Volume 16, Issue 9 (December 2022)                   Qom Univ Med Sci J 2022, 16(9): 756-767 | Back to browse issues page

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Adib S. Morphology and Molecular Studies and Differentiation Ability of Ovarian Stem Cells in Newborn Syrian Rats. Qom Univ Med Sci J 2022; 16 (9) :756-767
URL: http://journal.muq.ac.ir/article-1-3558-en.html
Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Branch of Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Abstract:   (659 Views)
Background and Objectives: The ability of ovarian stem cells (OSCs) to differentiate into oocyte-like and theca cells may lead to major advances in infertility treatment. However, little is known about the function, growth and differentiation potentials of these cells. In this study, we aim to examine the characteristics of OSCs obtained from newborn rats and assess their ability to differentiate into osteocyte-like and adipocyte-like cells.
Methods: In this study, OSCs were isolated from newborn Syrian rats and were then cultured. After the third passage, using the PCR method, the expression of stem cell markers (Sox2, Pou5f, and Nanog) was examined. Their potential to differentiate into osteocyte-like and adipocyte-like cells was examined by Alizarin red and Oil red staining, respectively.
Results: The isolated OSCs were adherent and morphologically similar to fibroblast cells, and had the power to multiply. They expressed stem cells markers. In addition, they were able to differentiate into adipocyte-like and osteocyte-like cells 21 days after being placed in the differentiation medium. In the osteocyte-like cells, fat droplets were observed after oil red staining.
Conclusion: The OSCs include multipotent stem cells that are able to express markers related to stem cells and can differentiate into osteocyte-like and adipocyte-like cells.
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Type of Study: Original Article | Subject: بافت شناسی و جنین شناسی
Received: 2022/08/28 | Accepted: 2022/10/16 | Published: 2022/11/1

References
1. Adib S, Valojerdi MR. Molecular assessment, characterization, and differentiation of theca stem cells imply the presence of mesenchymal and pluripotent stem cells in sheep ovarian theca layer. Res Vet Sci. 2017; 114:378-87. [DOI:10.1016/j.rvsc.2017.06.021] [PMID] [DOI:10.1016/j.rvsc.2017.06.021]
2. Porras-Gómez TJ, Moreno-Mendoza N. Neo-oogenesis in mammals. Zygote. 2017; 25(4):404-22. [DOI:10.1017/S0967199417000363] [PMID] [DOI:10.1017/S0967199417000363]
3. Dalman A, Totonchi M, Valojerdi MR. Human ovarian theca-derived multipotent stem cells have thepotential to differentiate into oocyte-like cells in vitro. Cell J. 2019; 20(4):527-36. [doi:10.22074/cellj.2019.5651]
4. Schubert C. Theca cell source. Biol Reprod. 2015; 93(2):26. [DOI:10.1095/biolreprod.115.131383] [DOI:10.1095/biolreprod.115.131383]
5. Young JM, McNeilly AS. Theca: the forgotten cell of the ovarian follicle. Reproduction. 2010; 140(4):489-504. [DOI:10.1530/REP-10-0094] [PMID] [DOI:10.1530/REP-10-0094]
6. Ghaneialvar H, Soltani L, Rahmani HR, Lotfi AS, Soleimani M. Characterization and classification of mesenchymal stem cells in several species using surface markers for cell therapy purposes. Indian J Clin Biochem. 2018; 33(1):46-52. [DOI:10.1007/s12291-017-0641-x] [PMID] [PMCID] [DOI:10.1007/s12291-017-0641-x]
7. Lin CS, Xin ZC, Dai J, Lue TF. Commonly used mesenchymal stem cell markers and tracking labels: Limitations and challenges. Histol Histopathol. 2013; 28(9):1109-16. [doi:10.14670/hh-28.1109]
8. Ghasemzadeh-Hasankolaei M, Eslaminejad MB, Sedighi-Gilani M. Derivation of male germ cells from ram bone marrow mesenchymal stem cells by three different methods and evaluation of their fate after transplantation into the testis. In Vitro Cell Dev Biol Anim. 2016; 52(1):49-61.[DOI:10.1007/s11626-015-9945-4] [PMID] [DOI:10.1007/s11626-015-9945-4]
9. Adib S, Tiraihi T, Darvishi M, Taheri T, Kazemi H. Cholinergic differentiation of neural stem cells generated from cell aggregates-derived from Human Bone marrow stromal cells. Tissue Eng Regen Med. 2015; 12:43-52. [DOI:10.1007/s13770-014-0019-6] [DOI:10.1007/s13770-014-0019-6]
10. Wang KH, Kao AP, Chang CC, Lin TC, Kuo TC. Upregulation of Nanog and Sox-2 genes following ectopic expression of Oct-4 in amniotic fluid mesenchymal stem cells. Biotechnol Appl Biochem. 2015; 62(5):591-7. [DOI:10.1002/bab.1315] [PMID] [DOI:10.1002/bab.1315]
11. Swain N, Thakur M, Pathak J, Swain B. SOX2, OCT4 and NANOG: The core embryonic stem cell pluripotency regulators in oral carcinogenesis. J Oral Maxillofac Pathol. 2020; 24(2):368-73. [DOI:10.4103/jomfp.JOMFP_22_20] [PMID] [PMCID] [DOI:10.4103/jomfp.JOMFP_22_20]
12. Cheng L, Thomas A, Roth LM, Zheng W, Michael H, Karim FWA. OCT4: a novel biomarker for dysgerminoma of the ovary. Am J Surg Pathol. 2004; 28(10):1341-6. [DOI:10.1097/01.pas.0000135528.03942.1f] [PMID] [DOI:10.1097/01.pas.0000135528.03942.1f]
13. Lee YM, Kumar BM, Lee JH, Lee WJ, Kim TH, Lee SL, et al. Characterisation and differentiation of porcine ovarian theca-derived multipotent stem cells. Vet J. 2013; 197(3):761-8. [DOI:10.1016/j.tvjl.2013.04.011] [PMID] [DOI:10.1016/j.tvjl.2013.04.011]
14. Sun Q, Nakata H, Yamamoto M, Kasugai S, Kuroda S. Comparison of gingiva-derived and bone marrow mesenchymal stem cells for osteogenesis. J Cell Mol Med. 2019; 23(11):7592-601. [DOI:10.1111/jcmm.14632] [PMID] [PMCID] [DOI:10.1111/jcmm.14632]
15. Sanghani-Kerai A, Black C, Cheng SO, Collins L, Schneider N, Blunn G, et al. Clinical outcomes following intra-articular injection of autologous adipose-derived mesenchymal stem cells for the treatment of osteoarthritis in dogs characterized by weight-bearing asymmetry. Bone Joint Res. 2021; 10(10):650-8. [DOI:10.1302/2046-3758.1010.BJR-2020-0540.R1] [PMID] [PMCID] [DOI:10.1302/2046-3758.1010.BJR-2020-0540.R1]

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