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Qom Univ Med Sci J 2021, 14(11): 20-29 Back to browse issues page
In Vitro Evaluation of Cytotoxic Effects of Nickel Oxide Nanoparticles on Human Neuroblastoma Cell Line (SH-SY5Y)
Mozhdeh Hajimohammadjafar tehrania1 , Mahsa Ale-Ebrahim * 2, Mojtaba Falahati3 , Shahram Zarabiyan1
1- Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
2- Department of Nanotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. , mahsa.alebrahim@yahoo.com
3- Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Abstract:   (1322 Views)
Background and Objectives: Despite the many applications of nickel oxide nanoparticles (Nio NPs) in industry and biomedicine, limited studies have been performed in the case of the cytotoxicity of these nanoparticles onnervous system yet. In many neurobiological studies, the human neuroblastoma cells (SH-SY5Y) wich have the ability to become adult human neurons have been used. The aim of the present study was to evaluate the cytotoxicity effects of nickel oxide nanoparticles on human neuroblastoma cell line (SH-SY5Y) in vitro.
 
Methods: The effect of different doses of NiO NPs (0, 1, 10, 20, 50, 100 μg/ml) on SH-SY5Y cell line was determined by MTT, lactate dehydrogenase (LDH), and caspase-3 activity tests. Statistical analysis was performed by using SPSS software and student t-test.
 
Results: Based on the results of MTT test, the viability rate of SH-SY5Y cells were decreased dose dependently after 24 hours treatment with NiO NPs, compared with control group. While there was no significant increase in levels of LDH enzyme compared with control group. The activity of caspase-3 enzyme was also significantly increased in a dose dependent pattern of nanoparticles.
 
Conclusion: The results of the present study showed that the Nickel oxide nanoparticles do not cause membrane damage in SH-SY5Y cells. While these nanoparticles induce their toxic effects by damaging the mitochondrial membrane, increasing the caspase 3 activity, and activating the apoptotic pathway.
 
Keywords: Apoptosis, Caspase 3, Cytotoxicity, L-Lactate Dehydro-genase, Nanoparticles, Nickel monoxide
Full-Text [PDF 764 kb]   (500 Downloads)    
Type of Study: Original Article | Subject: سلولی و مولکولی
Received: 2020/04/23 | Accepted: 2021/02/14 | Published: 2021/01/29
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Hajimohammadjafar tehrania M, Ale-Ebrahim M, Falahati M, Zarabiyan S. In Vitro Evaluation of Cytotoxic Effects of Nickel Oxide Nanoparticles on Human Neuroblastoma Cell Line (SH-SY5Y). Qom Univ Med Sci J 2021; 14 (11) :20-29
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Volume 14, Issue 11 (January 2021) Back to browse issues page
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