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Qom Univ Med Sci J 2021, 14(12): 32-40 Back to browse issues page
Effect of N-acetylcysteine on Human Sperm Parameters and DNA Damage in Frozen-thawed Sperm Samples of Asthenozoospermic Men
Rahil Jannatifar1 , Hamid Piroozmanesh * 2, Zeinab Jannatifar3
1- PhD, Department of Reproductive Biology, the Academic Center for Education, Culture and Research, Qom branch, Iran.
2- جهاد دانشگاهی , HP457@YAHOO.COM
3- Student Research Committee, School of Dentistry, Qom University of Medical Sciences, Qom, Iran.
Abstract:   (999 Views)
Background and Objectives: The semen cryopreservation is not only required for improving the success chance of assisted reproductive technologies fertility but also is performed for men undergoing chemotherapy, radiotherapy, and surgery, and facing ejaculation problems. This study aimed to investigate the effects of N-acetyl-cysteine on human sperm parameters and DNA damage in frozen-thawed sperm samples of patients with asthenozoospermia.
Methods: In this study, 20 patients with asthenozoospermia referring to Jihad University Infertility Treatment Center, Qom, Iran, were evaluated in three groups, namely control, freezing, and freezing + N-acetyl-cysteine (1mg/ml). Sperm parameters, viability, and DNA damage were respectively assessed using the World Health Organization guideline (2010), eosin-nigrosin staining, and sperm chromatin dispersion kit, respectively in all three groups. The statistical analysis was performed using one-way ANOVA and Tukey post hoc test. The significance level was considered at the p-value of < 0.05.
Results: The freezing process resulted in a decrease in sperm motility parameters, and the addition of the antioxidant N-acetyl-cysteine improved sperm motility, morphology, and sperm viability. The addition of N-acetyl-cysteine could reduce DNA damage (P<0.05).
Conclusion: Our results showed that N-acetyl-cysteine ​​could reduce the disruptive effects of the freezing-thawing process.  
Keywords: DNA damage, N-acetyl-cysteine, Sperm.
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Type of Study: Original Article | Subject: علوم پایه
Received: 2020/11/29 | Accepted: 2021/04/6 | Published: 2021/02/28
1. Decherney AH. Principles & Practice of Assisted Reproductive Technology (3 Vols): JP Medical Ltd; 2013.
2. Esteves SC, Roque M, Bedoschi GM, Conforti A, Humaidan P, Alviggi CJFie. Defining low prognosis patients undergoing assisted reproductive technology: POSEIDON criteria-the why. 2018;9:461. [DOI:10.3389/fendo.2018.00461]
3. Direkvand Moghaddam A, Delpisheh A, Sayehmiri KJQUoMSJ. An investigation of the worldwide prevalence of infertility as a systematic review. 2016;10(1):76-87.
4. Ricci E, Viganò P, Cipriani S, Somigliana E, Chiaffarino F, Bulfoni A, et al. Coffee and caffeine intake and male infertility: a systematic review. 2017;16(1):37. [DOI:10.1186/s12937-017-0257-2]
5. Mocé E, Fajardo AJ, Graham JKJE. Human sperm cryopreservation. 2016;1(1):86-91.
6. Zandiyeh S, Shahverdi A, Ebrahimi B, Sabbaghian MJRB. A novel approach for human sperm cryopreservation with AFPIII. 2020. [DOI:10.1016/j.repbio.2020.03.006]
7. Darvishnia H, SHAMS LM, AKHOUNDI MM, Mobaraki M, Sadeghi M. The Effects of cryopreservation by vitrification on motility, viability and spontaneous acrosome reaction of human spermatozoa. 2008.
8. Amidi F, Pazhohan A, Nashtaei MS, Khodarahmian M, Nekoonam SJC, banking t. The role of antioxidants in sperm freezing: a review. 2016;17(4):745-56. [DOI:10.1007/s10561-016-9566-5]
9. Di Santo M, Tarozzi N, Nadalini M, Borini AJAiu. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. 2011;2012. [DOI:10.1155/2012/854837]
10. Zafarullah M, Li W, Sylvester J, Ahmad MJC, CMLS MLS. Molecular mechanisms of N-acetylcysteine actions. 2003;60(1):6-20. [DOI:10.1007/s000180300001]
11. Minarini A, Ferrari S, Galletti M, Giambalvo N, Perrone D, Rioli G, et al. N-acetylcysteine in the treatment of psychiatric disorders: current status and future prospects. 2017;13(3):279-92. [DOI:10.1080/17425255.2017.1251580]
12. Zhitkovich A. N-acetylcysteine: antioxidant, aldehyde scavenger, and more. ACS Publications; 2019. [DOI:10.1021/acs.chemrestox.9b00152]
13. Sarıözkan S, Bucak MN, Tuncer PB, Ulutaş PA, Bilgen AJC. The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. 2009;58(2):134-8. [DOI:10.1016/j.cryobiol.2008.11.006]
14. Ansari MS, Rakha BA, Malik MF, Andrabi SMH, Ullah N, Iqbal R, et al. Effect of cysteine addition to the freezing extender on the progressive motility, viability, plasma membrane and DNA integrity of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa. 2016;44(1):36-41. [DOI:10.1080/09712119.2014.987292]
15. Organization WH. World health statistics 2010: World Health Organization; 2010.
16. Brito LF, Greene LM, Kelleman A, Knobbe M, Turner RJT. Effect of method and clinician on stallion sperm morphology evaluation. 2011;76(4):745-50. [DOI:10.1016/j.theriogenology.2011.04.007]
17. Nasr-Esfahani MH, Aboutorabi R, Esfandiari E, Mardani MJJoar, genetics. Sperm MTT viability assay: a new method for evaluation of human sperm viability. 2002;19(10):477-82. [DOI:10.1023/A:1020310503143]
18. Singh A, Agarwal A. The role of sperm chromatin integrity and DNA damage on male infertility. The Open Reproductive Science Journal. 2011;3(1). [DOI:10.2174/1874255601103010065]
19. Dariush G, Gholamhossein R, Rouhollah F, Mahmood GS, Abdolhossein S, Mohsen S, et al. The application of ultrasonic vibration in human sperm cryopreservation as a novel method for the modification of physicochemical characteristics of freezing media. 2019;9(1):1-15. [DOI:10.1038/s41598-019-46424-0]
20. TAVALAEE M, TORKI BB, AZADI L, NASR EM. Human sperm cryopreservation update in treatment of infertility: a review study. 2018.
21. Michael A, Alexopoulos C, Pontiki E, Hadjipavlou‐Litina D, Saratsis P, Ververidis H, et al. Effect of N‐acetyl‐L‐cysteine supplementation in semen extenders on semen quality and reactive oxygen species of chilled canine spermatozoa. 2010;45(2):201-7. [DOI:10.1111/j.1439-0531.2008.01202.x]
22. Oeda T, Henkel R, Ohmori H, Schill WBJA. Scavenging effect of N‐acetyl‐L‐cysteine against reactive oxygen species in human semen: a possible therapeutic modality for male factor infertility? 1997;29(3):125-31. [DOI:10.1111/j.1439-0272.1997.tb00305.x]
23. Majzoub A, Agarwal A. Antioxidants in Sperm Cryopreservation. Male Infertility: Springer; 2020. p. 671-8. [DOI:10.1007/978-3-030-32300-4_54]
24. Jannatifar R, Parivar K, Roodbari NH, Nasr-Esfahani MHJRB, Endocrinology. Effects of N-acetyl-cysteine supplementation on sperm quality, chromatin integrity and level of oxidative stress in infertile men. 2019;17(1):1-9. [DOI:10.1186/s12958-019-0468-9]
25. Jannatifar R, Parivar K, Roodbari NH, Nasr-Esfahani MHJIJFS. The effect of N-acetyl-cysteine on the expression of Nrf2 Antioxidant gene in asthenoteratozoospermia men: A clinical trial study. 2020;14(3).
26. Asadpour R, Azari M, Hejazi M, Tayefi H, Zaboli N, editors. Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead. Veterinary research forum: an international quarterly journal; 2013: Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
27. Khalili B, Jafaroghli M, Farshad A, Paresh-Khiavi MJA-AJoAS. The effects of different concentrations of glycine and cysteine on the freezability of Moghani ram spermatozoa. 2010;23(3):318-25. [DOI:10.5713/ajas.2010.90387]
28. Lopes S, Jurisicova A, Sun J-G, Casper RFJHR. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. 1998;13(4):896-900. [DOI:10.1093/humrep/13.4.896]
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Jannatifar R, Piroozmanesh H, Jannatifar Z. Effect of N-acetylcysteine on Human Sperm Parameters and DNA Damage in Frozen-thawed Sperm Samples of Asthenozoospermic Men. Qom Univ Med Sci J 2021; 14 (12) :32-40
URL: http://journal.muq.ac.ir/article-1-2991-en.html

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Volume 14, Issue 12 (February 2021) Back to browse issues page
مجله دانشگاه علوم پزشکی قم Qom University of Medical Sciences Journal
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