Volume 14, Issue 3 (May 2020)                   Qom Univ Med Sci J 2020, 14(3): 45-53 | Back to browse issues page


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Ghorbani M, Khanbabaee R, Shriati M, Edalatmanesh M A. The Effect of Omega-3 on In Vitro Maturation of Mouse Oocytes at the Stage of Germinal Vesicle. Qom Univ Med Sci J 2020; 14 (3) :45-53
URL: http://journal.muq.ac.ir/article-1-2713-en.html
1- Department of Biology, Fars Science and Research Branch, Islamic Azad University
2- Department of Biology, Qaemshahr Branch, Islamic Azad University , khanbabaee@gmail.com
3- Department of Biology, Kazerun Branch, Islamic Azad University
4- Department of Biology, School of Sciences, Shiraz Branch, Islamic Azad University
Abstract:   (2580 Views)
Background and Objectives: Studies have shown that fatty acids affect maturation of oocytes, however, studies carried out on the effect of omega-3 on in vitro maturation of oocytes, are limited. The aim of this research was to investigate the effects of omega-3 on in vitro maturation of oocytes of mouse at the stage of germinal vesicle.
 
Methods: In this in vitro experimental study, NMRI mice aged 6 to 8 weeks, were super ovulated and killed 44 hours later, then their ovaries were removed, and immature oocytes were used for in vitro maturation. Immature oocytes were treated with omega-3 at doses of 10 and 100 µM, after 24 hours, the maturation of the oocytes was examined by invert microscope. The data were analyzed using chi square test. 
 
Results: Exposure of immature oocytes to 10 and 100 µM of omega-3 resulted in increased rate of mature oocytes compared to the control group (p < 0.05 and p < 0.01, respectively). Maturation rate was also higher in the group exposed to lower dose of omega-3 (10 µM) compared to the group exposed to higher dose (100 µM) of omega-3 (p < 0.01).
Conclusion: Addition of low doses of omega-3 to oocyte maturation medium, significantly increase the maturation rate of immature oocytes.
 
Full-Text [PDF 415 kb]   (732 Downloads)    
Type of Study: Original Article | Subject: فیزیولوژی
Received: 2020/01/13 | Accepted: 2020/06/6 | Published: 2020/06/30

References
1. 1. Jeromson S, Gallagher IJ, Galloway SD, Hamilton DL. Omega-3 fatty acids and skeletal muscle health. Mar Drugs 2015;13(11):6977-7004. PMID: 26610527 [DOI:10.3390/md13116977]
2. Cao Y, Lu L, Liang J, Liu M, Li X, Sun R, et al. Omega-3 fatty acids and primary and secondary prevention of cardiovascular disease. Cell Biochem Biophysics 2015;72(1):77-81. PMID: 25427890 [DOI:10.1007/s12013-014-0407-5]
3. Hoyos-Marulanda V, Alves BS, Rosa PR, Vieira AD, Gasperin BG, Mondadori RG, et al. Effects of polyunsaturated fatty acids on the development of pig oocytes in vitro following parthenogenetic activation and on the lipid content of oocytes and embryos. Anim Reprod Sci 2019;205:150-5. PMID: 31076217 [DOI:10.1016/j.anireprosci.2019.05.003]
4. Oseikria M, Elis S, Maillard V, Corbin E, Uzbekova S. N-3 polyunsaturated fatty acid DHA during IVM affected oocyte developmental competence in cattle. Theriogenology 2016;85(9):1625-34. PMID: 26898414 [DOI:10.1016/j.theriogenology.2016.01.019]
5. Davies MJ, Moore VM, Willson KJ, Van Essen P, Priest K, Scott H, et al. Reproductive Technologies and the risk of birth defects. N Engl J Med 2012;366(19):1803-13. PMID: 22559061 [DOI:10.1056/NEJMoa1008095]
6. Chang PL, Zeitoun KM, Chan LK, Thornton MH 2nd, Sauer MV. GnRH antagonist in older IVF patients. Retrieval rates and clinical outcome. J Reprod Med 2002;47(4):253-8. PMID: 12012875
7. Hwu YM, Lee RK, Chen CP, Su JT, Chen YM, Lin SP. Development of hatching blastocysts from immature human oocytes following in-vitro maturation and fertilization using a co-culture system. Hum Reprod 2005;13(7):1916-21. PMID: 9740449 [DOI:10.1093/humrep/13.7.1916]
8. Gilchrist RB, Luciano AM, Richani D, Zeng HT, Wang X, Vos MD, et al. Oocyte maturation and quality: role of cyclic nucleotides. Reproduction 2016;152(5):R143-57. PMID: 27422885 [DOI:10.1530/REP-15-0606]
9. Hardy K, Wright CS, Franks S, Winston RM. In vitro maturation of oocytes. Br Med Bull 2000;56(3):588-602. PMID: 11255547 [DOI:10.1258/0007142001903391]
10. Wonnacott KE, Kwong WY, Hughes J, Salter AM, Lea RG, Garnsworthy PC, et al. Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos. Reproduction 2010;139(1):57-69. PMID: 19789173 [DOI:10.1530/REP-09-0219]
11. Nehra D, Le HD, Fallon EM, Carlson SJ, Woods D, White YA, et al. Prolonging the female reproductive lifespan and improving egg quality with dietary omega-3 fatty acids. Aging Cell 2012;11(6):1046-54. PMID: 22978268 [DOI:10.1111/acel.12006]
12. Marei WF, Wathes DC, Fouladi-Nashta AA. The effect of linolenic acid on bovine oocyte maturation and development. Biol Reprod 2009;81(6):1064-72. PMID: 19587335 [DOI:10.1095/biolreprod.109.076851]
13. Pirro V, Oliveri P, Ferreira CR, González-Serrano AF, Machaty Z, Cooks RG. Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion. Anal Chim Acta 2014;848:51-60. PMID: 25263116 [DOI:10.1016/j.aca.2014.08.001]
14. Norberg B, Kleppe L, Andersson E, Thorsen A, Rosenlund G, Hamre K. Effects of dietary arachidonic acid on the reproductive physiology of female Atlantic cod (Gadus morhua L). Gen Comp Endocrinol 2017;250:21-35. PMID: 28576420 [DOI:10.1016/j.ygcen.2017.05.020]
15. Meher AP, Joshi AA, Joshi SR. Preconceptional omega-3 fatty acid supplementation on a micronutrient-deficient diet improves the reproductive cycle in Wistar rats. Reprod Fertil Dev 2013;25(7):1085-94. PMID: 23137932 [DOI:10.1071/RD12210]
16. Wakefield SL, Lane M, Schulz SJ, Hebart ML, Thompson JG, Mitchell M. Maternal supply of omega-3 polyunsaturated fatty acids alter mechanisms involved in oocyte and early embryo development in the mouse. Am J Physiol Endocrinol Metab 2008;294(2):E425-34. PMID: 18073322 [DOI:10.1152/ajpendo.00409.2007]
17. Wonnacott KE, Kwong WY, Hughes J, Salter AM, Lea RG, Garnsworthy PC, et al. Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos. Reproduction 2010;139(1):57-69. PMID: 19789173 [DOI:10.1530/REP-09-0219]
18. Bilby TR, Block J, do Amaral BC, Sa Filho O, Silvestre FT, Hansen PJ, et al. Effects of dietary unsaturated fatty acids on oocyte quality and follicular development in lactating dairy cows in summer. J Dairy Sci 2006;89(10):3891-903. PMID: 16960065 [DOI:10.3168/jds.S0022-0302(06)72432-8]
19. Liang S, Zhao MH, Ock SA, Kim NH, Cui XS. Fluoride impairs oocyte maturation and subsequent embryonic development in mice. Environ Toxicol 2016;31(11):1486-95. PMID: 26011085 [DOI:10.1002/tox.22153]
20. Zhang ZP, Liang GJ, Zhang XF, Zhang GL, Chao HH, Li L, et al. Growth of mouse oocytes to maturity from premeiotic germ cells in vitro. PLoS One 2012;7(7):e41771. PMID: 22848595 [DOI:10.1371/journal.pone.0041771]
21. Robinson RS, Pushpakumara PG, Cheng Z, Peters AR, Abayasekara DR, Wathes DC. Effects of dietary polyunsaturated fatty acids on ovarian and uterine function in lactating dairy cows. Reproduction 2002;124(1):119-31. PMID: 12090925 [DOI:10.1530/reprod/124.1.119]
22. Gulliver CE, Friend MA, King BJ, Clayton EH. The role of omega-3 polyunsaturated fatty acids in reproduction of sheep and cattle. Anim Reprod Sci 2012;131(1-2):9-22. PMID: 22386690 [DOI:10.1016/j.anireprosci.2012.02.002]
23. McKeegan PJ, Sturmey RG. The role of fatty acids in oocyte and early embryo development. Reprod Fertil Dev 2011;24(1):59-67. PMID: 22394718 [DOI:10.1071/RD11907]
24. Mahla AS, Chaudhari RK, Verma AK, Singh AK, Singh SK, Singh G, et al. Effect of dietary supplementation of omega-3 polyunsaturated fatty acid (PUFA) rich fish oil on reproductive performance of the goat (Capra hircus). Theriogenology 2017;99:79-89. PMID: 28708503 [DOI:10.1016/j.theriogenology.2017.05.023]
25. Lolicato F, Brouwers JF, de Lest CH, Wubbolts R, Aardema H, Priore P, et al. The cumulus cell layer protects the bovine maturing oocyte against fatty acid-induced lipotoxicity. Biol Reprod 2015;92(1):16. PMID: 25297544 [DOI:10.1095/biolreprod.114.120634]
26. Sorbera LA, Asturiano JF, Carrillo M, Zanuy S. Effects of polyunsaturated fatty acids and prostaglandins on oocyte maturation in a marine teleost, the European sea bass (Dicentrarchus labrax). Biol Reprod 2001;64(1):382-9. PMID: 11133697 [DOI:10.1095/biolreprod64.1.382]
27. Jungheim ES, Macones GA, Odem RR, Patterson BW, Lanzendorf SE, Ratts VS, et al. Associations between free fatty acids, cumulus oocyte complex morphology and ovarian function during in vitro fertilization. Fertil Steril 2011;95(6):1970-4. PMID: 21353671 [DOI:10.1016/j.fertnstert.2011.01.154]
28. Zarezadeh R, Mehdizadeh A, Leroy JL, Nouri M, Fayezi S, Darabi M. Action mechanisms of n-3 polyunsaturated fatty acids on the oocyte maturation and developmental competence: Potential advantages and disadvantages. J Cell Physiol 2019;234(2):1016-29. PMID: 30073662 [DOI:10.1002/jcp.27101]
29. Aardema H, Vos PL, Lolicato F, Roelen BA, Knijn HM, Vaandrager AB, et al. Oleic acid prevents detrimental effects of saturated fatty acids on bovine oocyte developmental competence. Biol Reprod 2011;85(1):62-9. PMID: 21311036 [DOI:10.1095/biolreprod.110.088815]
30. Ponter AA, Guyader-Joly C, Nuttinck F, Grimard B, Humblot P. Oocyte and embryo production and quality after OPU-IVF in dairy heifers given diets varying in their n-6/n-3 fatty acid ratio. Theriogenology 2012;78(3):632-45. PMID: 22537996 [DOI:10.1016/j.theriogenology.2012.03.009]

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