregulation of HCK. Microvasc Res. 2021; 136:104172. [DOI:10.1016/j.mvr.2021.104172] [PMID] [DOI:10.1016/j.mvr.2021.104172]
12. Kumar P, Nagarajan A, Uchil PD. Analysis of cell viability by the MTT assay. Cold Spring Harb Protoc. 2018; 2018(6). [DOI:10.1101/pdb.prot095505] [PMID] [DOI:10.1101/pdb.prot095505]
13. Hao F, Li Y, Zhu J, Sun J, Marshall B, Lee RJ, et al. Polyethylenimine-based formulations for delivery of oligonucleotides. Curr Med Chem. 2019; 26(13):2264-84. [PMID] [DOI:10.2174/0929867325666181031094759]
14. Florea BI, Meaney C, Junginger HE, Borchard G. Transfection efficiency and toxicity of polyethylenimine in differentiated Calu-3 and nondifferentiated COS-1 cell cultures. AAPS PharmSci. 2002; 4(3):E12. [DOI:10.1208/ps040312] [PMID] [PMCID] [DOI:10.1208/ps040312]
15. Wang M, Lu P, Wu B, Tucker JD, Cloer C, Lu Q. High efficiency and low toxicity of polyethyleneimine modified pluronics (PEI-pluronic) as gene delivery carriers in cell culture and dystrophic mdx mice. J Mater Chem. 2012; 22(13):6038-46. [DOI:10.1039/c2jm15625c] [DOI:10.1039/c2jm15625c]
16. Rezaee M, Gholami L, Gildeh MS, Ramezani M, Oskuee RK. Charge reduction: An efficient strategy to reduce toxicity and increase the transfection efficiency of high molecular weight polyethylenimine. J Pharm Investig. 2019; 49(1):105-14. [DOI:10.1007/s40005-018-0388-2] [DOI:10.1007/s40005-018-0388-2]
17. Thomas M, Klibanov AM. Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cells. Proc Natl Acad Sci U S A. 2002; 99(23):14640-5. [DOI:10.1073/pnas.192581499] [PMID] [PMCID] [DOI:10.1073/pnas.192581499]
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