Volume 13, Issue 12 (February 2020)
Qom Univ Med Sci J 2020, 13(12): 65-74 |
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َAkbari M E, Jamshidian A, Rasekh M, Esmaeelzadeh Behabadi S, Hajinezhad M R. The Effect of Hydroalcoholic Extract of Tamarix dioica Flower on Thioacetamide - Induced Histomorphological Changes in Rats. Qom Univ Med Sci J 2020; 13 (12) :65-74
URL: http://journal.muq.ac.ir/article-1-2566-en.html
URL: http://journal.muq.ac.ir/article-1-2566-en.html
Mohammad Ebrahim َAkbari1 
, Abbas Jamshidian * 2, Mehdi Rasekh3 , Sedighe Esmaeelzadeh Behabadi4 , Mohammad Reza Hajinezhad1
, Abbas Jamshidian * 2, Mehdi Rasekh3 , Sedighe Esmaeelzadeh Behabadi4 , Mohammad Reza Hajinezhad1
1- Department of Basic Veterinary Sciences, School of Veterinary Medicine, University of Zabol
2- Department of Pathobiology, School of Veterinary Medicine, University of Zabol ,hajinejad@gmail.com
3- Department of Clinical Sciences, School of Veterinary Medicine, University of Zabol
4- Department of Biology, School of Sciences, University of Zabol
2- Department of Pathobiology, School of Veterinary Medicine, University of Zabol ,
3- Department of Clinical Sciences, School of Veterinary Medicine, University of Zabol
4- Department of Biology, School of Sciences, University of Zabol
Abstract: (4172 Views)
Background and Objectives: The present experimental study was aimed to investigate the effect of T. dioica flower hydroalcoholic extract on thioacetamide induced histopathological changes.
Methods: Thirty adult male Wistar rats, were randomly divided into three groups of control (normal saline); negative control (thioacetamide 50 mg/kg) and group that received thioacetamide and T. dioica flower extract (200 mg/kg). The third group received the T. dioica flowers extract two weeks before the injection of thioacetamide and two weeks after the injection of thioacetamide. The healthy control group and the negative control group received normal saline. At the end of the experiment, blood samples, were obtained from the heart to determine lever enzymes aspartate aminotransferase (AST), alanine aminotransaminase (ALT), blood urea nitrogen (BUN), and creatinine. After euthanasia, tissue samples, were examined using a light microscope after staining with haematoxylin-eosin. Data were analyzed by one-way ANOVA statistical test. Statistical significance was considered as p < 0.05.
Results: The levels of serum liver enzyme, were significantly increased after the administration of thioacetamide. The administration of T. dioica extract significantly decreased serum ALT and AST levels compared to the negative control group (p < 0.05). Moreover, treatment with T. dioica extract significantly reduced serum BUN and creatinine levels compared to the negative control group (p < 0.05). In the histomorphometric investigation of liver, the fatty change and necrosis was lower in the T. dioica – treated group compared to the negative control group (p < 0.05). Renal histopathological examination also revealed lower kidney damage compared to the group treated with thioacetamide.
Conclusion: hydro-alcoholic extract of T. dioica flowers can reduce thioacetamide induced liver and kidney injury.
Methods: Thirty adult male Wistar rats, were randomly divided into three groups of control (normal saline); negative control (thioacetamide 50 mg/kg) and group that received thioacetamide and T. dioica flower extract (200 mg/kg). The third group received the T. dioica flowers extract two weeks before the injection of thioacetamide and two weeks after the injection of thioacetamide. The healthy control group and the negative control group received normal saline. At the end of the experiment, blood samples, were obtained from the heart to determine lever enzymes aspartate aminotransferase (AST), alanine aminotransaminase (ALT), blood urea nitrogen (BUN), and creatinine. After euthanasia, tissue samples, were examined using a light microscope after staining with haematoxylin-eosin. Data were analyzed by one-way ANOVA statistical test. Statistical significance was considered as p < 0.05.
Results: The levels of serum liver enzyme, were significantly increased after the administration of thioacetamide. The administration of T. dioica extract significantly decreased serum ALT and AST levels compared to the negative control group (p < 0.05). Moreover, treatment with T. dioica extract significantly reduced serum BUN and creatinine levels compared to the negative control group (p < 0.05). In the histomorphometric investigation of liver, the fatty change and necrosis was lower in the T. dioica – treated group compared to the negative control group (p < 0.05). Renal histopathological examination also revealed lower kidney damage compared to the group treated with thioacetamide.
Conclusion: hydro-alcoholic extract of T. dioica flowers can reduce thioacetamide induced liver and kidney injury.
Type of Study: Original Article |
Subject:
فیزیولوژی
Received: 2019/07/28 | Accepted: 2020/03/29 | Published: 2020/03/18
Received: 2019/07/28 | Accepted: 2020/03/29 | Published: 2020/03/18
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