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Honari H, Aghaie S M, Hajinormohamadi A, Samadpour Shahrak M H. Investigating the Immunogenicity of C-CFTXB Antigen Obtained From the Jellyfish (Chironex fleckeri) in Syrian Rats. Qom Univ Med Sci J 2024; 18 : 1360.3
URL: http://journal.muq.ac.ir/article-1-3844-en.html
URL: http://journal.muq.ac.ir/article-1-3844-en.html
Hossein Honari * 
1, Seyed Mojtaba Aghaie2 , Ashkan Hajinormohamadi2 , Mohammad Hassan Samadpour Shahrak2
1, Seyed Mojtaba Aghaie2 , Ashkan Hajinormohamadi2 , Mohammad Hassan Samadpour Shahrak2
1- Department of of Biology, School of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran. , honari.hosein@gmail.com
2- Department of of Biology, School of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.
2- Department of of Biology, School of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran.
Abstract: (517 Views)
Background and Objectives: The most lethal jellyfish are the Chironex fleckeri. Their stings are serious threat with economic and health implications. Nanoparticles have the ability to load a wide range of small medicinal and biological molecules. The small size of nanoparticles, due to the increase in the contact surface with the epithelial surface, has a greater potential for non-specific absorption into the cells. This study aims to assess the expression level of C-CFTXB protein in E. coli loaded to poly lactic-co-glycolic acid (PLGA) polymer to investigate its immunogenicity in Syrian rats.
Methods: The expression of C-CFTXB gene was assessed by isopropyl β-D-1-thiogalactopyranoside induction and the recombinant protein was confirmed by the western blot technique. The antigen was loaded to PLGA polymer. The size and zeta potential of nanoparticles containing protein were measured. Protein along with adjuvant PLGA nanoparticles in PBS were injected into the animal groups twice and four times. The amount of antibody in their serum was measured by ELISA test. In the end, the rats were challenged with Rhopilema nomadica jellyfish venom. Duncan’s test was used for statistical analysis.
Results: The size and zeta potential of nanoparticles containing protein were 200 nm and -20.2 mV, respectively. Electron microscope images showed that the nanoparticles were spherical. The results of the challenge test showed that the immunized rats tolerated 50×LD50 of jellyfish venom after 60 days.
Conclusion: Considering the lack of cardiotoxicity and neurotoxicity of C-CfTXB recombinant protein in rats, this antigen can be considered as a candidate vaccine against jellyfish venom.
Methods: The expression of C-CFTXB gene was assessed by isopropyl β-D-1-thiogalactopyranoside induction and the recombinant protein was confirmed by the western blot technique. The antigen was loaded to PLGA polymer. The size and zeta potential of nanoparticles containing protein were measured. Protein along with adjuvant PLGA nanoparticles in PBS were injected into the animal groups twice and four times. The amount of antibody in their serum was measured by ELISA test. In the end, the rats were challenged with Rhopilema nomadica jellyfish venom. Duncan’s test was used for statistical analysis.
Results: The size and zeta potential of nanoparticles containing protein were 200 nm and -20.2 mV, respectively. Electron microscope images showed that the nanoparticles were spherical. The results of the challenge test showed that the immunized rats tolerated 50×LD50 of jellyfish venom after 60 days.
Conclusion: Considering the lack of cardiotoxicity and neurotoxicity of C-CfTXB recombinant protein in rats, this antigen can be considered as a candidate vaccine against jellyfish venom.
Article number: 1360.3
Type of Study: Original Article |
Subject:
ایمونولوژی
Received: 2023/10/28 | Accepted: 2023/12/5 | Published: 2024/04/29
Received: 2023/10/28 | Accepted: 2023/12/5 | Published: 2024/04/29
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