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Ebadi Manas G. Pathological and Hormonal Alterations of the Testis in Balb/C Mice Treated With Spirotetramat Insecticide. Qom Univ Med Sci J 2023; 17 : 1895.2
URL: http://journal.muq.ac.ir/article-1-3700-en.html
URL: http://journal.muq.ac.ir/article-1-3700-en.html
Department of Biology Education, Farhangian University, Tehran, Iran. , Ebadimanas@gmail.com
Abstract: (465 Views)
Background and Objectives: The side effects of insecticides are undeniable due to their excessive use by farmers. This study aims to investigate the impact of spirotetramat insecticide on pathological and hormonal alteration in BALB/c mice testes.
Methods: In this experimental study, 24 adult BALB/c male mice, with a body weight range of 25-30 g, were obtained from the Animal House of Uremia University and examined. Mice were randomly divided into three groups of eight: control, experimental group 1, and experimental group 2. The mice of experimental group 1 received 2.5 mg/kg, and experimental group 2 received 10 mg/kg of spirotetramat. The control group received the same amount of distilled water for 21 days through the mouth by gavage. After this period, the mice were anesthetized and evaluated. Data were analyzed using a 1-way analysis of variance in SPSS version 19 software.
Results: The results of the study showed that spirotetramat increased the thickness of the tunica albuginea, the diameter of the seminiferous tubules, the differentiation index of the testis tube, and the spermiogenesis index, but it decreased the thickness of the epithelium of the spermatogenic tubules, the replacement index of active spermatogonia, Leydig cells, and FSH, LH, and testosterone hormone levels. The thickness of interstitial tissue, Sertoli, and lymphocyte cells did not change significantly.
Conclusion: By inhibiting the activity of acetyl coenzyme A, spirotetramat insecticide disrupts energy production and prevents the synthesis of lipids in the cells, finally decreasing spermatogenesis in mice.
Methods: In this experimental study, 24 adult BALB/c male mice, with a body weight range of 25-30 g, were obtained from the Animal House of Uremia University and examined. Mice were randomly divided into three groups of eight: control, experimental group 1, and experimental group 2. The mice of experimental group 1 received 2.5 mg/kg, and experimental group 2 received 10 mg/kg of spirotetramat. The control group received the same amount of distilled water for 21 days through the mouth by gavage. After this period, the mice were anesthetized and evaluated. Data were analyzed using a 1-way analysis of variance in SPSS version 19 software.
Results: The results of the study showed that spirotetramat increased the thickness of the tunica albuginea, the diameter of the seminiferous tubules, the differentiation index of the testis tube, and the spermiogenesis index, but it decreased the thickness of the epithelium of the spermatogenic tubules, the replacement index of active spermatogonia, Leydig cells, and FSH, LH, and testosterone hormone levels. The thickness of interstitial tissue, Sertoli, and lymphocyte cells did not change significantly.
Conclusion: By inhibiting the activity of acetyl coenzyme A, spirotetramat insecticide disrupts energy production and prevents the synthesis of lipids in the cells, finally decreasing spermatogenesis in mice.
Article number: 1895.2
Type of Study: Original Article |
Subject:
بافت شناسی و جنین شناسی
Received: 2023/03/26 | Accepted: 2023/04/24 | Published: 2023/08/1
Received: 2023/03/26 | Accepted: 2023/04/24 | Published: 2023/08/1
References
1. Mnif W, Hassine AI, Bouaziz A, Bartegi A, Thomas O, Roig B. Effect of endocrine disruptor pesticides: A review. Int J Environ Res Public Health. 2011; 8(6):2265-303. [DOI:10.3390/ijerph8062265] [PMID] [PMCID] [DOI:10.3390/ijerph8062265]
2. Nasirian H. Recent cockroach bacterial contamination trend in the human dwelling environments: A systematic review and meta-analysis. Bangladesh J Med Sci. 2019; 18(3): 540-5. [DOI:10.3329/bjms.v18i3.41623] [DOI:10.3329/bjms.v18i3.41623]
3. Nauen R, Reckmann U, Thomzik J, Thielert W. Biological profile of spirotetramat (Movento®)-a new two-way systemic (ambimobile) insecticide against sucking pest species. Bayer CropSci J. 2008; 61(2):245-78. [Link]
4. Alston DG, Drost D. Onion Thrips: Thrips tabaci. Logan: Utah State University Extension and Utah Plant Pest Diagnostic Laboratory; 2008. [Link]
5. Kuk YI, Burgos NR, Talbert RE. Cross-and multiple resistance of diclofop-resistant Lolium spp. Weed Sci. 2000; 48(4):412-9. [DOI:10.1614/0043-1745(2000)048[0412:CAMRODRL]2.0.CO;2] [DOI:10.1614/0043-1745(2000)048[0412:CAMRODRL]2.0.CO;2]
6. Bell JW. Petition for a three-year extension of exclusive use data protection for spirotetramat: As provided for under FIFRA section 3. Raleigh: Research Triangle Park; 2012. [Link]
7. Bekhakheche M, Manseur A, Masna F, Habbachi S, Habbachi W, Bairi A, et al. Chronic contamination in rats by reduced risk pesticides: Cases of spirotetramat and citrullus colocynthis (cucurbitaceae) extracts. World J Environ Biosci. 2018; 6(4):1-6. [Link]
8. Falcón-Etchechury M, Silveira-Gramont M, Robles-Sánchez R, Canett-Romero R, Ramos-Enríquez R, López-Cervantes G, et al. Spirotetramat induce cambios histológicos y bioquímicos en rata Wistar. Rev Toxicol. 2013; 30(2):215-7. [Link]
9. Kaboli Kafshgiri S, Parivar K, Baharara J, Hayati Roodbari N, Kerachian MA. [Comparison the effect of movento, a chemical pesticide, with chitosan, a biologic pesticide, on female reproductive system in Balb/C mice (Persian)]. Nova Biol Reperta. 2017; 3(4):279-87. [DOI:10.21859/acadpub.nbr.3.4.279] [DOI:10.21859/acadpub.nbr.3.4.279]
10. Sverdrup LC, Bjørge C, Eklo OM, Grung M, Källqvist T, Klingen I, et al. Risk assessment of the insecticide Movento 100 SC with the active substance spirotetramat: Opinion of the panel on plant protection products of the norwegian scientific committee for food safety. Sandakerveien: VKM; 2013. [Link]
11. Fischer R, Weiss H. Spirotetramat (Movento®)-discovery, synthesis and physico-chemical properties. Bayer CropSci J. 2008; 61(2):127-40. [Link]
12. Malhat F, Bakery M, Abdallah O, Youssef M, Ghany WAE, Abdallah A, et al. Dissipation kinetics and exposure of spirotetramat and pymetrozine in open fields, a prelude to risk assessment of green bean consumption. Environ Sci Pollut Res Int. 2023; 30(20):57747-58. [DOI:10.1007/s11356-023-26100-7] [PMID] [DOI:10.1007/s11356-023-26100-7]
13. Iftikhar A, Hafeez F, Aziz MA, Hashim M, Naeem A, Yousaf HK, et al. Assessment of sublethal and transgenerational effects of spirotetramat, on population growth of cabbage aphid, Brevicoryne brassicae L. (Hemiptera: Aphididae). Front Physiol. 2022; 13:1014190. [DOI:10.3389/fphys.2022.1014190] [PMID] [DOI:10.3389/fphys.2022.1014190]
14. Armand T, Korn L, Pichon E, Souquet M, Barbet M, Martin JL, et al. Efficiency and persistence of movento® treatment against myzus persicae and the transmission of aphid-borne viruses. Plants. 2021; 10(12):2747. [DOI:10.3390/plants10122747] [PMID] [DOI:10.3390/plants10122747]
15. Etchechury FM, Silveira-Gramont MI, Robles-Sánchez RM, Canett-Romero R, Ramos-Enríquez R, López-Cervantes JG, et al. Spirotetramat induces histological and biochemical changes in Wistar rats. Rev Toxicol. 2015; 30(2):215-7. [Link]
16. Ebadi Manas G, Hasanzadeh S, Parivar K. The effects of pyridaben pesticide on the histomorphometric, hormonal alternations and reproductive functions of BALB/c mice. Iran J Basic Med Sci. 2013; 16(10):1055-64. [PMID] [PMCID]
17. Ngoula F, Watcho P, Dongmo MC, Kenfack A, Kamtchouing P, Tchoumboué J. Effects of pirimiphos-methyl (an organophosphate insecticide) on the fertility of adult male rats. Afr Health Sci. 2007; 7(1):3-9. [DOI:10.5555/afhs.2007.7.1.3] [PMID] [PMCID]
18. Farag AT, El-Aswad AF, Shaaban NA. Assessment of reproductive toxicity of orally administered technical dimethoate in male mice. Reprod Toxicol. 2007; 23(2):232-8. [DOI:10.1016/j.reprotox.2006.12.003] [PMID] [DOI:10.1016/j.reprotox.2006.12.003]
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