Volume 14, Issue 3 (May 2020)                   Qom Univ Med Sci J 2020, 14(3): 45-53 | Back to browse issues page

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Ghorbani M, Khanbabaee R, Shriati M, Edalatmanesh M A. The Effect of Omega-3 on In Vitro Maturation of Mouse Oocytes at the Stage of Germinal Vesicle. Qom Univ Med Sci J 2020; 14 (3) :45-53
URL: http://journal.muq.ac.ir/article-1-2713-en.html
1- Department of Biology, Fars Science and Research Branch, Islamic Azad University
2- Department of Biology, Qaemshahr Branch, Islamic Azad University , khanbabaee@gmail.com
3- Department of Biology, Kazerun Branch, Islamic Azad University
4- Department of Biology, School of Sciences, Shiraz Branch, Islamic Azad University
Abstract:   (2580 Views)
Background and Objectives: Studies have shown that fatty acids affect maturation of oocytes, however, studies carried out on the effect of omega-3 on in vitro maturation of oocytes, are limited. The aim of this research was to investigate the effects of omega-3 on in vitro maturation of oocytes of mouse at the stage of germinal vesicle.
Methods: In this in vitro experimental study, NMRI mice aged 6 to 8 weeks, were super ovulated and killed 44 hours later, then their ovaries were removed, and immature oocytes were used for in vitro maturation. Immature oocytes were treated with omega-3 at doses of 10 and 100 µM, after 24 hours, the maturation of the oocytes was examined by invert microscope. The data were analyzed using chi square test. 
Results: Exposure of immature oocytes to 10 and 100 µM of omega-3 resulted in increased rate of mature oocytes compared to the control group (p < 0.05 and p < 0.01, respectively). Maturation rate was also higher in the group exposed to lower dose of omega-3 (10 µM) compared to the group exposed to higher dose (100 µM) of omega-3 (p < 0.01).
Conclusion: Addition of low doses of omega-3 to oocyte maturation medium, significantly increase the maturation rate of immature oocytes.
Full-Text [PDF 415 kb]   (732 Downloads)    
Type of Study: Original Article | Subject: فیزیولوژی
Received: 2020/01/13 | Accepted: 2020/06/6 | Published: 2020/06/30

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