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Rahimi O, Ahmadi A, Najafi G, Shalizar A. The Effect of Vitamin C on in Vitro Fertilization Process in Polycystic Mouse Oocytes. Qom Univ Med Sci J 2023; 17 : 2811.1
URL: http://journal.muq.ac.ir/article-1-3660-en.html
The Effect of Vitamin C on in Vitro Fertilization Process in Polycystic Mouse Oocytes
Ozra Rahimi1 , Abbas Ahmadi * 2, Golamreza Najafi1 , Ali Shalizar1
1- Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia, Iran.
2- Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia, Iran. , a.ahmady@urmia.ac.ir
Abstract:   (571 Views)
Background and Objectives: In this research, the effect of vitamin C as an antioxidant was investigated on in vitro fertilization and fetal growth in oocytes obtained from small laboratory mice with experimentally-induced polycystic ovary syndrome (PCOS) using estradiol valerate.
Methods: A number of 8-week-old Swiss-type adult mice were randomly selected and categorized into control (fed only with water and food) and PCOS (using estradiol valerate by intraperitoneal injection) groups. After a 2-month treatment period, hCG and Folligan drugs were injected intraperitoneally to stimulate ovulation. The oocytes were dissected, washed, and cultured in an HTF medium containing BSA (4 mg/mL). Oocytes obtained from mice were divided into different groups using culture media containing different doses of vitamin C. Then, the quality of the developed embryos, the percentage of cleavage, the number of arrested embryos, and the percentage of blastocysts created during 120 hours in each group were evaluated using an inverse microscope.
Results: The results of examining the percentage of fertilization, blastocyst, and other stages of fetal development showed that the induction of polycystic ovary syndrome caused a significant reduction in the stages of development and growth of the fetus compared to the control group. It has also caused an increase in type I and type II embryos.
Conclusion: Overall, using antioxidant vitamin C with the proper dose can positively affect fertility and fetal growth in the culture medium of in vitro fertilization.
Article number: 2811.1
Keywords: Ovarian cysts, In vitro fertilization, Fetal development, Ascorbic acid
Full-Text [PDF 4590 kb]   (362 Downloads) |   |   Full-Text (HTML)  (235 Views)  
Type of Study: Original Article | Subject: بافت شناسی و جنین شناسی
Received: 2023/01/28 | Accepted: 2023/03/18 | Published: 2023/08/1
References
1. Tahmasebi F, Movahedin M, Mazaheri Z. [Poly cystic ovary model as an elevated oxidative stress factor (Persian)]. J Mazandaran Univ Med Sci. 2015; 25(127):82-91. [Link]
2. Goodarzi MO, Dumesic DA, Chazenbalk G, Azziz R. Polycystic ovary syndrome: Etiology, pathogenesis and diagnosis. Nat Rev Endocrinol. 2011; 7(4):219-31. [DOI:10.1038/nrendo.2010.217] [PMID] [DOI:10.1038/nrendo.2010.217]
3. Panti AA, Shehu CE, Saidu Y, Tunau KA, Nwobodo EI, Jimoh A, et al. Oxidative stress and outcome of antioxidant supplementation in patients with polycystic ovarian syndrome (PCOS). Int J Reprod Contracept Obstet Gynecol. 2018; 7(5):1667-72. [DOI:10.18203/2320-1770.ijrcog20181892] [DOI:10.18203/2320-1770.ijrcog20181892]
4. Guérin P, El Mouatassim S, Ménézo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update. 2001; 7(2):175-89. [DOI:10.1093/humupd/7.2.175] [PMID] [DOI:10.1093/humupd/7.2.175]
5. Nadri B, Zeinoaldini S, Kohram H. Ascorbic acid effects on in vitro maturation of mouse oocyte with or without cumulus cell. Afr J Biotechnol. 2009; 8(20):5627-31. [Link]
6. Griesinger G, Franke K, Kinast C, Kutzelnigg A, Riedinger S, Kulin S, et al. Ascorbic acid supplement during luteal phase in IVF. J Assist Reprod Genet. 2002; 19(4):164-8. [DOI:10.1023/A:1014837811353] [PMID] [PMCID] [DOI:10.1023/A:1014837811353]
7. Lu X, Wu Z, Wang M, Cheng W. Effects of vitamin C on the outcome of in vitro fertilization-embryo transfer in endometriosis: A randomized controlled study. J Int Med Res. 2018; 46(11):4624-33. [DOI:10.1177/0300060518786918] [PMID] [DOI:10.1177/0300060518786918]
8. Kere M, Siriboon C, Lo NW, Nguyen NT, Ju JC. Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. J Reprod Dev. 2013; 59(1):78-84. [DOI:10.1262/jrd.2012-114] [PMID] [DOI:10.1262/jrd.2012-114]
9. Öztürkler Y, Yıldız S, Güngör Ö, Pancarcı ŞM, Kaçar C, Ari UÇ. The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes. Kafkas Univ Vet Fak Derg. 2010; 16(5):757-63. [DOI:10.9775/kvfd.2009.1646] [DOI:10.9775/kvfd.2009.1646]
10. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005; 3:28. [DOI:10.1186/1477-7827-3-28] [PMID] [DOI:10.1186/1477-7827-3-28]
11. Gong J, Wu DB, Zhang LL, Li J, Zhao X, Zhang D. [Study on the oxidative stress in the ovaries of a rat model of polycystic ovary (Chinese)]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2015; 46(2):238-47. [PMID]
12. Murri M, Luque-Ramírez M, Insenser M, Ojeda-Ojeda M, Escobar-Morreale HF. Circulating markers of oxidative stress and polycystic ovary syndrome (PCOS): A systematic review and meta-analysis. Hum Reprod Update. 2013; 19(3):268-88. [DOI:10.1093/humupd/dms059] [PMID] [DOI:10.1093/humupd/dms059]
13. Whitaker BD, Knight JW. Mechanisms of oxidative stress in porcine oocytes and the role of anti-oxidants. Reprod Fertil Dev. 2008; 20(6):694-702. [DOI:10.1071/RD08037] [PMID] [DOI:10.1071/RD08037]
14. Wang X, Falcone T, Attaran M, Goldberg JM, Agarwal A, Sharma RK. Vitamin C and vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocyst development rate. Fertil Steril. 2002; 78(6):1272-7.[DOI:10.1016/S0015-0282(02)04236-X] [PMID] [DOI:10.1016/S0015-0282(02)04236-X]
15. El AS, Naby KM, Sosa GA, Abouel-Roos ME, Ahmed YF. Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos. Asian Pac J Reprod. 2017; 6(2):85-8. [DOI:10.12980/apjr.6.20170207] [DOI:10.12980/apjr.6.20170207]
16. Barzegari Firouzabadi F. [Effects of ascorbic acid and FSH on the maturation of mice's oocytes and follicles (Persian)]. J Shahid Sadoughi Univ Med Sci. 2011; 19(5):586-97. [Link]
17. Musumeci G, Castrogiovanni P, Trovato FM, Parenti R, Szychlinska MA, Imbesi R. Pregnancy, embryo-fetal development and nutrition: Physiology around fetal programming. J Histol Histopathol. 2015; 2(1):1-6. [DOI:10.7243/2055-091X-2-1] [DOI:10.7243/2055-091X-2-1]
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