Ethics code: IR.MAZUMS.REC.1400.539
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nemati F, Abdullahpour R, Soleymani Rad S, Poorali H, Ghahari F. Biological Effects of Combining Ethanol Extract of Mord Leaf With Olive Oil on the Expression of Selected Growth Factors in Wound Healing of Male BALB/c Mice. Qom Univ Med Sci J 2023; 17 : 2732.1
URL: http://journal.muq.ac.ir/article-1-3589-en.html
URL: http://journal.muq.ac.ir/article-1-3589-en.html
Farkhondeh Nemati * 
1, Rohullah Abdullahpour2 , Samaneh Soleymani Rad3 , Hajar Poorali3 , Fatemeh Ghahari3
1, Rohullah Abdullahpour2 , Samaneh Soleymani Rad3 , Hajar Poorali3 , Fatemeh Ghahari3
1- Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran. , farkhondehnemati@gmail.com
2- Department of Animal Sciences, Faculty of Agriculture, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
3- Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
2- Department of Animal Sciences, Faculty of Agriculture, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
3- Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
Abstract: (637 Views)
Background and Objectives: Antibiotic treatment is used to reduce the bioburden of wounds. Medicinal plants can be an alternative method for wound treatment. This study aims to investigate the effects of Myrtus communis (Mord) leaf extract combined with olive oil on the wound healing process and the expression of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor-1 (FGF-1) and insulin-like growth factor-1 (IGF-1) genes in BALB/c mice.
Methods: The Mord leaf extract was mixed with olive oil in a volume ratio of 1:1. Thirty-six BALB/c male mice were divided into three groups of 12, including control, saline, and experimental. Then a 1×1 cm incision was made in their skin. On days 5, 10, 15, four mice from each group were randomly sacrificed and their skin samples were collected from the wound site. The real-time PCR method was used to measure the expression of four genes in the wound site. Statistical analysis was done by the analysis of variance and Tukey’s post hoc test. P<0.05 was considered statistically significant.
Results: The Mord leaf extract combined with olive oil increased the expression of VEGF, EGF, FGF-1, and IGF-1 genes (P<0.05). The effect was higher on the 15th day of the experiment.
Conclusion: The increase in the expression of VEGF, EGF, FGF-1, and IGF-1 genes due to the treatment using the combination of Mord leaf extract and olive oil can accelerate the wound healing process.
Methods: The Mord leaf extract was mixed with olive oil in a volume ratio of 1:1. Thirty-six BALB/c male mice were divided into three groups of 12, including control, saline, and experimental. Then a 1×1 cm incision was made in their skin. On days 5, 10, 15, four mice from each group were randomly sacrificed and their skin samples were collected from the wound site. The real-time PCR method was used to measure the expression of four genes in the wound site. Statistical analysis was done by the analysis of variance and Tukey’s post hoc test. P<0.05 was considered statistically significant.
Results: The Mord leaf extract combined with olive oil increased the expression of VEGF, EGF, FGF-1, and IGF-1 genes (P<0.05). The effect was higher on the 15th day of the experiment.
Conclusion: The increase in the expression of VEGF, EGF, FGF-1, and IGF-1 genes due to the treatment using the combination of Mord leaf extract and olive oil can accelerate the wound healing process.
Article number: 2732.1
Type of Study: Original Article |
Subject:
ژنتیک
Received: 2022/10/10 | Accepted: 2023/02/19 | Published: 2023/03/30
Received: 2022/10/10 | Accepted: 2023/02/19 | Published: 2023/03/30
References
1. Kebriti K, Naderi MS, Tabaie SM, Hesami Tackllou S. [Herbal medicine efficiency in wound healing (Persian)]. J Lasers Med. 2020; 16(4):41-32. [Link]
2. Alvites R, Branquinho M, Sousa AC, Lopes B, Sousa P, Maurício AC. Mesenchymal stem/stromal cells and their paracrine activity-immunomodulation mechanisms and how to influence the therapeutic potential. Pharmaceutics. 2022; 14(2):381. [DOI:10.3390/pharmaceutics14020381] [PMID] [PMCID] [DOI:10.3390/pharmaceutics14020381]
3. Garoufalia Z, Papadopetraki A, Karatza E, Vardakostas D, Philippou A, Kouraklis G, et al. Insulin-like growth factor-I and wound healing, a potential answer to non-healing wounds: A systematic review of the literature and future perspectives. Biomed Rep. 2021; 15(2):66. [DOI:10.3892/br.2021.1442] [PMID] [PMCID] [DOI:10.3892/br.2021.1442]
4. Scala P, Rehak L, Giudice V, Ciaglia E, Puca AA, Selleri C, et al. Stem cell and macrophage roles in skeletal muscle regenerative medicine. Int J Mol Sci. 2021; 22(19):10867. [DOI:10.3390/ijms221910867] [PMID] [PMCID] [DOI:10.3390/ijms221910867]
5. Garoufalia Z, Papadopetraki A, Karatza E, Vardakostas D, Philippou A, Kouraklis G, et al. Insulin-like growth factor-I and wound healing, a potential answer to non-healing wounds: A systematic review of the literature and future perspectives. Biomed Rep. 2021; 15(2):66. [DOI:10.3892/br.2021.1442] [PMID] [PMCID] [DOI:10.3892/br.2021.1442]
6. Blanco-Fernandez B, Castaño O, Mateos-Timoneda MÁ, Engel E, Pérez-Amodio S. Nanotechnology approaches in chronic wound healing. Adv Wound Care. 2021; 10(5):234-56. [DOI:10.1089/wound.2019.1094] [PMID] [PMCID] [DOI:10.1089/wound.2019.1094]
7. Zhu Y, Wang Y, Jia Y, Xu J, Chai Y. Roxadustat promotes angiogenesis through HIF-1α/VEGF/VEGFR2 signaling and accelerates cutaneous wound healing in diabetic rats. Wound Repair Regen. 2019; 27(4):324-34. [DOI:10.1111/wrr.12708] [PMID] [DOI:10.1111/wrr.12708]
8. Haj Ebrahimi Z, Naderi MS, Tabaie SM. [Study of gene expression and nanotechnology in wound healing process (Persian)]. J Lasers Med. 2016; 13(2 & 3):50-63. [Link]
9. Rezaie A, Mohajeri D, Khamene B, Nazeri M, Shishehgar R, Zakhireh S. Effect of myrtus communis on healing of the experimental skin wounds on rats and its comparison with zinc oxide. Curr Res J Biol Sci. 2012; 4(2):176-85. [Link]
10. Goli M, Samare Mousavi S, Rahbarian R, Rajabiyan M. [The effect of crocin and safranal components of saffron on skin wound healing in streptozotocin-induced diabetic rat (Persian)]. Jundishapur Sci Med J. 2022; 21(1):42-53. [DOI:10.32598/JSMJ.21.1.2358] [DOI:10.32598/JSMJ.21.1.2358]
11. Massoud D, Fouda MMA, Sarhan M, Salama SG, Khalifa HS. Topical application of Aloe gel and/or olive oil combination promotes the wound healing properties of streptozotocin-induced diabetic rats. Environ Sci Pollut Res Int. 2022; 29(39):59727-35. [DOI:10.1007/s11356-022-20100-9] [PMID] [DOI:10.1007/s11356-022-20100-9]
12. Nemati F, Ataee R, Gorji F, Houseini ST, Lotfvarzi A, Bagheri Hashem Abad A. [The effect of combining mord (Myrtus communis) leaf extract and olive oil (Olea europaea) in comparison with silver sulfadiazine cream on skin wound healing in Balb/c mice (Persian)]. Sci Res Q J Biol Sci Iran. 2022; 16(4):15-24. [link]
13. Aleem M, Anis M. Therapeutic potential of Habb-ul-Aas (Myrtus communis Linn.) with unani perspective and modern pharmacology: A review. J Pharmacogn Phytochemistry. 2021; 10(1):910-23. [DOI:10.22271/phyto.2021.v10.i1m.13452] [DOI:10.22271/phyto.2021.v10.i1m.13452]
14. Gharaboghaz MNZ, Farahpour MR, Saghaie S. Topical co-administration of Teucrium polium hydroethanolic extract and Aloe vera gel triggered wound healing by accelerating cell proliferation in diabetic mouse model. Biomed Pharmacother. 2020; 127:110189. [DOI:10.1016/j.biopha.2020.110189] [PMID] [DOI:10.1016/j.biopha.2020.110189]
15. Ishii T, Warabi E, Mann GE. Mechanisms underlying unidirectional laminar shear stress-mediated Nrf2 activation in endothelial cells: Amplification of low shear stress signaling by primary cilia. Redox Biol. 2021; 46:102103. [DOI:10.1016/j.redox.2021.102103] [PMID] [PMCID] [DOI:10.1016/j.redox.2021.102103]
16. Vijayan A, Sabareeswaran A, Vinod Kumar GS. PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing. Sci Rep. 2019; 9(1):19165. [DOI:10.1038/s41598-019-55214-7] [PMID] [PMCID] [DOI:10.1038/s41598-019-55214-7]
17. Basu S, Goswami AG, David LE, Mudge E. Psychological stress on wound healing: A silent player in a complex background. Int J Low Extrem Wounds. 2022; 15347346221077571.[DOI:10.1177/15347346221077571] [PMID] [DOI:10.1177/15347346221077571]
18. Balaji S, LeSaint M, Bhattacharya SS, Moles C, Dhamija Y, Kidd M, et al. Adenoviral-mediated gene transfer of insulin-like growth factor 1 enhances wound healing and induces angiogenesis. J Surg Res. 2014; 190(1):367-77. [DOI:10.1016/j.jss.2014.02.051] [PMID] [PMCID] [DOI:10.1016/j.jss.2014.02.051]
19. Johnson T, Zhao L, Manuel G, Taylor H, Liu D. Approaches to therapeutic angiogenesis for ischemic heart disease. J Mol Med. 2019; 97(2):141-51. [DOI:10.1007/s00109-018-1729-3] [PMID] [PMCID] [DOI:10.1007/s00109-018-1729-3]
20. Zhang Y, Wang ZL, Deng ZP, Wang ZL, Song F, Zhu LL. Emerging delivery strategies of platelet-rich plasma with hydrogels for wound healing. Adv Polym Technol. 2022; 2022:1-11. [DOI:10.1155/2022/5446291] [DOI:10.1155/2022/5446291]
21. Zhu J, Zhang M, Gao Y, Qin X, Zhang T, Cui W, et al. Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway. Signal Transduct Target Ther. 2020; 5(1):120. [DOI:10.1038/s41392-020-0173-3] [PMID] [PMCID] [DOI:10.1038/s41392-020-0173-3]
22. Koike Y, Yozaki M, Utani A, Murota H. Fibroblast growth factor 2 accelerates the epithelial-mesenchymal transition in keratinocytes during wound healing process. Sci Rep. 2020; 10(1):18545. [DOI:10.1038/s41598-020-75584-7] [PMID] [PMCID] [DOI:10.1038/s41598-020-75584-7]
23. Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin wound healing process and new emerging technologies for skin wound care and regeneration. Pharmaceutics. 2020; 12(8):735. [DOI:10.3390/pharmaceutics12080735] [PMID] [PMCID] [DOI:10.3390/pharmaceutics12080735]
24. Bártolo I, Reis RL, Marques AP, Cerqueira MT. Keratinocyte growth factor-based strategies for wound re-epithelialization. Tissue Eng Part B Rev. 2022; 28(3):665-76. [DOI:10.1089/ten.teb.2021.0030] [PMID] [DOI:10.1089/ten.teb.2021.0030]
25. Blaber SI, Diaz J, Blaber M. Accelerated healing in NONcNZO10/LtJ type 2 diabetic mice by FGF-1. Wound Repair Regen. 2015; 23(4):538-49. [DOI:10.1111/wrr.12305] [PMID] [DOI:10.1111/wrr.12305]
26. Ronca R, Giacomini A, Rusnati M, Presta M. The potential of fibroblast growth factor/fibroblast growth factor receptor signaling as a therapeutic target in tumor angiogenesis. Expert Opin Ther Targets. 2015; 19(10):1361-77. [DOI:10.1517/14728222.2015.1062475] [PMID] [DOI:10.1517/14728222.2015.1062475]
27. Michalak M, Pierzak M, Kręcisz B, Suliga E. Bioactive compounds for skin health: A review. Nutrients. 2021; 13(1):203. [DOI:10.3390/nu13010203] [PMID] [PMCID] [DOI:10.3390/nu13010203]
28. Melguizo-Rodríguez L, de Luna-Bertos E, Ramos-Torrecillas J, Illescas-Montesa R, Costela-Ruiz VJ, García-Martínez O. Potential effects of phenolic compounds that can be found in olive oil on wound healing. Foods. 2021; 10(7):1642. [DOI:10.3390/foods10071642] [PMID] [PMCID] [DOI:10.3390/foods10071642]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Owned by Qom University of Medical Sciences
Published by Qom University of Medical Sciences Press
eISSN: 2008-1375
pISSN: 1735-7799
Email: journal[at]muq.ac.ir
j.mua.ac[at]gmail.com
