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Taebpour M, Javadi H, Sardarabadi H, Darvishi M H. Fabrication and Characterization of Silibinin-loaded Liposomes for Skin Wounds Healing. Qom Univ Med Sci J 2023; 17 : 2832.1
URL: http://journal.muq.ac.ir/article-1-3676-en.html
URL: http://journal.muq.ac.ir/article-1-3676-en.html
1- Department of Medical Biotechnology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2- Nanobiotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3- Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
4- Nanobiotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran. ,darvishi@alumnus.tums.ac.ir
2- Nanobiotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran.
3- Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran.
4- Nanobiotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran. ,
Abstract: (516 Views)
Background and Objectives: Silibinin, an antioxidant, is used to prevent and treat skin inflammatory disorders. This study aimed to develop a liposomal silibinin formulation to increase its bioavailability and water solubility and control its kinetic release to improve the healing process of skin wounds.
Methods: liposomes carrying silibinin were prepared using the thin film method. The loading capacity and drug release kinetics were evaluated using the reading drug absorption in the suitable wavelength. The particle size and surface potential (ζ-potential) of nanosystems were determined using dynamic light scattering technique. Lipid nanoparticle morphology was determined using a scanning electron microscope. An MTT assay was then used to determine the cytotoxicity on human skin fibroblasts. The obtained data were analyzed using a 1-way analysis of variance in SPSS software, version 18.
Results: The particle size and ζ-potential of liposomal nanoparticles with silibinin optimized liposomal silibinin are 112±9.07 nm and -14.5±2.2 mV, respectively. The encapsulation of silibinin in liposome was 84%±5.3%, and the total drug release at 37°C during 24 h was 67.3%±4.98%. In addition, the results of cellular toxicity showed that silibinin alone or liposomes containing silibinin do not have cytotoxicity on healthy human fibroblasts. Further, encapsulated silibinin versus its free form promotes the growth of fibroblast healthy cells.
Conclusion: The results show that a nanoliposomal silibinin formulation can be used as a nanodrug containing an effective herbal agent for speeding up the healing of skin wounds.
Methods: liposomes carrying silibinin were prepared using the thin film method. The loading capacity and drug release kinetics were evaluated using the reading drug absorption in the suitable wavelength. The particle size and surface potential (ζ-potential) of nanosystems were determined using dynamic light scattering technique. Lipid nanoparticle morphology was determined using a scanning electron microscope. An MTT assay was then used to determine the cytotoxicity on human skin fibroblasts. The obtained data were analyzed using a 1-way analysis of variance in SPSS software, version 18.
Results: The particle size and ζ-potential of liposomal nanoparticles with silibinin optimized liposomal silibinin are 112±9.07 nm and -14.5±2.2 mV, respectively. The encapsulation of silibinin in liposome was 84%±5.3%, and the total drug release at 37°C during 24 h was 67.3%±4.98%. In addition, the results of cellular toxicity showed that silibinin alone or liposomes containing silibinin do not have cytotoxicity on healthy human fibroblasts. Further, encapsulated silibinin versus its free form promotes the growth of fibroblast healthy cells.
Conclusion: The results show that a nanoliposomal silibinin formulation can be used as a nanodrug containing an effective herbal agent for speeding up the healing of skin wounds.
Article number: 2832.1
Type of Study: Original Article |
Subject:
سلولی و مولکولی
Received: 2023/02/14 | Accepted: 2023/06/12 | Published: 2023/08/1
Received: 2023/02/14 | Accepted: 2023/06/12 | Published: 2023/08/1
References
1. Chua AW, Khoo YC, Tan BK, Tan KC, Foo CL, Chong SJ. Skin tissue engineering advances in severe burns: Review and therapeutic applications. Burns Trauma. 2016; 4:3. [DOI:10.1186/s41038-016-0027-y] [PMID] [DOI:10.1186/s41038-016-0027-y]
2. Sundaramurthi D, Krishnan UM, Sethuraman S. Electrospun nanofibers as scaffolds for skin tissue engineering. Polym Rev. 2014; 54(2):348-76. [DOI:10.1080/15583724.2014.881374] [DOI:10.1080/15583724.2014.881374]
3. Simões D, Miguel SP, Ribeiro MP, Coutinho P, Mendonça AG, Correia IJ. Recent advances on antimicrobial wound dressing: A review. Eur J Pharm Biopharm. 2018; 127:130-41. [DOI:10.1016/j.ejpb.2018.02.022] [PMID] [DOI:10.1016/j.ejpb.2018.02.022]
4. Paul W. Advances in wound healing materials. Akron: Smithers Information Limited; 2015. [Link]
5. Salinas SG. Smart dressings based on nanostructured fibers containing natural origin antimicrobial and anti-inflammatory compounds. Zaragoza: Universidad de Zaragoza; 2021. [Link]
6. El Hassanen YA, Badran H, Abd EL-Rahman A, Badawy NM. Potential effect of milk thistle (Silybum marianum) on liver disorders induced by carbon tetrachloride. J Home Econ. 2021; 31(1):83-92. [Link]
7. Federico A, Dallio M, Loguercio C. Silymarin/silybin and chronic liver disease: A marriage of many years. Molecules. 2017; 22(2):191. [DOI:10.3390/molecules22020191] [PMID] [PMCID] [DOI:10.3390/molecules22020191]
8. Ding Y, Zhang S, Sun Z, Tong Z, Ge Y, Zhou L, et al. Preclinical validation of silibinin/albumin nanoparticles as an applicable system against acute liver injury. Acta Biomater. 2022; 146:385-95. [DOI:10.1016/j.actbio.2022.04.021] [PMID] [DOI:10.1016/j.actbio.2022.04.021]
9. Gehrcke M, Martins CC, de Bastos Brum T, da Rosa LS, Luchese C, Wilhelm EA, et al. Novel pullulan/gellan gum bilayer film as a vehicle for silibinin-loaded nanocapsules in the topical treatment of atopic dermatitis. Pharmaceutics. 2022; 14(11):2352. [DOI:10.3390/pharmaceutics14112352] [PMID] [DOI:10.3390/pharmaceutics14112352]
10. Bahramizadeh M, Bahramizadeh M, Kiafar B, Jafarian AH, Nikpoor AR, Hatamipour M, et al. Development, characterization and evaluation of topical methotrexate-entrapped deformable liposome on imiquimod-induced psoriasis in a mouse model. Int J Pharm. 2019; 569:118623. [DOI:10.1016/j.ijpharm.2019.118623] [PMID] [DOI:10.1016/j.ijpharm.2019.118623]
11. Chen J, Yan GJ, Hu RR, Gu QW, Chen ML, Gu W, et al. Improved pharmacokinetics and reduced toxicity of brucine after encapsulation into stealth liposomes: Role of phosphatidylcholine. Int J Nanomedicine. 2012; 7:3567-77. [DOI:10.2147/IJN.S32860] [PMID] [DOI:10.2147/IJN.S32860]
12. Barzegar-Jalali M, Adibkia K, Valizadeh H, Shadbad MR, Nokhodchi A, Omidi Y, et al. Kinetic analysis of drug release from nanoparticles. J Pharm Pharm Sci. 2008; 11(1):167-77. [DOI:10.18433/J3D59T] [PMID] [DOI:10.18433/J3D59T]
13. Schiweck H, Bär A, Vogel R, Schwarz E, Kunz M, Dusautois C, et al. Sugar Alcohols, Ullmann's Encyclopedia of Industrial Chemistry. Hoboken: Wiley Online Library; 2005. [DOI:10.1002/14356007.a25_413.pub2] [DOI:10.1002/14356007.a25_413.pub2]
14. Najafi N, Arabi M, Jafarzadeh H. [The healing power of Aloe vera mucilage: Induction of insulin-like growth factor gene expression and regeneration tissue in mouse damaged skin (Persian)]. J Shahrekord Univ Med Sci. 2014; 16(2):1-9. [Link]
15. Hedayati M, Yazdanparast R, Jafari BB, Azizi F. [Dendrostellera lessertii extract effects on TNF-α release and its receptors down regulation on cultured human monocytes (Persian)]. Res Med. 2005; 29(4):337-42. [Link]
16. Briuglia ML, Rotella C, McFarlane A, Lamprou DA. Influence of cholesterol on liposome stability and on in vitro drug release. Drug Deliv Transl Res. 2015; 5(3):231-42. [DOI:10.1007/s13346-015-0220-8] [PMID] [DOI:10.1007/s13346-015-0220-8]
17. Moghddam SR, Ahad A, Aqil M, Imam SS, Sultana Y. Formulation and optimization of niosomes for topical diacerein delivery using 3-factor, 3-level Box-Behnken design for the management of psoriasis. Mater Sci Eng C Mater Biol Appl. 2016; 69:789-97. [DOI:10.1016/j.msec.2016.07.043] [PMID] [DOI:10.1016/j.msec.2016.07.043]
18. Song X, Zhao Y, Wu W, Bi Y, Cai Z, Chen Q, et al. PLGA nanoparticles simultaneously loaded with vincristine sulfate and verapamil hydrochloride: Systematic study of particle size and drug entrapment efficiency. Int J Pharm. 2008; 350(1-2):320-9. [DOI:10.1016/j.ijpharm.2007.08.034] [PMID] [DOI:10.1016/j.ijpharm.2007.08.034]
19. Ahmad U, Faiyazuddin M, Hussain MT, Ahmad S, M Alshammari T, Shakeel F. Silymarin: An insight to its formulation and analytical prospects. Acta physiologiae plantarum. 2015; 37(253):1-17. [DOI:10.1007/s11738-015-2008-3] [DOI:10.1007/s11738-015-2008-3]
20. Rigon C, Marchiori MCL, da Silva Jardim F, Pegoraro NS, Chaves PDS, Velho MC, et al. Hydrogel containing silibinin nanocapsules presents effective anti-inflammatory action in a model of irritant contact dermatitis in mice. Eur J Pharm Sci. 2019; 137:104969. [DOI:10.1016/j.ejps.2019.104969] [PMID] [DOI:10.1016/j.ejps.2019.104969]
21. Samanta R, Pattnaik AK, Pradhan KK, Mehta BK, Pattanayak SP, Banerjee S. Wound healing activity of silibinin in mice. Pharmacognosy Res. 2016; 8(4):298-302. [DOI:10.4103/0974-8490.188880] [PMID] [DOI:10.4103/0974-8490.188880]
22. Gehrcke M, de Bastos Brum T, da Rosa LS, Ilha BD, Soares FZM, Cruz L. Incorporation of nanocapsules into gellan gum films: A strategy to improve the stability and prolong the cutaneous release of silibinin. Mater Sci Eng C Mater Biol Appl. 2021; 119:111624. [DOI:10.1016/j.msec.2020.111624] [PMID] [DOI:10.1016/j.msec.2020.111624]
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