Volume 14, Issue 6 (August 2020)                   Qom Univ Med Sci J 2020, 14(6): 9-17 | Back to browse issues page

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Rahmani S, Rezaei M. Protective Effect of N-acetyl-cysteine Against Rat Liver Mitochondrial Toxicity Induced by CuSO4. Qom Univ Med Sci J 2020; 14 (6) :9-17
URL: http://journal.muq.ac.ir/article-1-2845-en.html
Protective Effect of N-acetyl-cysteine Against Rat Liver Mitochondrial Toxicity Induced by CuSO4
Sohrab Rahmani1 , Mohsen Rezaei * 2
1- MSc, Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2- Associate Professor, Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , rezaei.m@modares.ac.ir
Abstract:   (2204 Views)
Background and Objectives: Copper sulfate (CuSO4) is a toxic substance with a broad range of applications; however, the ingestion of high levels of it cause a wide range of complications in the liver tissue. According to previous studies, mitochondria are potential targets for CuSO4 toxicity. The present study aimed to examine the role of N-acetyl-cysteine as an antioxidant agent in the protection of mitochondria against toxicity induced by CuSO4.
 
Methods: This study was performed on isolated liver mitochondria extracted from male Wistar rats (180-200 g) by multiple centrifuges and finally divided into 5 different groups. Group 1 was the control group, while group 2 received a single dose of 106 µM of CuSo4. The other groups were pretreated with different concentrations of N-acetyl-cysteine (1, 2, 5 mM) and exposed to 106 µM of CuSO4. Subsequently, MTT assay, lipid peroxidation, and GSH (reduced glutathione) were determined in each group. Finally, the collected data were analyzed using the one-way analysis of variance test.
 
Results: The results of the present study revealed that significant changes in mitochondrial indexes such as mitochondrial complex Ⅱ function, mitochondrial glutathione reduction, and lipid peroxidation levels were evident in rat liver mitochondria exposed to CuSO4. On the other hand, it was found that pre-treatment with N-acetylcysteine efficiently inhibited CuSO4 toxicity.
  
Conclusion: The results confirmed the protective effects of N-acetyl-cysteine against CuSO4 toxicity on rat liver mitochondria, which may be due to its ability to scavenge reactive oxygen species.
Keywords: CuSO4, Isolated mitochondria, N-acetyl-cysteine, Reduced glutathione
Full-Text [PDF 631 kb]   (753 Downloads)    
Type of Study: Original Article | Subject: pharmacology
Received: 2020/06/18 | Accepted: 2020/08/26 | Published: 2020/08/31
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