Volume 15, Issue 1 (April 2021)
Qom Univ Med Sci J 2021, 15(1): 38-47 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Soleimani Mehranjani M, Delbari N, Ahmadi S. The Effects of Quercetin on the Tissue Quality and Function of Mouse Autotransplanted Ovary. Qom Univ Med Sci J 2021; 15 (1) :38-47
URL: http://journal.muq.ac.ir/article-1-3012-en.html
URL: http://journal.muq.ac.ir/article-1-3012-en.html
1- Department of Biology, Faculty of Science, Arak University, Arak, Iran. , m-soleimani@araku.ac.ir
2- Department of Biology, Faculty of Science, Arak University, Arak, Iran.
2- Department of Biology, Faculty of Science, Arak University, Arak, Iran.
Abstract: (1570 Views)
Background and Objectives: Ischemia-Reperfusion injury, followed by the production of oxygen free radicals and oxidative stress are the main limitations of ovarian tissue transplantation. We aimed to investigate the effects of quercetin as an antioxidant on the quality of autotransplanted mouse ovaries.
Methods In total, 36 female NMRI mice were divided into 3 groups of control, autografted, and autografted + quercetin (50 mg/kg/day). Treatment was performed one day before till 7 days after transplantation and 28 days after transplantation; ovaries were removed for stereological assessments. Serum samples were collected on days 7 and 28 post-transplantation to measure the level of malondialdehyde, total antioxidant capacity, estradiol, and progesterone. The obtained data were analyzed using one-way Analysis of Variance (ANOVA) and Tukey’s test at the significance level of P<0.05.
Results The mean total volume of the ovary, the volume of the cortex, and the volume of the medulla, the mean number of different types of follicles (P<0.001), the level of estradiol (P<0.05), progesterone (P<0.001), and total antioxidant capacity (P<0.01), and the recovery rate of the estrous cycle (P<0.001) significantly decreased in the autografted group, compared to the controls; however, the mentioned parameters significantly increased in the autografted + quercetin group, compared to the autografted group (P<0.01). A significant increase was observed in the level of malondialdehyde on days 7 (P<0.001) and 28 (P<0.01) post-transplantation in the autografted group, compared to the control group; however, this parameter was compensated to the control level in the autografted + quercetin group.
Conclusion The present study findings revealed indicated that quercetin could improve tissue quality and the function of autotransplanted mouse ovaries.
Methods In total, 36 female NMRI mice were divided into 3 groups of control, autografted, and autografted + quercetin (50 mg/kg/day). Treatment was performed one day before till 7 days after transplantation and 28 days after transplantation; ovaries were removed for stereological assessments. Serum samples were collected on days 7 and 28 post-transplantation to measure the level of malondialdehyde, total antioxidant capacity, estradiol, and progesterone. The obtained data were analyzed using one-way Analysis of Variance (ANOVA) and Tukey’s test at the significance level of P<0.05.
Results The mean total volume of the ovary, the volume of the cortex, and the volume of the medulla, the mean number of different types of follicles (P<0.001), the level of estradiol (P<0.05), progesterone (P<0.001), and total antioxidant capacity (P<0.01), and the recovery rate of the estrous cycle (P<0.001) significantly decreased in the autografted group, compared to the controls; however, the mentioned parameters significantly increased in the autografted + quercetin group, compared to the autografted group (P<0.01). A significant increase was observed in the level of malondialdehyde on days 7 (P<0.001) and 28 (P<0.01) post-transplantation in the autografted group, compared to the control group; however, this parameter was compensated to the control level in the autografted + quercetin group.
Conclusion The present study findings revealed indicated that quercetin could improve tissue quality and the function of autotransplanted mouse ovaries.
Type of Study: Original Article |
Subject:
بافت شناسی و جنین شناسی
Received: 2020/12/18 | Accepted: 2021/03/18 | Published: 2021/03/30
Received: 2020/12/18 | Accepted: 2021/03/18 | Published: 2021/03/30
References
1. Dath C, Van Eyck AS, Dolmans MM, Romeu L, Delle Vigne L, Donnez J, et al. Xenotransplantation of human ovarian tissue to nude mice: Comparison between four grafting sites. Hum Reprod. 2010; 25(7):1734-43. Link [DOI:10.1093/humrep/deq131] [PMID] [DOI:10.1093/humrep/deq131]
2. Kawamura K, Cheng Y, Sun YP, Zhai J, Diaz-Garcia C, Simon C, et al. Ovary transplantation: To activate or not to activate. Hum Reprod. 2015; 30(11):2457-60. [DOI:10.1093/humrep/dev211] [PMID] [DOI:10.1093/humrep/dev211]
3. Diaz-Garcia C, Domingo J, Garcia-Velasco JA, Herraiz S, Mirabet V, Iniesta I, et al. Oocyte vitrification versus ovarian cortex transplantation in fertility preservation for adult women undergoing gonadotoxic treatments: A prospective cohort study. Fertil Steril. 2018; 109(3):478-85. [DOI:10.1016/j.fertnstert.2017.11.018] [PMID] [DOI:10.1016/j.fertnstert.2017.11.018]
4. Shiroma ME, Botelho NM, Damous LL, Baracat EC, Soares JM Jr. Melatonin influence in ovary transplantation: Systematic review. J Ovarian Res. 2016; 9(1):33. [DOI:10.1186/s13048-016-0245-8] [PMID] [PMCID] [DOI:10.1186/s13048-016-0245-8]
5. Juranek I, Bezek S. Controversy of free radical hypothesis: Reactive oxygen species-cause or consequence of tissue injury? Gen Physiol Biophys 2005; 24(3):263-78. [PMID]
6. Slegtenhorst BR, Dor FJ, Rodriguez H, Voskuil FJ, Tullius SG. Ischemia/reperfusion injury and its consequences on immunity and inflammation. Curr Transplant Rep. 2014; 1(3):147-54. [DOI:10.1007/s40472-014-0017-6] [PMID] [PMCID] [DOI:10.1007/s40472-014-0017-6]
7. Mahajan N. Fertility preservation in female cancer patients: An overview. J Hum Reprod Sci. 2015; 8(1):3-13. [DOI:10.4103/0974-1208.153119] [PMID] [PMCID] [DOI:10.4103/0974-1208.153119]
8. Aubard Y, Piver P, Cognie Y, Fermeaux V, Poulin N, Driancourt MA. Orthotopic and heterotopic autografts of frozen-thawed ovarian cortex in sheep. Hum Reprod. 1999; 14(8):2149-54. [DOI:10.1093/humrep/14.8.2149] [PMID] [DOI:10.1093/humrep/14.8.2149]
9. Ozkan OV, Yuzbasioglu MF, Ciralik H, Kurutas EB, Yonden Z, Aydin M, et al. Resveratrol, a natural antioxidant, attenuates intestinal ischemia/reperfusion injury in rats. Tohoku J Exp Med. 2009; 218(3):251-8. [DOI:10.1620/tjem.218.251] [PMID] [DOI:10.1620/tjem.218.251]
10. Nugent D, Newton H, Gallivan L, Gosden RG. Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts. J Reprod Fertil. 1998; 114(2):341-6. [DOI:10.1530/jrf.0.1140341] [PMID] [DOI:10.1530/jrf.0.1140341]
11. Atala E, Fuentes J, Wehrhahn MJ, Speisky H. Quercetin and related flavonoids conserve their antioxidant properties despite undergoing chemical or enzymatic oxidation. Food Chem. 2017; 234:479-85. [DOI:10.1016/j.foodchem.2017.05.023] [PMID] [DOI:10.1016/j.foodchem.2017.05.023]
12. Zizkova P, Stefek M, Rackova L, Prnova M, Horakova L. Novel quercetin derivatives: From redox properties to promising treatment of oxidative stress related diseases. Chem Biol Interact. 2017; 265:36-46. [DOI:10.1016/j.cbi.2017.01.019] [PMID] [DOI:10.1016/j.cbi.2017.01.019]
13. Anand David AV, Arulmoli R, Parasuraman S. Overviews of biological importance of quercetin: A bioactive flavonoid. Pharmacogn Rev. 2016; 10(20):84-9. [DOI:10.4103/0973-7847.194044] [PMID] [PMCID] [DOI:10.4103/0973-7847.194044]
14. Xu D, Hu MJ, Wang YQ, Cui YL. Antioxidant activities of quercetin and its complexes for medicinal application. Molecules. 2019; 24(6):1123. [DOI:10.3390/molecules24061123] [PMID] [PMCID] [DOI:10.3390/molecules24061123]
15. Gencer M, Karaca T, Güngör AN, Hacıvelioğlu SÖ, Demirtaş S, Turkon H, et al. The protective effect of quercetin on IMA levels and apoptosis in experimental ovarian ischemia-reperfusion injury. Eur J Obstet Gynecol Reprod Biol. 2014; 177:135-40. [DOI:10.1016/j.ejogrb.2014.03.036] [PMID] [DOI:10.1016/j.ejogrb.2014.03.036]
16. Wang J, Qian X, Gao Q, Lv C, Xu J, Jin H, et al. Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro. J Ovarian Res. 2018; 11(1):51. [DOI:10.1186/s13048-018-0421-0] [PMID] [PMCID] [DOI:10.1186/s13048-018-0421-0]
17. Zohrabi D, Parivar K, Sanati MH, Hayati Roodbari N. [The effect of Quercetin on ovarian tissue of Female Wistar Rats treated with cyclophosphamide and growth indexes of their offspring (Persian)]. J Cell Tissue. 2017; 8(3):285-93. http://jct.araku.ac.ir/article_30363.html?lang=en
18. Shojafar E, Soleimani Mehranjani M, Shariatzadeh SMA. Adipose derived mesenchymal stem cells improve the structure and function of autografted mice ovaries through reducing oxidative stress and inflammation: A stereological and biochemical analysis. Tissue Cell. 2019; 56:23-30. [DOI:10.1016/j.tice.2018.11.005] [PMID] [DOI:10.1016/j.tice.2018.11.005]
19. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52:302-10. [DOI:10.1016/S0076-6879(78)52032-6] [DOI:10.1016/S0076-6879(78)52032-6]
20. Benzie IF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem. 1996; 239(1):70-6. [DOI:10.1006/abio.1996.0292] [PMID] [DOI:10.1006/abio.1996.0292]
21. Mahmoodi M, Soleimani Mehranjani M, Shariatzadeh SM, Eimani H, Shahverdi A. N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress. Reprod Biomed Online. 2015; 30(1):101-10. [DOI:10.1016/j.rbmo.2014.09.013] [PMID] [DOI:10.1016/j.rbmo.2014.09.013]
22. Shojafar E, Soleimani Mehranjani M, Shariatzadeh SMA. Adipose-derived mesenchymal stromal cell transplantation at the graft site improves the structure and function of autografted mice ovaries: A stereological and biochemical analysis. Cytotherapy. 2018; 20(11):1324-36. [DOI:10.1016/j.jcyt.2018.09.006] [PMID] [DOI:10.1016/j.jcyt.2018.09.006]
23. Amable PR, Teixeira MV, Carias RB, Granjeiro JM, Borojevic R. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media. PloS One. 2014; 9(8):e104662. [DOI:10.1371/journal.pone.0104662] [PMID] [PMCID] [DOI:10.1371/journal.pone.0104662]
24. Wong CH, Crack PJ. Modulation of neuro-inflammation and vascular response by oxidative stress following cerebral ischemia-reperfusion injury. Curr Med Chem. 2008; 15(1):1-4. [DOI:10.2174/092986708783330665] [PMID] [DOI:10.2174/092986708783330665]
25. Yilmaz G, Granger DN. Cell adhesion molecules and ischemic stroke. Neurol Res. 2008; 30(8):783-93. [DOI:10.1179/174313208X341085] [PMID] [PMCID] [DOI:10.1179/174313208X341085]
26. Gnoni GV, Paglialonga G, Siculella L. Quercetin inhibits fatty acid and triacylglycerol synthesis in rat‐liver cells. Eur J Clin Invest. 2009; 39(9):761-8. [DOI:10.1111/j.1365-2362.2009.02167.x] [PMID] [DOI:10.1111/j.1365-2362.2009.02167.x]
27. Zhou DS, Liang ZQ, Qin Q, Zhang MH, Li SL. [Therapeutic efficacy and mechanisms of quercetin in a rat model of nonalcoholic fatty liver disease (Chinese)]. Zhonghua Gan Zang Bing Za Zhi. 2013; 21(2):134-7. [PMID] [DOI:10.1111/j.1365-2362.2009.02167.x] [DOI:10.1111/j.1365-2362.2009.02167.x]
28. Li F, Tao Y, Zhang Y, Li Y, Fang F, Liu Y, et al. Follicle growth and oocyte development after ovary transplantation into back muscle of immune-intact adult castrated male mice. Reproduction. 2010; 140(3):465-76. [DOI:10.1530/REP-10-0076] [PMID] [DOI:10.1530/REP-10-0076]
29. Greenfeld CR, Babus JK, Furth PA, Marion S, Hoyer PB, Flaws JA. BAX is involved in regulating follicular growth, but is dispensable for follicle atresia in adult mouse ovaries. Reproduction. 2007; 133(1):107-16. [DOI:10.1530/REP-06-0144] [PMID] [DOI:10.1530/REP-06-0144]
30. Pourteymour Fard Tabrizi F, Hajizadeh-Sharafabad F, Vaezi M, Jafari-Vayghan H, Alizadeh M, Maleki V. Quercetin and polycystic ovary syndrome, current evidence and future directions: A systematic review. J Ovarian Res. 2020; 13(1):11. [DOI:10.1186/s13048-020-0616-z] [PMID] [PMCID] [DOI:10.1186/s13048-020-0616-z]
31. Slegtenhorst BR, Dor FJ, Rodriguez H, Voskuil FJ, Tullius SG. Ischemia/reperfusion injury and its consequences on immunity and inflammation. Curr Transplant Rep. 2014; 1(3):147-54. [DOI:10.1007/s40472-014-0017-6] [PMID] [PMCID] [DOI:10.1007/s40472-014-0017-6]
32. Rumley AG, Paterson JR. Analytical aspects of antioxidants and free radical activity in clinical biochemistry. Ann Clin Biochem. 1998; 35(Pt 2):181-200. [DOI:10.1177/000456329803500202] [PMID] [DOI:10.1177/000456329803500202]
33. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med. 1991; 11(1):81-128. [DOI:10.1016/0891-5849(91)90192-6] [DOI:10.1016/0891-5849(91)90192-6]
34. Bryszewska M, Zavodnik IB, Niekurzak A, Szosland K. Oxidative processes in red blood cells from normal and diabetic individuals. Biochem Mol Biol Int. 1995; 37(2):345-54. [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Owned by Qom University of Medical Sciences
Published by Qom University of Medical Sciences Press
eISSN: 2008-1375
pISSN: 1735-7799
Email: journal[at]muq.ac.ir
j.mua.ac[at]gmail.com
