Volume 14, Issue 8 (October 2020)
Qom Univ Med Sci J 2020, 14(8): 10-20 |
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Pirtaj Hamedani A, Behfar M, Tehrani A. Effect of Oral Administration of Silymarin on Healing Full-thickness Gastric Wound in Rats. Qom Univ Med Sci J 2020; 14 (8) :10-20
URL: http://journal.muq.ac.ir/article-1-2884-en.html
URL: http://journal.muq.ac.ir/article-1-2884-en.html
1- Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University
2- Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University ,m.behfar@urmia.ac.ir
3- Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University
2- Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University ,
3- Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University
Abstract: (2745 Views)
Background and Objectives: Gastrotomy can be accompanied by serious postoperative complications. In this regard, the present study aimed to evaluate the effects of oral administration of silymarin on the histopathological and biomechanical characteristics of the gastric wall after experimental gastrotomy in rats.
Methods: Full-thickness incision on the gastric wall and then suturing were conducted on 36 male Wistar rats. In the negative control group, 2 ml of normal saline was orally administered once a day (for 5 days). Moreover, omeprazole (3.6 mg/kg) and silymarin (50 mg/kg) in the positive control and treatment groups were administered in a similar manner, respectively. Euthanasia and sampling were performed on the 6th day. The data were analyzed using one-way analysis of variance and Kruskal-Wallis test.
Results: Histopathological findings demonstrated a significant increase in the maturation of fibroblasts, deposition and arrangement of collagen, angiogenesis, and formation of epithelial layer in the silymarin treatment group, compared to the two control groups. According to the results, the infiltration of inflammatory cells significantly reduced in the omeprazole and silymarin treatment groups in comparison to that reported for the negative control group. The levels of maximum strength, yield strength, stress, and energy absorption were higher in the silymarin-treated group, compared to those reported for other groups.
Conclusion: The obtained results of the present study showed that the oral administration of silymarin improved the tissue indicators of wound healing and its biomechanical features in the experimental model of the full-thickness gastric wound in rats. Based on the results, it is recommended to administer silymarin following gastrotomy.
Methods: Full-thickness incision on the gastric wall and then suturing were conducted on 36 male Wistar rats. In the negative control group, 2 ml of normal saline was orally administered once a day (for 5 days). Moreover, omeprazole (3.6 mg/kg) and silymarin (50 mg/kg) in the positive control and treatment groups were administered in a similar manner, respectively. Euthanasia and sampling were performed on the 6th day. The data were analyzed using one-way analysis of variance and Kruskal-Wallis test.
Results: Histopathological findings demonstrated a significant increase in the maturation of fibroblasts, deposition and arrangement of collagen, angiogenesis, and formation of epithelial layer in the silymarin treatment group, compared to the two control groups. According to the results, the infiltration of inflammatory cells significantly reduced in the omeprazole and silymarin treatment groups in comparison to that reported for the negative control group. The levels of maximum strength, yield strength, stress, and energy absorption were higher in the silymarin-treated group, compared to those reported for other groups.
Conclusion: The obtained results of the present study showed that the oral administration of silymarin improved the tissue indicators of wound healing and its biomechanical features in the experimental model of the full-thickness gastric wound in rats. Based on the results, it is recommended to administer silymarin following gastrotomy.
Type of Study: Original Article |
Received: 2020/07/27 | Accepted: 2020/10/7 | Published: 2020/11/30
Received: 2020/07/27 | Accepted: 2020/10/7 | Published: 2020/11/30
References
1. 1. Pfau PR. Removal and management of esophageal foreign bodies. Techn Gastrointest Endosc 2014;16(1):32-9. Link [DOI:10.1016/j.tgie.2013.10.004]
2. Sorensen LT, Hemmingsen U, Kallehave F, Wille-Jorgensen P, Kjaergaard J, Moller LN, et al. Risk factors for tissue and wound complications in gastrointestinal surgery. Ann Surg 2005;241(4):654-8. PMID: 15798468
3. Prado Filho OR, Fontes CER, Seidel AC, Tomasi MDA, Tomasi HDA. Colonic anastomosis with fibrin glue in diabetic rats. Magazine Braz Coll Surg 2008;35(5):329-33. Link [DOI:10.1590/S0100-69912008000500010]
4. Carucci LR, Turner MA, Conklin RC, DeMaria EJ, Kellum JM, Sugerman HJ. Roux-en-Y gastric bypass surgery for morbid obesity: Evaluation of postoperative extraluminal leaks with upper gastrointestinal series. Radiology 2006;238(1):119-27. PMID: 16373763 [DOI:10.1148/radiol.2381041557]
5. Saber A, Gad MA, Ellabban GM. Perforated duodenal ulcer in high risk patients: is percutaneous drainage justified? N Am J Med Sci 2012;4(1):35-9. PMID: 22393546 [DOI:10.4103/1947-2714.92902]
6. Kong F, Singh RP. Disintegration of solid foods in human stomach. J Food Sci 2008;73(5):67-80. PMID: 18577009 [DOI:10.1111/j.1750-3841.2008.00766.x]
7. Brandstaeter S, Fuchs SL, Aydin RC, Cyron CJ. Mechanics of the stomach: a review of an emerging field of biomechanics. GAMM‐Mitteilungen 2019;42(3):e201900001. Link [DOI:10.1002/gamm.201900001]
8. Johnson DA, Oldfield EC 4th. Reported side effects and complications of long-term proton pump inhibitor use: dissecting the evidence. Clin Gastroenterol Hepatol 2013;11(5):458-64. PMID: 23247326 [DOI:10.1016/j.cgh.2012.11.031]
9. Sarzynski E, Puttarajappa C, Xie Y, Grover M, Laird-Fick H. Association between proton pump inhibitor use and anemia: a retrospective cohort study. Dig Dis Sci 2011;56(8):2243-53. PMID: 21318590 [DOI:10.1007/s10620-011-1589-y]
10. Khalili H, Huang ES, Jacobson BC, Camargo CA, Feskanich D, Chan AT. Use of proton pump inhibitors and risk of hip fracture in relation to dietary and lifestyle factors: a prospective cohort study. BMJ 2012;344:e372. PMID: 22294756 [DOI:10.1136/bmj.e372]
11. Surai PF. Silymarin as a natural antioxidant: an overview of the current evidence and perspectives. Antioxidants (Basel) 2015;4(1):204-47. PMID: 26785346 [DOI:10.3390/antiox4010204]
12. Akbari-Kordkheyli V, Abbaszadeh-Goudarzi K, Nejati-Laskokalayeh M, Zarpou S, Khonakdar-Tarsi A. The protective effects of silymarin on ischemia-reperfusion injuries: a mechanistic review. Iran J Basic Med Sci 2019;22(9):968-76. PMID: 31807239
13. Oryan A, Tabatabaei-Naeini A, Moshiri A, Mohammadalipour A, Tabandeh MR. Modulation of cutaneous wound healing by silymarin in rats. J Wound Care 2012;21(9):457-64. PMID: 22990400 [DOI:10.12968/jowc.2012.21.9.457]
14. Ashkani-Esfahania S, Emamia Y, Esmaeilzadeha E, Bagheria F, Namazi MR, Keshtkar M. Silymarin enhanced fibroblast proliferation and tissue regeneration in full thickness skin wounds in rat models; a stereological study. J Saudi Soc Dermatol Dermatol Surg 2013;17(1):7-12. Link [DOI:10.1016/j.jssdds.2012.11.001]
15. Alarcon de la Lastra C, Martin MJ, Marhuenda E. Gastric anti-ulcer activity of silymarin, a lipoxygenase inhibitor, in rats. J Pharm Pharmacol 1992;44(11):929-31. PMID: 1361540 [DOI:10.1111/j.2042-7158.1992.tb03239.x]
16. Shin JH, Lee CW, Oh SJ, Yun J, Lee K, Park SK, et al. Protective effect of silymarin against ethanol-induced gastritis in rats: role of sulfhydryls, nitric oxide and gastric sensory afferents. Food Chem Toxicol 2013;55:353-7. PMID: 23357565 [DOI:10.1016/j.fct.2013.01.019]
17. Ersoy E, Ozturk V, Yazgan A, Ozdogan M, Gundogdu H. Comparison of the two types of bioresorbable barriers to prevent intra-abdominal adhesions in rats. J Gastrointest Surg 2009;13(2):282-6. PMID: 18777122 [DOI:10.1007/s11605-008-0678-5]
18. Abramov Y, Golden B, Sullivan M, Botros SM, Miller JJ, Alshahrour A, et al. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen 2007;15(1):80-6. PMID: 17244323 [DOI:10.1111/j.1524-475X.2006.00188.x]
19. Demidova-Rice TN, Hamblin MR, Herman IM. Acute and impaired wound healing: pathophysiology and current methods for drug delivery, part 1: Normal and chronic wounds: biology, causes, and approaches to care. Adv Skin Wound Care 2012;25(7):304-14. PMID: 22713781 [DOI:10.1097/01.ASW.0000416006.55218.d0]
20. Wallace JL. How do NSAIDs cause ulcer disease? Baillieres Best Pract Res Clin Gastroenterol 2000;14(1):147-59. PMID: 10749095 [DOI:10.1053/bega.1999.0065]
21. Hernández-Díaz S, Rodríguez LA. Steroids and risk of upper gastrointestinal complications. Am J Epidemiol 2001;153(11):1089-93. PMID: 11390328 [DOI:10.1093/aje/153.11.1089]
22. Al Rasheed NM, Faddah LM, Mohamed AM. Potential impact of silymarin in combination with chlorogenic acid and/or melatonin in combating cardiomyopathy induced by carbon tetrachloride. Saudi J Biol Sci 2014;21(3):265-74. PMID: 24955012 [DOI:10.1016/j.sjbs.2013.09.006]
23. Sharifi R, Pasalar P, Kamalinejad M, Dehpour AR, Tavangar SM, Paknejad M et al. The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model. Pharm Biol 2013;51(3):298-303. PMID: 23137300 [DOI:10.3109/13880209.2012.721789]
24. Ghebremariam YT, Cooke JP, Gerhart W, Griego C, Brower JB, Doyle-Eisele M, et al. Pleiotropic effect of the proton pump inhibitor esomeprazole leading to suppression of lung inflammation and fibrosis. J Transl Med 2015;13:249. PMID: 26231702 [DOI:10.1186/s12967-015-0614-x]
25. Tanigawa T, Watanabe T, Higuchi K, Machida H, Okazaki H, Yamagami H, et al. Lansoprazole, a proton pump inhibitor, suppresses production of tumor necrosis factor-alpha and interleukin-1beta induced by lipopolysaccharide and helicobacter pylori bacterial components in human monocytic cells via inhibition of activation of nuclear factor-kappab and extracellular signal-regulated kinase. J Clin Biochem Nutr 2009;45(1):86-92. PMID: 19590712 [DOI:10.3164/jcbn.08-267]
26. Martins de Oliveira R, Antunes E Pedrazzoli J, Jr-Gambero A. The inhibitory effects of H+ K+ ATPase inhibitors on human neutrophils in vitro: restoration by a K+ ionophore. Inflamm Res 2007;56(3):105-11. PMID: 17406807 [DOI:10.1007/s00011-006-6127-6]
27. Jones MK, Wang H, Peskar BM. Inhibition of angiogenesis by nonsteroidal anti-inflammatory drugs: insight into mechanisms and implications for cancer growth and ulcer healing. Nat Med 1999;5(12):1418-23. PMID: 10581086 [DOI:10.1038/70995]
28. Kren V, Walterova D. Silybin and silymarin-- new effects and applications. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2005;149(1):29-41. PMID: 16170386 [DOI:10.5507/bp.2005.002]
29. Jiang C, Agarwal R, Lu J. Anti-angiogenic potential of a cancer chemopreventive flavonoid antioxidant, silymarin: Inhibition of key attributes of vascular endothelial cells and angiogenic cytokine secretion by cancer epithelial cells. Biochem Biophys Res Commun 2000;276(1):371-8. PMID: 11006131 [DOI:10.1006/bbrc.2000.3474]
30. Gadad PC, Matthews KH, Knott RM. Silymarin released from sterile wafers restores glucose impaired endothelial cell migration. Int J Pharm 2013;457(1):40-9. PMID: 24055598 [DOI:10.1016/j.ijpharm.2013.09.006]
31. Niv Y, Banić M. Gastric barrier function and toxic damage. Dig Dis 2014;32(3):235-42. PMID: 24732189 [DOI:10.1159/000357855]
32. Sharifi R, Rastegar H, Kamalinejad M, Dehpour AR, Tavangar SM, Paknejad M, et al. Effect of topical application of silymarin (Silybum marianum) on excision wound healing in albino rats. Acta Med Iran 2012;50(9):583-8. PMID: 23165806
33. Schulze-Delrieu K, Herman RJ, Shirazi SS, Brown BP. Contractions move contents by changing the configuration of the isolated cat stomach. Am J Physiol 1998;274(2):359-69. PMID: 9486190 [DOI:10.1152/ajpgi.1998.274.2.G359]
34. Behfar M, Sarrafzadeh-Rezaei F, Hobbenaghi R, Delirezh N, Dalir-Naghadeh B. Enhanced mechanical properties of rabbit flexor tendons in response to intratendinous injection of adipose derived stromal vascular fraction. Curr Stem Cell Res Ther 2012;7(3):173-8. PMID: 22329584 [DOI:10.2174/157488812799859874]
35. Huilgol SV, Jamadar MG. Gastroprotective role of bioflavonoid silymarin in animal model of acute cold-restraint stress induced gastric ulceration. Injury 2013;6(1):40-3. Link
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