Volume 15, Issue 5 (August 2021)                   Qom Univ Med Sci J 2021, 15(5): 368-377 | Back to browse issues page

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Khazaei S, Soleimani M, Ahmaditafti S H, Hojati Z. Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold. Qom Univ Med Sci J 2021; 15 (5) :368-377
URL: http://journal.muq.ac.ir/article-1-3193-en.html
Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
Samaneh Khazaei1 , Masoud Soleimani2 , Seyyed hossein Ahmaditafti3 , Zohreh Hojati * 4
1- Department of Cell and Molecular Biology, School of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
2- Department of Tissue Engineering and Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
4- Department of Tissue Engineering and Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran , z.hojati@sci.ui.ac.ir
Abstract:   (1824 Views)
Background and Objectives: Cardiovascular disease is one of the leading causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources of cell-based therapies in heart regeneration. There are several approaches to differentiate MSCs into cardiac-like cells, such as genetic modification. In addition, using of 3D culture, such as hydrogels, increases the efficiency of differentiation.
Methods: In the present study, lentiviruses containing microRNA 1 (miR- 1) and myocardium (Myocd) were co-transducted to mouse adipose-derived MSCs. Three days after, transduced MSCs were transferred to a hydrogel containing chitosan and collagen. After 21 days, the differentiation of encapsulated cells was evaluated. In this regard, the expression of cardiac markers such as NK2 homeobox 5 (Nkx2-5), GATA binding protein 4 (Gata4) and troponin T type 2 (Tnnt2) at the level of gene and protein were investigated.
Results: The results of real-time quantitative polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by transfer to composite hydrogel increased the expression of cardiac markers.
Conclusion: The use of 3D culture such as chitosan/collagen hydrogel improves the differentiation of MSCs and subsequently obtains more mature cells for use in cell-based regenerative medicine
Keywords: Cardiovascular diseases, Mesenchymal stem cells, miR-1, Organ culture techniques, Cell differentiation
Full-Text [PDF 7223 kb]   (559 Downloads)    
Type of Study: Original Article | Subject: سلولی و مولکولی
Received: 2021/07/11 | Accepted: 2021/08/9 | Published: 2021/08/1
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