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Mirzaei A, Taheri D, Zareian Baghdadabad L, Aghamir Mohammadali S M K. Formation of Bilateral Kidney and Bladder Stones After Using Ethylene Glycol and Ammonium Chloride in Wistar Rats. Qom Univ Med Sci J 2024; 18 : 2917.1
URL: http://journal.muq.ac.ir/article-1-3763-en.html
URL: http://journal.muq.ac.ir/article-1-3763-en.html
Akram Mirzaei1 
, Diana Taheri1 , Leila Zareian Baghdadabad1 , Seyed Mohammad Kazem Aghamir Mohammadali * 2
, Diana Taheri1 , Leila Zareian Baghdadabad1 , Seyed Mohammad Kazem Aghamir Mohammadali * 2
1- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran,
2- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran, ,mkaghamir@tums.ac.ir
2- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran, ,
Abstract: (864 Views)
Background and Objectives: The formation of kidney stones and their treatment is one of the most important challenges for physicians and patients. Kidney stone disease is one of the most common pathological diseases of the urinary tract. This study aims to show the effects of ethylene glycol and ammonium chloride on the formation of kidney and bladder stones in rats.
Methods: Twenty 4-week-old young Wistar rats were equally and randomly divided into two groups of control and case (5 males and five females). Kidney and bladder stone formation in the case group was induced by 1% ammonium chloride (administrated for 3 days) and 0.75% ethylene glycol (administrated for 30 days) added to the drinking water. The control group received drinking water only. The animals were anesthetized by ketamine/xylazine injection, and after a CT scan and confirmation of kidney and bladder stone formation, their kidneys and bladder were removed and fixated. Tissue samples were stained with hematoxylin & eosin staining method for histological studies. The slides were examined under a light microscope to count calcium oxalate deposits.
Results: Ethylene glycol and ammonium chloride caused stone formation in rats’ left and right kidneys and bladder. A significant decrease in urine volume and an increase in oxalate crystals were seen in the case group compared to the control group (P<0.05).
Conclusion: Administration of ethylene glycol plus ammonium chloride orally is the best way for the formation of kidney and bladder stones. Different therapeutic interventions can be tested in rats by creating kidney and bladder stones.
Methods: Twenty 4-week-old young Wistar rats were equally and randomly divided into two groups of control and case (5 males and five females). Kidney and bladder stone formation in the case group was induced by 1% ammonium chloride (administrated for 3 days) and 0.75% ethylene glycol (administrated for 30 days) added to the drinking water. The control group received drinking water only. The animals were anesthetized by ketamine/xylazine injection, and after a CT scan and confirmation of kidney and bladder stone formation, their kidneys and bladder were removed and fixated. Tissue samples were stained with hematoxylin & eosin staining method for histological studies. The slides were examined under a light microscope to count calcium oxalate deposits.
Results: Ethylene glycol and ammonium chloride caused stone formation in rats’ left and right kidneys and bladder. A significant decrease in urine volume and an increase in oxalate crystals were seen in the case group compared to the control group (P<0.05).
Conclusion: Administration of ethylene glycol plus ammonium chloride orally is the best way for the formation of kidney and bladder stones. Different therapeutic interventions can be tested in rats by creating kidney and bladder stones.
Article number: 2917.1
Type of Study: Original Article |
Subject:
نفرولوژی
Received: 2023/06/22 | Accepted: 2023/12/24 | Published: 2024/04/29
Received: 2023/06/22 | Accepted: 2023/12/24 | Published: 2024/04/29
References
1. Tanagho EA, McAninch JW. Smith's general urology. New York: Lange Medical Books/McGraw-Hill; 2004. [Link]
2. Coe FL, Evan A, Worcester E. Kidney stone disease. J Clin Invest. 2005; 115(10):2598-608. [DOI:10.1172/JCI26662] [PMID] [PMCID] [DOI:10.1172/JCI26662]
3. Menon M, Parulkar BG, Drach GW. Urinary lithiasis: Etiology, diagnosis and medical management. In: Walsh PC, Retik AB, Vaughan Jr ED, Wein AJ, editors. Campbell's urology. Philadelphia: Saunders Co; 1998. [Link]
4. Chiras DD. Human biology. Sudbury: Jones & Bartlett Publishers; 2013. [Link]
5. Scales CD Jr, Smith AC, Hanley JM, Saigal CS; Urologic Diseases in America Project. Prevalence of kidney stones in the United States. Eur Urol. 2012; 62(1):160-5. [DOI:10.1016/j.eururo.2012.03.052] [PMID] [PMCID] [DOI:10.1016/j.eururo.2012.03.052]
6. Kramer G, Klingler HC, Steiner GE. Role of bacteria in the development of kidney stones. Curr Opin Urol. 2000 ; 10(1):35-8. [DOI:10.1097/00042307-200001000-00009] [PMID] [DOI:10.1097/00042307-200001000-00009]
7. Collins CE. A short course in medical terminology. Baltimore: Wolters Kluwer Health; 2013. [Link]
8. Moe OW. Kidney stones: Pathophysiology and medical management. Lancet. 2006; 367(9507):333-44. [DOI:10.1016/S0140-6736(06)68071-9] [PMID] [DOI:10.1016/S0140-6736(06)68071-9]
9. Mandel NS, Mandel GS. Urinary tract stone disease in the United States veteran population. II. Geographical analysis of variations in composition. J Urol. 1989; 142(6):1516-21. [DOI:10.1016/S0022-5347(17)39145-0] [PMID] [DOI:10.1016/S0022-5347(17)39145-0]
10. Verkoelen CF, Romijn JC, de Bruijn WC, Boevé ER, Cao LC, Schröder FH. Association of calcium oxalate monohydrate crystals with MDCK cells. Kidney Int. 1995; 48(1):129-38. [DOI:10.1038/ki.1995.276] [PMID] [DOI:10.1038/ki.1995.276]
11. Jarald EE, Kushwah P, Edwin S, Asghar S, Patni SA. Effect of Unex on ethylene glycol-induced urolithiasis in rats. Indian J Pharmacol. 2011; 43(4):466-8. [DOI:10.4103/0253-7613.83124] [PMID] [PMCID] [DOI:10.4103/0253-7613.83124]
12. Wang R, Younis EM, Veeraraghavan VP, Tian C. Antiurolithiatic effect of fucoxanthin on ethylene glycol-induced renal calculus in experimental rats. J King Saud Univ Sci. 2020; 32(3):1896-901. [DOI:10.1016/j.jksus.2020.01.027] [DOI:10.1016/j.jksus.2020.01.027]
13. Hadjzadeh MA, Rad AK, Rajaei Z, Tehranipour M, Monavar N. The preventive effect of N-butanol fraction of Nigella sativa on ethylene glycol-induced kidney calculi in rats. Pharmacogn Mag. 2011; 7(28):338-43. [DOI:10.4103/0973-1296.90416] [PMID] [PMCID] [DOI:10.4103/0973-1296.90416]
14. Hadjzadeh MA, Khoei A, Hadjzadeh Z, Parizady M. Ethanolic extract of nigella sativa L seeds on ethylene glycol-induced kidney calculi in rats. Urol J. 2007; 4(2):86-90. [PMID]
15. Ahmadi M, Rad AK, Rajaei Z, Hadjzadeh MA, Mohammadian N, Tabasi NS. Alcea rosea root extract as a preventive and curative agent in ethylene glycol-induced urolithiasis in rats. Indian J Pharmacol. 2012; 44(3):304-7. [DOI:10.4103/0253-7613.96298] [PMID] [PMCID] [DOI:10.4103/0253-7613.96298]
16. Saeidi J, Lotfi M, Bozorgi H, Mehrzad J. The antiurolithiasic and hepatocurative activities of aqueous extracts of Petroselinum sativum on ethylene glycol-induced kidney calculi in rats. Sci Res Essays. 2012; 7(15):1577-83. [DOI:10.5897/SRE11.2130] [DOI:10.5897/SRE11.2130]
17. Habibian M, Peeri M, Azarbayjani M, Hedayati M. [Protective effect of aerobic exercise against some of proinflammatory cytokines-induced chronic nitric oxide synthase inhibition in renal tissue rats (Persian)]. J Babol Univ Med Sci. 2013; 15(1):30-7. [DOI:10.18869/acadpub.jbums.15.1.30]
18. Sorkhi H, Hedaiati F, Bijani A. [Efficacy of potassium citrate solution in children with urolithiasis (Persian)]. J Babol Univ Med Sci. 2011; 13(6):73-9. [Link]
19. Gupta M, Bhayana S, Sikka SK. Role of urinary inhibitors and promoters in calcium oxalate crystallisation. Int J Res Pharm chem. 2011; 1(4):793-8. [Link]
20. Jameson J, Loscalzo J. Harrison's nephrology and acid-base disorders, 2e. New York: McGraw Hill Professional; 2013. [Link]
21. Thangarathinam N, Jayshree N, Metha AV, Ramanathan L. Effect of polyherbal formulation on ethylene glycol induced urolithiasis. Int J Pharm Sci. 2013; 5(3):994-7. [Link]
22. Zia H, Khatami F, Rahimi MR, Aghamir SMK. Combined direct visual and imaging guided percutaneous nephrolithotomy: A novel technique. Transl Res Urol. 2021; 3(1):4-9. [DOI:10.22034/tru.2020.257597.1051]
23. Jafari Shahdani MR, Fattahi B, Mohseni MG, Aghamir SMK. Comparison of mini-perc and retrograde intrarenal surgery in residual stone fragments with hounsfield unit after percutaneous nephrolithotomy. Trans Res Urol. 2021; 3(2):40-4. [Link]
24. Itoh Y, Yasui T, Okada A, Tozawa K, Hayashi Y, Kohri K. Preventive effects of green tea on renal stone formation and the role of oxidative stress in nephrolithiasis. J Urol. 2005; 173(1):271-5. [DOI:10.1097/01.ju.0000141311.51003.87] [PMID] [DOI:10.1097/01.ju.0000141311.51003.87]
25. Moriyama MT, Suga K, Miyazawa K, Tanaka T, Higashioka M, Noda K, et al. Inhibitions of urinary oxidative stress and renal calcium level by an extract of quercus salicina blume/quercus stenophylla makino in a rat calcium oxalate urolithiasis model. Int J Urol. 2009; 16(4):397-401. [DOI:10.1111/j.1442-2042.2009.02268.x] [PMID] [DOI:10.1111/j.1442-2042.2009.02268.x]
26. Aslan Z, Aksoy L. Anti-inflammatory effects of royal jelly on ethylene glycol induced renal inflammation in rats. Int Braz J Urol. 2015; 41(5):1008-13. [DOI:10.1590/S1677-5538.IBJU.2014.0470] [PMID] [PMCID] [DOI:10.1590/S1677-5538.IBJU.2014.0470]
27. Cao Y, Gao X, Yang Y, Ye Z, Wang E, Dong Z. Changing expression profiles of long non-coding RNAs, mRNAs and circular RNAs in ethylene glycol-induced kidney calculi rats. BMC Genomics. 2018; 19(1):660. [DOI:10.1186/s12864-018-5052-8] [PMID] [PMCID] [DOI:10.1186/s12864-018-5052-8]
28. Kandhare AD, Patil MV, Bodhankar SL. L-Arginine attenuates the ethylene glycol induced urolithiasis in ininephrectomized hypertensive rats: Role of KIM-1, NGAL, and NOs. Ren Fail. 2015; 37(4):709-21. [DOI:10.3109/0886022X.2015.1011967] [PMID] [DOI:10.3109/0886022X.2015.1011967]
29. Yasir F, Wahab AT, Choudhary MI. Protective effect of dietary polyphenol caffeic acid on ethylene glycol-induced kidney stones in rats. Urolithiasis. 2018; 46(2):157-66. [DOI:10.1007/s00240-017-0982-1] [PMID] [DOI:10.1007/s00240-017-0982-1]
30. Salama AA, El-Kassaby MI, Hassan A. Anti-urolithiatic activity of Solanum nigrum hydroalcoholic extract in ethylene glycol-induced urolithiasis in rats. Egypt Pharm J. 2019; 18(4):311-9. [DOI:10.4103/epj.epj_21_19] [DOI:10.4103/epj.epj_21_19]
31. Bardaoui M, Sakly R, Neffati F, Najjar MF, El Hani A. Effect of vitamin A supplemented diet on calcium oxalate renal stone formation in rats. Exp Toxicol Pathol. 2010; 62(5):573-6. [DOI:10.1016/j.etp.2009.08.005] [PMID] [DOI:10.1016/j.etp.2009.08.005]
32. Corley RA, Bartels MJ, Carney EW, Weitz KK, Soelberg JJ, Gies RA, et al. Development of a physiologically based pharmacokinetic model for ethylene glycol and its metabolite, glycolic Acid, in rats and humans. Toxicol Sci. 2005; 85(1):476-90. [DOI:10.1093/toxsci/kfi119] [PMID] [DOI:10.1093/toxsci/kfi119]
33. Corley RA, Wilson DM, Hard GC, Stebbins KE, Bartels MJ, Soelberg JJ, et al. Dosimetry considerations in the enhanced sensitivity of male Wistar rats to chronic ethylene glycol-induced nephrotoxicity. Toxicol Appl Pharmacol. 2008; 228(2):165-78. [DOI:10.1016/j.taap.2007.11.024] [PMID] [DOI:10.1016/j.taap.2007.11.024]
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