Volume 15, Issue 9 (December 2021)
Qom Univ Med Sci J 2021, 15(9): 590-597 |
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Norouzi R, Siadatpanah A, Mirzaei F, Fateh R, Khalifeh Gholi M, Adnani Sadati S J. Evaluation of Antileishmania Effect of Methanolic Extract of Dandelion Root (Taraxacum Officinale) on Leishmanaia Major Promastigotes in Vitro Techniques. Qom Univ Med Sci J 2021; 15 (9) :590-597
URL: http://journal.muq.ac.ir/article-1-3257-en.html
URL: http://journal.muq.ac.ir/article-1-3257-en.html
Roghayeh Norouzi1 
, Abolghasem Siadatpanah2 , Farzaneh Mirzaei3 , Ruhollah Fateh4 , Mohammad Khalifeh Gholi4 , Seyyed Jafar Adnani Sadati * 5
, Abolghasem Siadatpanah2 , Farzaneh Mirzaei3 , Ruhollah Fateh4 , Mohammad Khalifeh Gholi4 , Seyyed Jafar Adnani Sadati * 5
1- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
2- Department of Health Information Technology, School of Ferdows Paramedical, Birjand University of Medical Sciences,Birjand, Iran.
3- Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Microbiology Immunology and Parasitology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
5- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. ,Jafaradnani@yahoo.com- Jadnani@muq.ac.ir
2- Department of Health Information Technology, School of Ferdows Paramedical, Birjand University of Medical Sciences,Birjand, Iran.
3- Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Department of Microbiology Immunology and Parasitology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
5- Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. ,
Abstract: (1813 Views)
Background and Objectives: Cutaneous leishmaniasis is one of the common diseases between humans and animals that is considered a health problem in Iran and many countries around the world. Currently, glucantime is used to treat cutaneous leishmaniasis. Due to its high side effects and resistance, the use of alternative therapies, especially plants and natural compounds, has been highly recommended by researchers. The aim of this study is to investigate the effect of the methanolic extract of dandelion root (Taraxacum officinale) on Leishmania major in vitro techniques.
Methods: In this experimental study, the extract was prepared by the Soxhlet method and to evaluate the effect of methanolic extract of dandelion root on L. major parasite at concentrations of 300-1200 mg/ml. Amphotericin B and distilled water were considered as positive and negative controls, respectively. Then, 106 live parasites were added to all tubes, and all groups were kept at 25 ±1 °C. At 24, 48, and 72 hours, the number of live parasites was counted by Trypan Blue using a neobar slide and light microscope (Hemocytometer method). Then Half-maximal inhibitory concentration (IC50) value for the above extract was calculated using SigmaPlot™ 13 software. All steps of the experiment were done in triplicate and the results were considered as average.
Results: The IC50 content of methanolic extract of dandelion roots after 24, 48, and 72 hours on L. major were calculated to be 1.04, 0.9, and 0.68 µg/ml. The highest growth inhibition (100%) was observed at a concentration of 1200 μg/ml after 72 hours of exposure. There was a significant difference between the IC50 extract and amphotericin B drug after 24, 48 and 72 hours (P<0.05).
Conclusion: Methanolic extract of dandelion root in different concentrations has an inhibitory effect on the growth of L. major and it is suggested that more and more complete studies be performed on the components of this plant and the lethal effect of the parasite in vivo.
Methods: In this experimental study, the extract was prepared by the Soxhlet method and to evaluate the effect of methanolic extract of dandelion root on L. major parasite at concentrations of 300-1200 mg/ml. Amphotericin B and distilled water were considered as positive and negative controls, respectively. Then, 106 live parasites were added to all tubes, and all groups were kept at 25 ±1 °C. At 24, 48, and 72 hours, the number of live parasites was counted by Trypan Blue using a neobar slide and light microscope (Hemocytometer method). Then Half-maximal inhibitory concentration (IC50) value for the above extract was calculated using SigmaPlot™ 13 software. All steps of the experiment were done in triplicate and the results were considered as average.
Results: The IC50 content of methanolic extract of dandelion roots after 24, 48, and 72 hours on L. major were calculated to be 1.04, 0.9, and 0.68 µg/ml. The highest growth inhibition (100%) was observed at a concentration of 1200 μg/ml after 72 hours of exposure. There was a significant difference between the IC50 extract and amphotericin B drug after 24, 48 and 72 hours (P<0.05).
Conclusion: Methanolic extract of dandelion root in different concentrations has an inhibitory effect on the growth of L. major and it is suggested that more and more complete studies be performed on the components of this plant and the lethal effect of the parasite in vivo.
Type of Study: Original Article |
Subject:
انگل شناسی
Received: 2021/09/18 | Accepted: 2021/11/22 | Published: 2021/12/1
Received: 2021/09/18 | Accepted: 2021/11/22 | Published: 2021/12/1
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