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Qom Univ Med Sci J 2021, 14(11): 69-77 Back to browse issues page
Protective Effects of Gamma Oryzanol on Renal and Brain Damage Following Experimental Renal Ischemia-reperfusion Injury among Rats
Yasin Bagheri1 , Daryoush Mohajeri * 2, Davoud Kazemi3
1- PhD Student of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2- Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran. , Daryoushmohajeri@yahoo.com
3- Assistant Professor, Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Abstract:   (1147 Views)
Background and Objectives: Renal ischemia/reperfusion injury is one of the major causes of acute renal failure. This study aimed to investigate the effects of gamma oryzanol administrated by gavage and intraperitoneal methods on the antioxidant function and status of the kidneys and brain tissue after the induction of ischemia-reperfusion injury in the kidneys of rats.
Methods: The statistical population of this study consisted of 24 male rats divided into four 6-member groups, including the sham group without ischemia, control group with ischemia-reperfusion induction, ischemia-reperfusion group receiving treatment with 100 mg/body weight intraperitoneally, and ischemia-reperfusion group administered with a dose of 100 mg/body weight by gavage method. Gamma oryzanol was administered 1 h followed by the onset of left renal ischemia for 30 min. After 6 h of reperfusion, blood creatinine and urea and antioxidant function of the kidneys and brain tissue were measured.
Results: The results showed that the administration of gamma oryzanol by both methods of intraperitoneal injection and gavage caused a significant reduction in creatinine and blood urea levels, compared to the control group (P<0.05). Moreover, the amounts of glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase, and total oxidant capacity of kidney and brain tissue were significantly increased in the groups receiving treatment with gamma oryzanol; however, malondialdehyde level was significantly reduced in the same group, compared to the control group (P<0.05).
Conclusion: Based on the results, gamma oryzanol improved renal function and reduced the negative severity of ischemia-reperfusion injury in renal and brain tissues. It was also found that the gavage method performed better than the intraperitoneal injection method.
Keywords: Gamma Orizanol, Ischemia-reperfusion, Kidney, Brain, Rat
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Type of Study: Original Article | Subject: فیزیولوژی
Received: 2020/10/28 | Accepted: 2021/02/20 | Published: 2021/01/29
1. 1. Ahmadvand H, Mahdavifard S. Protective effect of thioctic acid on renal ischemia-reperfusion injury in rat. Int J Prev Med 2019;10:176. DOI: 10.4103/ijpvm.IJPVM_396_17 [DOI:10.4103/ijpvm.IJPVM_396_17]
2. Nezamoleslami S, Sheibani M, Dehpour AR, Mobasheran P, Shafaroodi H. Glatiramer acetate attenuates renal ischemia reperfusion injury in rat model. Exp Mol Pathol 2020;112:104329. DOI: 10.1016/j.yexmp.2019.104329 [DOI:10.1016/j.yexmp.2019.104329]
3. Shiva N, Sharma N, Kulkarni YA, Mulay SR, Gaikwad AB. Renal ischemia/reperfusion injury: an insight on in vitro and in vivo models. Life Sci 2020;256:117860. DOI: 10.1016/j.lfs.2020.117860 [DOI:10.1016/j.lfs.2020.117860]
4. Wu L, Li Q, Liu S, An X, Huang Z, Zhang B, et al. Protective effect of hyperoside against renal ischemia-reperfusion injury via modulating mitochondrial fission, oxidative stress, and apoptosis. Free Rad Res 2019;53(7):727-36. DOI: 10.1080/10715762.2019.1623883 [DOI:10.1080/10715762.2019.1623883]
5. Doi K, Rabb H. Impact of acute kidney injury on distant organ function: recent findings and potential therapeutic targets. Kidney Int 2016;89(3):555-64. DOI: 10.1016/j.kint.2015.11.019 [DOI:10.1016/j.kint.2015.11.019]
6. Singbartl K, Joannidis M. Short-term effects of acute kidney injury. Crit Care Clin 2015;31(4):751-62. DOI: 10.1016/j.ccc.2015.06.010 [DOI:10.1016/j.ccc.2015.06.010]
7. Buys-Gonçalves GF, Abreu LA, Gregorio BM, Sampaio FJ, Pereira-Sampaio MA, de Souza DB. Antioxidants as renoprotective agents for ischemia during partial nephrectomy. Biomed Res Int 2019;2019:8575398. DOI: 10.1155/2019/8575398 [DOI:10.1155/2019/8575398]
8. Zweier JL, Talukder MH. The role of oxidants and free radicals in reperfusion injury. Cardiovasc Res 2006;70(2):181-90. DOI: 10.1016/j.cardiores.2006.02.025 [DOI:10.1016/j.cardiores.2006.02.025]
9. Frei B, Higdon JV. Antioxidant activity of tea polyphenols in vivo: evidence from animal studies. J Nutr 2003;133(10):3275S-84S. DOI: 10.1093/jn/133.10.3275S [DOI:10.1093/jn/133.10.3275S]
10. Mohajeri D, Mousavi G, Mansouri MB. Histopathological study on the effects of turmeric (Curcuma longa linn.) powdwer on renal ischemia-reperfusion injuryin rats. J Vet Clin Pathol 2012;6(1):1493-503.
11. Szcześniak K, Ostaszewski P, Ciecierska A, Sadkowski T. Investigation of nutriactive phytochemical-gamma‐oryzanol in experimental animal models. J Anim Physiol Anim Nutr 2016;100(4):601-17. DOI: 10.1111/jpn.12428 [DOI:10.1111/jpn.12428]
12. Akter S, Sasaki H, Uddin KR, Ikeda Y, Miyakawa H, Shibata S. Anxiolytic effects of γ-oryzanol in chronically-stressed mice are related to monoamine levels in the brain. Life Sci 2019;216:119-28. DOI: 10.1016/j.lfs.2018.11.042 [DOI:10.1016/j.lfs.2018.11.042]
13. Ghatak SB, Panchal SS. Renoprotective effects of oryzanol in an animal model of experimentally induced diabetic nephropathy. Oriental Pharm Exp Med 2014;14(1):55-67. DOI: 10.1007/s13596-013-0119-1 [DOI:10.1007/s13596-013-0119-1]
14. Sancaktutar AA, Bodakci MN, Hatipoglu NK, Soylemez H, Basarılı K, Turkcu G. The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats. Urol Ann 2014;6(1):46-50. DOI: 10.4103/0974-7796.127029 [DOI:10.4103/0974-7796.127029]
15. Kirkby K, Baylis C, Agarwal A, Croker B, Archer L, Adin C. Intravenous bilirubin provides incomplete protection against renal ischemia-reperfusion injury in vivo. Am J Physiol Renal Physiol 2007;292(2):F888-94. DOI: 10.1152/ajprenal.00064.2006 [DOI:10.1152/ajprenal.00064.2006]
16. Akerboom TP, Sies H. Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol 1981;77:373-82. DOI: 10.1016/s0076-6879(81)77050-2 [DOI:10.1016/S0076-6879(81)77050-2]
17. Bolann B, Ulvik R. Improvement of a direct spectrophotometric assay for routine determination of superoxide dismutase activity. Clin Chem 1991;37(11):1993-9. Link [DOI:10.1093/clinchem/37.11.1993]
18. Dawn-Linsley M, Ekinci FJ, Ortiz D, Rogers E, Shea TB. Monitoring thiobarbituric acid-reactive substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system. J Neurosci Methods 2005;141(2):219-22. DOI: 10.1016/j.jneumeth.2004.06.010 [DOI:10.1016/j.jneumeth.2004.06.010]
19. Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 1991;196(2-3):143-51. DOI: 10.1016/0009-8981(91)90067-m [DOI:10.1016/0009-8981(91)90067-M]
20. Rotruck JT, Pope AL, Ganther HE, Swanson A, Hafeman DG, Hoekstra W. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973;179(4073):588-90. DOI: 10.1126/science.179.4073.588 [DOI:10.1126/science.179.4073.588]
21. Minutoli L, Puzzolo D, Rinaldi M, Irrera N, Marini H, Arcoraci V, et al. ROS-mediated NLRP3 inflammasome activation in brain, heart, kidney, and testis ischemia/reperfusion injury. Oxid Med Cell Longev 2016;2016:2183026. DOI: 10.1155/2016/2183026 [DOI:10.1155/2016/2183026]
22. Smith SF, Hosgood SA, Nicholson ML. Ischemia-reperfusion injury in renal transplantation: 3 key signaling pathways in tubular epithelial cells. Kidney Int 2019;95(1):50-6. DOI: 10.1016/j.kint.2018.10.009 [DOI:10.1016/j.kint.2018.10.009]
23. Aboutaleb N, Jamali H, Abolhasani M, Toroudi HP. Lavender oil (Lavandula angustifolia) attenuates renal ischemia/reperfusion injury in rats through suppression of inflammation, oxidative stress and apoptosis. Biomed Pharmacother 2019;110:9-19. DOI: 10.1016/j.biopha.2018.11.045 [DOI:10.1016/j.biopha.2018.11.045]
24. Korkmaz A, Kolankaya D. Protective effect of rutin on the ischemia/reperfusion induced damage in rat kidney. J Surg Res 2010;164(2):309-15. DOI: 10.1016/j.jss.2009.03.022 [DOI:10.1016/j.jss.2009.03.022]
25. Kozuka C, Yabiku K, Sunagawa S, Ueda R, Taira S-i, Ohshiro H, et al. Brown rice and its component, γ-oryzanol, attenuate the preference for high-fat diet by decreasing hypothalamic endoplasmic reticulum stress in mice. Diabetes 2012;61(12):3084-93. DOI: 10.2337/db11-1767 [DOI:10.2337/db11-1767]
26. Liu M, Reddy NM, Higbee EM, Potteti HR, Noel S, Racusen L, et al. The Nrf2 triterpenoid activator, CDDO-imidazolide, protects kidneys from ischemia-reperfusion injury in mice. Kidney Int 2014;85(1):134-41. DOI: 10.1038/ki.2013.357 [DOI:10.1038/ki.2013.357]
27. Vomund S, Schäfer A, Parnham MJ, Brüne B, Von Knethen A. Nrf2, the master regulator of anti-oxidative responses. Int J Mol Sci 2017;18(12):2772. DOI: 10.3390/ijms18122772 [DOI:10.3390/ijms18122772]
28. Ma Q. Role of nrf2 in oxidative stress and toxicity. Annu Rev Pharmacol Toxicol 2013;53:401-26. DOI: 10.1146/annurev-pharmtox-011112-140320 [DOI:10.1146/annurev-pharmtox-011112-140320]
29. Hagl S, Berressem D, Grewal R, Sus N, Frank J, Eckert GP. Rice bran extract improves mitochondrial dysfunction in brains of aged NMRI mice. Nutr Neurosci 2016;19(1):1-10. DOI: 10.1179/1476830515Y.0000000040 [DOI:10.1179/1476830515Y.0000000040]
30. Ismail M, Al-Naqeeb G, Mamat WAA, Ahmad Z. Gamma-oryzanol rich fraction regulates the expression of antioxidant and oxidative stress related genes in stressed rat's liver. Nutr Metab 2010;7(1):23. DOI: 10.1186/1743-7075-7-23 [DOI:10.1186/1743-7075-7-23]
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Bagheri Y, Mohajeri D, Kazemi D. Protective Effects of Gamma Oryzanol on Renal and Brain Damage Following Experimental Renal Ischemia-reperfusion Injury among Rats. Qom Univ Med Sci J 2021; 14 (11) :69-77
URL: http://journal.muq.ac.ir/article-1-2961-en.html

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Volume 14, Issue 11 (January 2021) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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