Volume 14, Issue 11 (January 2021)                   Qom Univ Med Sci J 2021, 14(11): 1-10 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Najafifard M, Heidarieh N, Haeri Rohani A, Eidi A. Effect of Coumarin on Memory Retention, Tissue Index, and GABAA Receptor Gene Expression in the Hippocampus of Gonadectomized Adult Male Rats. Qom Univ Med Sci J 2021; 14 (11) :1-10
URL: http://journal.muq.ac.ir/article-1-2963-en.html
1- Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University
2- Department of Biology, Faculty of Sciences, Qom Branch, Islamic Azad University
Abstract:   (2441 Views)
Background and Objectives: Learning is essential for understanding mental disorders, normal behavior, and forgetfulness. In this regard, the hippocampus plays an important role in the learning process. It has been reported that gamma-aminobutyric acid (GABA) receptors in the hippocampus are involved in learning and memory mechanisms and some diseases, such as epilepsy and Alzheimer's. This study aimed to investigate the effect of coumarin on retention, tissue index, and GABA type A receptor gene expression in the hippocampus of male gonadectomy rats.
 
Methods: The population of this study consisted of 40 Wistar rats, which were randomized into 5 groups (n=8 each). These groups included healthy without treatment, gonadectomized without treatment, gonadectomized receiving solvent or Dimethyl sulfoxide, and gonadectomized receiving coumarin at a dose of 3.5 mg/kg. The treatment was administered intraperitoneally once daily in 2 weeks. A shuttle box was used to test the memory retention of the rats. At the end of the research process, the rats were exterminated in accordance with research ethics. After removing the brains of rats, in each group, in four brains histology test was implemented with Niels staining, and in four other brains, the hippocampus was removed quickly. The hippocampi were placed inside the micro type and frozen with liquid nitrogen. Finally, a gene expression test was taken from the hippocampus using a real-time polymerase chain reaction.
 
Results: Based on the findings, in the memory retention test of initial latency to enter the dark room (step through latency), the gonadectomy group showed a reduction, compared to the healthy group.  Moreover, a significant decrease was observed in the number of healthy hippocampal pyramidal neurons; however, GABAA gene expression showed no significant difference. In the gonadectomy groups receiving treatments with different doses of coumarin, the amount of STL  (Step Through Latency) and number of healthy pyramidal neurons in the memory retention test showed a significant decrease, compared to the gonadectomy group receiving solvent; nonetheless, a significant increase was revealed in the GABAA-α2 gene expression.
 
Conclusion: It can be concluded that Gonadectomy caused memory impairment and coumarin affects memory impairment by increasing the GABAA-α2 gene expression and decreasing the number of healthy hippocampal neurons.
 
Full-Text [PDF 1634 kb]   (818 Downloads)    
Type of Study: Original Article | Subject: فیزیولوژی
Received: 2020/10/31 | Accepted: 2021/01/30 | Published: 2021/01/29

References
1. Byrne JH. Learning and memory: a comprehensive reference. Massachusetts: Academic Press; 2017. Link
2. Helm K, Haberman R, Dean S, Hoyt E, Melcher T, Lund P, et al. GABAB receptor antagonist SGS742 improves spatial memory and reduces protein binding to the cAMP response element (CRE) in the hippocampus. Neuropharmacology 2005;48(7):956-64. DOI: 10.1016/j.neuropharm.2005.01.019 [DOI:10.1016/j.neuropharm.2005.01.019]
3. Leite JF, Cascio M. Structure of ligand-gated ion channels: critical assessment of biochemical data supports novel topology. Mol Cell Neurosci 2001;17(5):777-92. DOI: 10.1006/mcne.2001.0984 [DOI:10.1006/mcne.2001.0984]
4. Nelson TS, Holstein SE, Baird JP, Pittman DW. Selective stimulation of central GABAAα2, 3, 5 receptors increases intake and motivation to consume sucrose solution in rats. Neuroscience 2019;409:111-9. DOI: 10.1016/j.neuroscience.2019.04.040 [DOI:10.1016/j.neuroscience.2019.04.040]
5. Pourrabi SR, Mohajjel Nayebi AR, Hossini SE. Role of the androgen receptors in memory impairment in mature male rats. Exper Anim Biol 2012;1(2):25-32. Link
6. Harlow SD, Gass M, Hall JE, Lobo R, Maki P, Rebar RW, et al. Executive summary of the stages of reproductive aging workshop +10: addressing the unfinished agenda of staging reproductive aging. J Clin Endocrinol Metab 2012;97(4):1159-68. DOI: 10.1210/jc.2011-3362 [DOI:10.1210/jc.2011-3362]
7. Kittler JT, Moss SJ. Modulation of GABAA receptor activity by phosphorylation and receptor trafficking: implications for the efficacy of synaptic inhibition. Curr Opin Neurobiol 2003;13(3):341-7. DOI: 10.1016/s0959-4388(03)00064-3 [DOI:10.1016/S0959-4388(03)00064-3]
8. Skalicka-Woźniak K, Orhan IE, Cordell GA, Nabavi SM, Budzyńska B. Implication of coumarins towards central nervous system disorders. Pharmacol Res 2016;103:188-203. DOI: 10.1016/j.phrs.2015.11.023 [DOI:10.1016/j.phrs.2015.11.023]
9. Muke SA, Peshattivar VV, Kaikini AA, Bagle SR, Dighe V, Sathaye S. Neuroprotective effect of coumarin nasal formulation: kindling model assessment of epilepsy. Front Pharmacol 2018;9:992. DOI: 10.3389/fphar.2018.00992 [DOI:10.3389/fphar.2018.00992]
10. Jahani R, Khaledyan D, Jahani A, Jamshidi E, Kamalinejad M, Khoramjouy M, et al. Evaluation and comparison of the antidepressant-like activity of Artemisia dracunculus and Stachys lavandulifolia ethanolic extracts: an in vivo study. Res Pharm Sci 2019;14:(6) 544-53. DOI: 10.4103/1735-5362.272563 [DOI:10.4103/1735-5362.272563]
11. Alaghemandan Motlagh N, Rouhani AH, Zarindast MR, Nasehi M. Analysis and identification of fungal skin infection Caspian salmon Salmo trutta caspius on farms Mazandaran province aquaculture. J Anim Biol 2014;6(4):51-9. Link
12. Cheriyan BV Sr, Kadhirvelu P Sr, Nadipelly J Jr, Shanmugasundaram J, Sayeli V Sr, Subramanian V Sr. Anti-nociceptive effect of 7-methoxy coumarin from Eupatorium Triplinerve vahl (Asteraceae). Pharmacogn Mag 2017;13(49):81-4. DOI: 10.4103/0973-1296.197650
13. Ebrahimzadeh M, Shahabi P, Mohaddes G, Babri S, Mohammadi M, Moslem A, et al. Effect of testosterone on memory and BDNF levels of hippocampus in gonadectomized diabetic rats. Biosci Biotechnol Res Asia 2015;12(3):2433-40. Link [DOI:10.13005/bbra/1921]
14. Alipour M, Adineh F, Mosatafavi H, Aminabadi A, Monirinasab H, Jafari MR. Effect of chronic intraperitoneal aminoguanidine on memory and expression of Bcl-2 family genes in diabetic rats. Can J Physiol Pharmacol 2015;94(6):669-75. DOI: 10.1139/cjpp-2015-0357 [DOI:10.1139/cjpp-2015-0357]
15. Kandel ER, Schwartz JH, Jessell TM, Siegelbaum S, Hudspeth AJ, Mack S. Principles of neural science. New York: McGraw-Hill; 2000. Link
16. Annovazzi L, Mellai M, Caldera V, Valente G, Schiffer D. SOX2 expression and amplification in gliomas and glioma cell lines. Cancer Genomics Proteomics 2011;8(3):139-47. Link
17. Morris RG, Moser EI, Riedel G, Martin SJ, Sandin J, Day M, et al. Elements of a neurobiological theory of the hippocampus: the role of activity-dependent synaptic plasticity in memory. Philos Trans R Soc Lond B Biol Sci 2003;358(1432):773-86. DOI: 10.1098/rstb.2002.1264 [DOI:10.1098/rstb.2002.1264]
18. Ghahramani P, Harooni HE, Fatemi Tabatabaei SR, Moazedi AA. Effects of zinc chloride on passive avoidance memory of male rats, in gonadectomized peri-pubertaly. Stud Med Sci 2018;28(11):708-14. Link
19. Whittle S, Yap MB, Sheeber L, Dudgeon P, Yücel M, Pantelis C, et al. Hippocampal volume and sensitivity to maternal aggressive behavior: a prospective study of adolescent depressive symptoms. Dev Psychopathol 2011;23(1):115-29. DOI: 10.1017/S0954579410000684 [DOI:10.1017/S0954579410000684]
20. Sperk G, Schwarzer C, Tsunashima K, Fuchs K, Sieghart W. GABA(A) receptor subunits in the rat hippocampus I: immunocytochemical distribution of 13 subunits. Neuroscience 1997;80(4):987-1000. DOI: 10.1016/s0306-4522(97)00146-2 [DOI:10.1016/S0306-4522(97)00146-2]
21. Myers JF, Comley RA, Gunn RN. Quantification of [11C] Ro15-4513 GABAAα5 specific binding and regional selectivity in humans. J Cereb Blood Flow Metab 2017;37(6):2137-8. DOI: 10.1177/0271678X16661339 [DOI:10.1177/0271678X16661339]
22. Moghadami S, Jahanshahi M, Sepehri H, Amini H. Gonadectomy reduces the density of androgen receptor-immunoreactive neurons in male rat's hippocampus: testosterone replacement compensates it. Behav Brain Funct 2016;12(1):5. DOI: 10.1186/s12993-016-0089-9 [DOI:10.1186/s12993-016-0089-9]
23. Deutsch SI, Mastropaolo J, Hitri A. GABA-active steroids: endogenous modulators of GABA-gated chloride ion conductance. Clin Neuropharmacol 1992;15(5):352-64. Link [DOI:10.1097/00002826-199210000-00002]
24. Narenji SA, Naghdi N, Azadmanesh K, Edalat R. 3α-diol administration decreases hippocampal PKA (II) mRNA expression and impairs Morris water maze performance in adult male rats. Behav Brain Res 2015;280:149-59. DOI: 10.1016/j.bbr.2014.11.038 [DOI:10.1016/j.bbr.2014.11.038]
25. Orchinik M, Weiland NG, McEwen BS. Adrenalectomy selectively regulates GABAA receptor subunit expression in the hippocampus. Mol Cell Neurosci 1994;5(5):451-8. DOI: 10.1006/mcne.1994.1055 [DOI:10.1006/mcne.1994.1055]
26. Łuszczki JJ, Andres-Mach M, Gleńsk M, Skalicka-Woźniak K. Anticonvulsant effects of four linear furanocoumarins, bergapten, imperatorin, oxypeucedanin, and xanthotoxin, in the mouse maximal electroshock-induced seizure model: a comparative study. Pharmacol Rep 2010;62(6):1231-6. DOI: 10.1016/s1734-1140(10)70387-x [DOI:10.1016/S1734-1140(10)70387-X]
27. Stolt AC, Schröder H, Neurath H, Grecksch G, Höllt V, Meyer MR, et al. Behavioral and neurochemical characterization of kratom (Mitragyna speciosa) extract. Psychopharmacology 2014;231(1):13-25. DOI: 10.1007/s00213-013-3201-y [DOI:10.1007/s00213-013-3201-y]
28. Hofmann JI, Schwarz C, Rudolph U, Antkowiak B. Effects of diazepam on low-frequency and high-frequency electrocortical γ-power mediated by α1-and α2-GABAA receptors. Intl J Mol Sci 2019;20(14):3486. DOI: 10.3390/ijms20143486 [DOI:10.3390/ijms20143486]
29. Nomura T, Hawkins NA, Kearney JA, George AL Jr, Contractor A. Potentiating α2 subunit containing perisomatic GABAA receptors protects against seizures in a mouse model of Dravet syndrome. J Physiol 2019;597(23):4293-307. DOI: 10.1113/JP277651 [DOI:10.1113/JP277651]
30. Kowalczyk P, Madej A, Paprocki D, Szymczak M, Ostaszewski R. Coumarin derivatives as new toxic compounds to selected K12, R1-R4 E. coli Strains. Materials 2020;13(11):2499. DOI: 10.3390/ma13112499 [DOI:10.3390/ma13112499]
31. Lončar M, Jakovljević M, Šubarić D, Pavlić M, Buzjak Služek V, Cindrić I, et al. Coumarins in food and methods of their determination. Foods 2020;9(5):645. DOI: 10.3390/foods9050645 [DOI:10.3390/foods9050645]

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Qom University of Medical Sciences Journal

Designed & Developed by : Yektaweb